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Literature summary for 1.1.1.27 extracted from

  • Fields, P.A.; Strothers, C.M.; Mitchell, M.A.
    Function of muscle-type lactate dehydrogenase and citrate synthase of the Galapagos marine iguana, Amblyrhynchus cristatus, in relation to temperature (2008), Comp. Biochem. Physiol. B, 150, 62-73.
    View publication on PubMed
Search for more literature for 1.1.1.27

Cloned(Commentary)

Cloned (Comment) Organism
gene ldh-a, DNA and amino acid sequence determination and analysis Amblyrhynchus cristatus
gene ldh-a, DNA and amino acid sequence determination and analysis Iguana iguana

Protein Variants

Protein Variants Comment Organism
I283V | site-directed mutagenesis, the mutation switches the nature of the residue from Amblyrhynchus cristatus to that of Iguana iguana, another Galapagos marine iguana, the mutation does not affect enzyme kinetics, overview Amblyrhynchus cristatus
T9A site-directed mutagenesis, the mutation switches the nature of the residue from Amblyrhynchus cristatus to that of Iguana iguana, another Galapagos marine iguana, the mutation does not affect enzyme kinetics, overview Amblyrhynchus cristatus
T9A/I283V site-directed mutagenesis, the mutations switch the nature of the residues from Amblyrhynchus cristatus to those of Iguana iguana, another Galapagos marine iguana, the mutation affects enzyme kinetics decreasing Km for pyruvate and kcat, overview Amblyrhynchus cristatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-lactate + NAD+ Amblyrhynchus cristatus
-
 pyruvate + NADH + H+
-
 r
(S)-lactate + NAD+ Iguana iguana
-
 pyruvate + NADH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Amblyrhynchus cristatus Q0GND9 Galapagos marine iguana, gene ldh-a
-
Iguana iguana Q0GND8 common iguana
-

Source Tissue

Source Tissue Comment Organism Textmining
skeletal muscle muscle isozyme Amblyrhynchus cristatus
-
skeletal muscle muscle isozyme Iguana iguana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-lactate + NAD+
-
 Amblyrhynchus cristatus pyruvate + NADH + H+
-
 r
(S)-lactate + NAD+
-
 Iguana iguana pyruvate + NADH + H+
-
 r
additional information amino acid sequence, structure and kinetic comparison of L-LDH with citrate synthase, EC 2.3.3.1, overview Amblyrhynchus cristatus ?
-
 ?
additional information amino acid sequence, structure and kinetic comparison of L-LDH with citrate synthase, EC 2.3.3.1, overview Iguana iguana ?
-
 ?

Subunits

Subunits Comment Organism
More molecular modeling of the three-dimensional enzyme structure in comparison to the enzyme structure from Amblyrhynchus cristatus, overview Iguana iguana
More molecular modeling of the three-dimensional enzyme structure in comparison to the enzyme structure from Iguana iguana, overview Amblyrhynchus cristatus

Synonyms

Synonyms Comment Organism
A4-LDH
-
 Amblyrhynchus cristatus
A4-LDH
-
 Iguana iguana
anaerobic lactate dehydrogenase
-
 Amblyrhynchus cristatus
anaerobic lactate dehydrogenase
-
 Iguana iguana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Amblyrhynchus cristatus
30
-
 assay at Iguana iguana

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
14 46 in vivo temperature Amblyrhynchus cristatus
14 46 in vivo temperature Iguana iguana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Amblyrhynchus cristatus
7
-
 assay at Iguana iguana

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Amblyrhynchus cristatus
NAD+
-
 Iguana iguana
NADH
-
 Amblyrhynchus cristatus
NADH
-
 Iguana iguana