Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli JM109 | Escherichia coli |
overexpression in Escherichia coli JM109 | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | substrate specificity | Escherichia coli | |
additional information | - |
additional information | substrate specificity | Saccharomyces cerevisiae | |
0.5 | - |
NADP+ | pH 9.0, 30°C | Saccharomyces cerevisiae | |
0.54 | - |
NADP+ | pH 9.0, 30°C | Escherichia coli | |
3 | - |
L-allo-threonine | pH 9.0, 30°C | Saccharomyces cerevisiae | |
29 | - |
L-allo-threonine | pH 9.0, 30°C | Escherichia coli | |
40 | - |
L-serine | pH 9.0, 30°C | Escherichia coli | |
95 | - |
L-serine | pH 9.0, 30°C | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
4 * 27000, recombinant enzyme, SDS-PAGE | Escherichia coli |
29000 | - |
4 * 29000, recombinant enzyme, SDS-PAGE | Saccharomyces cerevisiae |
105000 | - |
recombinant enzyme, gel filtration | Escherichia coli |
110000 | - |
recombinant enzyme, gel filtration | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine + NADP+ | Escherichia coli | - |
2-aminomalonate semialdehyde + NADPH | - |
? | |
L-serine + NADP+ | Saccharomyces cerevisiae | - |
2-aminomalonate semialdehyde + NADPH | - |
? | |
L-serine + NADP+ | Saccharomyces cerevisiae YMR226C | - |
2-aminomalonate semialdehyde + NADPH | - |
? | |
L-serine + NADP+ | Escherichia coli YdfG | - |
2-aminomalonate semialdehyde + NADPH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
YdfG | - |
Escherichia coli YdfG | - |
YdfG | - |
Saccharomyces cerevisiae | - |
YMR226C | - |
Saccharomyces cerevisiae YMR226C | - |
YMR226C | - |
Purification (Comment) | Organism |
---|---|
recombinant from overproducing Escherichia coli, to homogeneity | Escherichia coli |
recombinant from overproducing Escherichia coli, to homogeneity | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.4 | - |
purified recombinant enzyme | Escherichia coli |
6 | - |
purified recombinant enzyme | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-methyl-DL-serine + NADP+ | 59% activity compared to L-serine | Escherichia coli | 2-amino-2-methyl-3-oxopropanoic acid + NADPH | - |
? | |
2-methyl-DL-serine + NADP+ | 21% activity compared to L-serine | Saccharomyces cerevisiae | 2-amino-2-methyl-3-oxopropanoic acid + NADPH | - |
? | |
3-hydroxypropanoate + NADP+ | 23% activity compared to L-serine | Escherichia coli | 3-oxopropanoate + NADPH | - |
? | |
3-hydroxypropanoate + NADP+ | 13% activity compared to L-serine | Saccharomyces cerevisiae | 3-oxopropanoate + NADPH | - |
? | |
D-3-hydroxybutyrate + NADP+ | 1% activity compared to L-serine | Escherichia coli | 3-oxobutyrate + NADPH | - |
? | |
D-3-hydroxybutyrate + NADP+ | 12% activity compared to L-serine | Saccharomyces cerevisiae | 3-oxobutyrate + NADPH | - |
? | |
D-3-hydroxyisobutyrate + NADP+ | 90% activity compared to L-serine | Escherichia coli | 2-methyl-3-oxopropanoic acid + NADPH | - |
? | |
D-3-hydroxyisobutyrate + NADP+ | 78% activity compared to L-serine | Saccharomyces cerevisiae | 2-methyl-3-oxopropanoic acid + NADPH | - |
? | |
D-glycerate + NADP+ | 48% activity compared to L-serine | Escherichia coli | ? | - |
? | |
D-glycerate + NADP+ | 6% activity compared to L-serine | Saccharomyces cerevisiae | ? | - |
? | |
D-serine + NADP+ | 83% activity compared to L-serine | Escherichia coli | 2-aminomalonate semialdehyde + NADPH | - |
? | |
D-serine + NADP+ | 71% activity compared to L-serine | Saccharomyces cerevisiae | 2-aminomalonate semialdehyde + NADPH | - |
? | |
D-serine + NADP+ | 71% activity compared to L-serine | Saccharomyces cerevisiae YMR226C | 2-aminomalonate semialdehyde + NADPH | - |
? | |
D-serine + NADP+ | 83% activity compared to L-serine | Escherichia coli YdfG | 2-aminomalonate semialdehyde + NADPH | - |
? | |
D-threonine + NADP+ | 156% activity compared to L-serine | Escherichia coli | D-2-amino-3-oxobutanoic acid + NADPH | - |
? | |
D-threonine + NADP+ | 478% activity compared to L-serine | Saccharomyces cerevisiae | D-2-amino-3-oxobutanoic acid + NADPH | - |
? | |
L-3-hydroxybutyrate + NADP+ | 85% activity compared to L-serine | Saccharomyces cerevisiae | 3-oxobutyrate + NADPH | - |
? | |
L-3-hydroxybutyrate + NADP+ | 9% activity compared to L-serine | Escherichia coli | 3-oxobutyrate + NADPH | - |
? | |
L-3-hydroxyisobutyrate + NADP+ | 96% activity compared to L-serine | Escherichia coli | 2-methyl-3-oxopropanoic acid + NADPH | - |
? | |
L-3-hydroxyisobutyrate + NADP+ | 182% activity compared to L-serine | Saccharomyces cerevisiae | 2-methyl-3-oxopropanoic acid + NADPH | - |
? | |
L-allo-threonine + NADP+ | best substrate | Escherichia coli | L-2-amino-3-ketobutyrate + NADPH | L-2-amino-3-ketobutyrate spontaneously decarboxylates into aminoacetone | ? | |
L-allo-threonine + NADP+ | best substrate | Saccharomyces cerevisiae | L-2-amino-3-ketobutyrate + NADPH | L-2-amino-3-ketobutyrate spontaneously decarboxylates into aminoacetone | ? | |
L-allo-threonine + NADP+ | 137% activity compared to L-serine | Escherichia coli | L-2-amino-3-ketobutyrate + NADPH | L-2-amino-3-ketobutyrate spontaneously decarboxylates into aminoacetone | ? | |
L-allo-threonine + NADP+ | 397% activity compared to L-serine | Saccharomyces cerevisiae | L-2-amino-3-ketobutyrate + NADPH | L-2-amino-3-ketobutyrate spontaneously decarboxylates into aminoacetone | ? | |
L-glycerate + NADP+ | 28% activity compared to L-serine | Escherichia coli | ? | - |
? | |
L-glycerate + NADP+ | 5% activity compared to L-serine | Saccharomyces cerevisiae | ? | - |
? | |
L-glycerate + NADP+ | 5% activity compared to L-serine | Saccharomyces cerevisiae YMR226C | ? | - |
? | |
L-glycerate + NADP+ | 28% activity compared to L-serine | Escherichia coli YdfG | ? | - |
? | |
L-serine + NADP+ | - |
Escherichia coli | 2-aminomalonate semialdehyde + NADPH | - |
? | |
L-serine + NADP+ | - |
Saccharomyces cerevisiae | 2-aminomalonate semialdehyde + NADPH | - |
? | |
L-serine + NADP+ | - |
Saccharomyces cerevisiae YMR226C | 2-aminomalonate semialdehyde + NADPH | - |
? | |
L-serine + NADP+ | - |
Escherichia coli YdfG | 2-aminomalonate semialdehyde + NADPH | - |
? | |
additional information | no activity with DL-homoserine, O-methyl-L-serine, N-acetyl-DL-serine, DL-isoserine, DL-threo-3-phenylserine, malonate, DL-malate, DL-lactate, DL-tartrate, D-gluconate, citrate, isocitrate, glycolate, glycerol | Escherichia coli | ? | - |
? | |
additional information | no activity with DL-homoserine, O-methyl-L-serine, N-acetyl-DL-serine, DL-isoserine, DL-threo-3-phenylserine, malonate, DL-malate, DL-lactate, DL-tartrate, D-gluconate, citrate, isocitrate, glycolate, glycerol | Saccharomyces cerevisiae | ? | - |
? | |
additional information | L-threonine, D-allo-threonine are poor substrates | Escherichia coli | ? | - |
? | |
additional information | L-threonine, D-allo-threonine are poor substrates | Saccharomyces cerevisiae | ? | - |
? | |
additional information | no activity with DL-homoserine, O-methyl-L-serine, N-acetyl-DL-serine, DL-isoserine, DL-threo-3-phenylserine, malonate, DL-malate, DL-lactate, DL-tartrate, D-gluconate, citrate, isocitrate, glycolate, glycerol | Saccharomyces cerevisiae YMR226C | ? | - |
? | |
additional information | L-threonine, D-allo-threonine are poor substrates | Saccharomyces cerevisiae YMR226C | ? | - |
? | |
additional information | no activity with DL-homoserine, O-methyl-L-serine, N-acetyl-DL-serine, DL-isoserine, DL-threo-3-phenylserine, malonate, DL-malate, DL-lactate, DL-tartrate, D-gluconate, citrate, isocitrate, glycolate, glycerol | Escherichia coli YdfG | ? | - |
? | |
additional information | L-threonine, D-allo-threonine are poor substrates | Escherichia coli YdfG | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 27000, recombinant enzyme, SDS-PAGE | Escherichia coli |
tetramer | 4 * 29000, recombinant enzyme, SDS-PAGE | Saccharomyces cerevisiae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
10 min, 0.1 M potassium phosphate, pH 7.4, 0.01% 2-mercaptoethanol, 10% glycerol, stable | Saccharomyces cerevisiae |
55 | - |
10 min, 0.1 M potassium phosphate, pH 7.4, 0.01% 2-mercaptoethanol, 10% glycerol, stable | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | substrate specificity | Escherichia coli | |
additional information | - |
additional information | substrate specificity | Saccharomyces cerevisiae | |
4.2 | - |
L-serine | pH 9.0, 30°C | Escherichia coli | |
5.76 | - |
L-allo-threonine | pH 9.0, 30°C | Escherichia coli | |
11 | - |
L-serine | pH 9.0, 30°C | Saccharomyces cerevisiae | |
43.8 | - |
L-allo-threonine | pH 9.0, 30°C | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
oxidation of L-serine | Escherichia coli |
8.5 | - |
oxidation of L-serine | Saccharomyces cerevisiae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 10 | 10 min, 40°C, stable | Escherichia coli |
7.5 | 10.5 | 10 min, 40°C, stable | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | dependent on | Escherichia coli | |
NADP+ | dependent on | Saccharomyces cerevisiae | |
NADP+ | NAD+ cannot replace NADP+ | Escherichia coli | |
NADP+ | NAD+ cannot replace NADP+ | Saccharomyces cerevisiae |