General Stability | Organism |
---|---|
highly instable enzyme, in crude extracts, half-life of activity is 25 h at 4°C, 0.25% activity remains after 7 d, activity depends completely on the presence of 2-mercaptoethanol | Rauvolfia serpentina |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 1 mM, 71% inhibition | Rauvolfia serpentina | |
EDTA | 1 mM, 77% inhibition | Rauvolfia serpentina | |
NADP+ | noncompetitive inhibition | Rauvolfia serpentina | |
neocuproin | 1 mM, 65% inhibition | Rauvolfia serpentina |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0167 | - |
vellosimine | - |
Rauvolfia serpentina | |
0.02 | - |
NADPH | - |
Rauvolfia serpentina |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
- |
Rauvolfia serpentina |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Catharanthus roseus | - |
- |
- |
no activity in Alstonia scholaris | - |
- |
- |
no activity in Carissa macrocarpa | - |
- |
- |
no activity in Catharanthus roseus | - |
- |
- |
no activity in Coleus blumei | - |
- |
- |
no activity in Malus sylvestris | - |
- |
- |
no activity in Nicotiana sylvestris | - |
- |
- |
no activity in Picralima nitida | - |
- |
- |
no activity in Rhazya stricta | - |
- |
- |
no activity in Stemmadenia tomentosa | - |
- |
- |
no activity in Tabernanthe iboga | - |
- |
- |
no activity in Voacanga africana | - |
- |
- |
Rauvolfia serpentina | - |
- |
- |
Rauvolfia verticillata | - |
- |
- |
Rauvolfia vomitoria | - |
- |
- |
Vinca herbacea | - |
- |
- |
Vinca minor | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rauvolfia serpentina |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | - |
Catharanthus roseus | - |
cell suspension culture | - |
Rauvolfia vomitoria | - |
cell suspension culture | - |
Rauvolfia serpentina | - |
cell suspension culture | - |
Rauvolfia verticillata | - |
cell suspension culture | - |
Vinca minor | - |
cell suspension culture | - |
Vinca herbacea | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00018 | - |
vellosimine reduction in cell suspension culture | Vinca herbacea |
0.0006 | - |
vellosimine reduction in cell suspension culture | Vinca minor |
0.0012 | - |
- |
Rauvolfia serpentina |
0.013 | - |
vellosimine reduction in cell suspension culture | Rauvolfia vomitoria |
0.0156 | - |
vellosimine reduction in cell suspension culture | Catharanthus roseus |
0.0192 | - |
vellosimine reduction in cell suspension culture | Rauvolfia verticillata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
16-epi-vellosimine + NADPH | - |
Rauvolfia serpentina | ? | - |
? | |
deoxyajmalal B + NADPH | - |
Rauvolfia serpentina | ? | - |
? | |
gardneral + NADPH | - |
Rauvolfia serpentina | ? | - |
? | |
geissoschizal + NADPH | - |
Rauvolfia serpentina | ? | - |
? | |
N-methylgardneral + NADPH | - |
Rauvolfia serpentina | ? | - |
? | |
vellosimine + NADPH | - |
Rauvolfia serpentina | 10-deoxysarpagine + NADP+ | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
- |
Rauvolfia serpentina |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8.5 | 50% of maximal activity at pH 6.8 and pH 7.6 respectively | Rauvolfia serpentina |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | absolutely specific for NADPH | Rauvolfia serpentina |