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Literature summary for 1.1.1.274 extracted from

  • Miller, J.V.; Estell, D.A.; Lazarus, R.A.
    Purification and characterization of 2,5-diketo-D-gluconate reductase from Corynebacterium sp (1987), J. Biol. Chem., 262, 9016-9020.
    View publication on PubMed
Search for more literature for 1.1.1.274

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 0.5 mM, strong inhibition Corynebacterium sp.
Fe3+ 0.5 mM, strong inhibition Corynebacterium sp.
NADP+ competitive product inhibition Corynebacterium sp.
Ni2+ 0.5 mM, strong inhibition Corynebacterium sp.
Zn2+ 0.5 mM, strong inhibition Corynebacterium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 NADPH
-
 Corynebacterium sp.
2.2
-
 glycerol
-
 Corynebacterium sp.
26
-
 2,5-didehydro-D-gluconate
-
 Corynebacterium sp.
155
-
 5-keto-D-fructose
-
 Corynebacterium sp.
160
-
 dihydroxyacetone
-
 Corynebacterium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29992
-
 1 * 29992, deduced from nucleotide sequence Corynebacterium sp.
34000
-
 1 * 34000, SDS-PAGE Corynebacterium sp.
35000
-
 gel filtration Corynebacterium sp.

Organism

Organism UniProt Comment Textmining
Corynebacterium sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-cellulose, Cibacron blue F3GA agarose, gel filtration Corynebacterium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.38
-
-
 Corynebacterium sp.

Storage Stability

Storage Stability Organism
-70°C, 20 mM Tris-HCl, pH 7.5, over 6 months, no loss of activity Corynebacterium sp.
4°C, pH 6.5-7.5, at least 2 months, no loss of activity Corynebacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,5-didehydro-D-gluconate + NADH 170fold lower reduction rate with NADH compared to NADPH Corynebacterium sp. 2-keto-L-gulonate + NAD+
-
 ?
2,5-didehydro-D-gluconate + NADH 170fold lower reduction rate with NADH compared to NADPH Corynebacterium sp. ATCC 31090 2-keto-L-gulonate + NAD+
-
 ?
2,5-didehydro-D-gluconate + NADPH stereospecific reduction to 2-keto-L-gulonate, no activity with D-fructose, L-sorbose, 5-keto-D-gluconate, 2-keto-L-gulonate, 2-keto-D-gluconate, pyruvate or hydroxypyruvate Corynebacterium sp. 2-keto-L-gulonate + NADP+
-
 r
2,5-didehydro-D-gluconate + NADPH stereospecific reduction to 2-keto-L-gulonate, no activity with D-fructose, L-sorbose, 5-keto-D-gluconate, 2-keto-L-gulonate, 2-keto-D-gluconate, pyruvate or hydroxypyruvate Corynebacterium sp. ATCC 31090 2-keto-L-gulonate + NADP+
-
 r
5-keto-D-fructose + NADPH
-
 Corynebacterium sp. L-sorbose + NADP+
-
 ?
5-keto-D-fructose + NADPH
-
 Corynebacterium sp. ATCC 31090 L-sorbose + NADP+
-
 ?
dihydroxyacetone + NADPH
-
 Corynebacterium sp. glycerol + NADP+
-
 ?
dihydroxyacetone + NADPH
-
 Corynebacterium sp. ATCC 31090 glycerol + NADP+
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 34000, SDS-PAGE Corynebacterium sp.
monomer 1 * 29992, deduced from nucleotide sequence Corynebacterium sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
8.33
-
 2,5-didehydro-D-gluconate
-
 Corynebacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4
-
 reduction of 2,5-didehydro-D-gluconate Corynebacterium sp.
9.2
-
 oxidation of 2-keto-L-gulonate Corynebacterium sp.

pH Range

pH Minimum pH Maximum Comment Organism
4.5 9.3 reduction of 2,5-didehydro-D-gluconate Corynebacterium sp.
8 10.3 oxidation of 2-keto-L-gulonate Corynebacterium sp.

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Corynebacterium sp.
NADPH
-
 Corynebacterium sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.026
-
 NADP+
-
 Corynebacterium sp.