Protein Variants | Comment | Organism |
---|---|---|
additional information | overexpression of 17beta-hydroxysteroid dehydrogenase type 7 in ERG27-deleted yeast cells. Recombinant strains grow more slowly than the control yeast ERG27-overexpressing strain on sterol-deficient media, whereas the growth rate is normal on media supplemented with a 3-ketoreductase substrate. Mammalian steroid-3-ketoreductase expressed in yeast shows full enzymatic functioanlity but is unable to support the functionality of yeast oxidosqualene cyclase as ERG27 does | Mus musculus |
additional information | overexpression of 17beta-hydroxysteroid dehydrogenase type 7 in ERG27-deleted yeast cells. Recombinant strains grow more slowly than the control yeast ERG27-overexpressing strain on sterol-deficient media, whereas the growth rate is normal on media supplemented with a 3-ketoreductase substrate. Mammalian steroid-3-ketoreductase expressed in yeast shows full enzymatic functioanlity but is unable to support the functionality of yeast oxidosqualene cyclase as ERG27 does | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P56937 | - |
- |
Mus musculus | O88736 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
NS0 cell | - |
Mus musculus | - |
Synonyms | Comment | Organism |
---|---|---|
17beta-hydroxysteroid dehydrogenase type 7 | - |
Mus musculus |
17beta-hydroxysteroid dehydrogenase type 7 | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | in NS0 cells deficient in 17beta-hydroxysteroid dehydrogenase type 7 activity, oxidosqualene cyclase is fully active. The protective role of the 3-ketoreductase in yeasts with respect to oxidosqualene cyclase activity is not conserved in mammals | Mus musculus |