Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-fructose 1,6-bisphosphate | allosteric | Thermoanaerobacter ethanolicus | |
Triton X-100 | 23% activation at 0.1% | Thermoanaerobacter ethanolicus | |
Urea | 9% activation at 0.1% | Thermoanaerobacter ethanolicus |
Cloned (Comment) | Organism |
---|---|
gene ldhL, DNA and amino acid sequence determination and analysis, overexpression of the His-tagged enzyme in Escherichia coli | Thermoanaerobacter ethanolicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 10% inhibition at 1 mM | Thermoanaerobacter ethanolicus | |
Ag+ | complete inhibition at 1 mM | Thermoanaerobacter ethanolicus | |
Cu2+ | complete inhibition at 1 mM | Thermoanaerobacter ethanolicus | |
DTT | 19% inhibition at 1 mM | Thermoanaerobacter ethanolicus | |
Fe2+ | 74% inhibition at 1 mM | Thermoanaerobacter ethanolicus | |
Fe3+ | complete inhibition at 1 mM | Thermoanaerobacter ethanolicus | |
NAD+ | 7% inhibition at 0.5 mM | Thermoanaerobacter ethanolicus | |
SDS | complete inhibition at 0.1% | Thermoanaerobacter ethanolicus | |
Zn2+ | complete inhibition at 1 mM | Thermoanaerobacter ethanolicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, the enzyme is allosteric in presence of D-fructose 1,6-bisphosphate, overview | Thermoanaerobacter ethanolicus | |
0.18 | - |
pyruvate | pH 5.8, 60°C, recombinant enzyme | Thermoanaerobacter ethanolicus | |
60 | - |
L-lactate | pH 7.0, 70°C, recombinant enzyme | Thermoanaerobacter ethanolicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | not or poorly affected by 1 mM of EDTA, ATP, Mg2+, Co2+, Ca2+, Mn2+, and Ni2+ | Thermoanaerobacter ethanolicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lactate + NAD+ | Thermoanaerobacter ethanolicus | - |
pyruvate + NADH + H+ | - |
r | |
L-lactate + NAD+ | Thermoanaerobacter ethanolicus JW 200 | - |
pyruvate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoanaerobacter ethanolicus | B1A4R6 | gene ldhL | - |
Thermoanaerobacter ethanolicus JW 200 | B1A4R6 | gene ldhL | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography to homogeneity | Thermoanaerobacter ethanolicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lactate + NAD+ | - |
Thermoanaerobacter ethanolicus | pyruvate + NADH + H+ | - |
r | |
L-lactate + NAD+ | - |
Thermoanaerobacter ethanolicus JW 200 | pyruvate + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
More | TeLdhL contains a GXGXXG motif common to most NAD-linked dehydrogenases close to the N-terminus, and a putative signal peptide with cleavage site between residue 26 and 27, deletion of the putative signal peptide sequence leads to nonexpression of TeLdhL | Thermoanaerobacter ethanolicus |
Synonyms | Comment | Organism |
---|---|---|
L-(+)-lactate dehydrogenase | - |
Thermoanaerobacter ethanolicus |
L-LDH | - |
Thermoanaerobacter ethanolicus |
TeLdhL | - |
Thermoanaerobacter ethanolicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
pyruvate reduction, recombinant enzyme | Thermoanaerobacter ethanolicus |
70 | - |
lactate oxidation, recombinant enzyme | Thermoanaerobacter ethanolicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
purified recombinant enzyme, 1-5 h, pH 5.8-8.2, completely stable | Thermoanaerobacter ethanolicus |
80 | - |
purified recombinant enzyme, half-life is 1.5 h | Thermoanaerobacter ethanolicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.8 | - |
pyruvate reduction, recombinant enzyme | Thermoanaerobacter ethanolicus |
7 | - |
lactate oxidation, recombinant enzyme | Thermoanaerobacter ethanolicus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
incubation of TeLdhL at lower pH results in a rapid inactivation of the enzyme | Thermoanaerobacter ethanolicus |
5.8 | 8.2 | purified recombinant enzyme, 1-5 h, 70°C, completely stable | Thermoanaerobacter ethanolicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Thermoanaerobacter ethanolicus | |
NADH | - |
Thermoanaerobacter ethanolicus |