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Literature summary for 1.1.1.279 extracted from
- Purification and characterization of two oxidoreductases involved in the enantioselective reduction of 3-oxo, 4-oxo and 5-oxo esters in bakers yeast (1988), Eur. J. Biochem., 172, 633-639.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 8-hydroxyquinoline 5-sulfonic acid | - |
Saccharomyces cerevisiae |  |
| HgCl2 | - |
Saccharomyces cerevisiae | |
| o-phenanthroline | - |
Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2 | - |
ethyl 3-oxohexanoate | - |
Saccharomyces cerevisiae | |
| 17 | - |
ethyl 3-oxobutanoate | - |
Saccharomyces cerevisiae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Saccharomyces cerevisiae | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 200000 | - |
x * 210000 + x * 200000, SDS-PAGE | Saccharomyces cerevisiae |
| 210000 | - |
x * 210000 + x * 200000, SDS-PAGE | Saccharomyces cerevisiae |
| 780000 | 800000 | gel filtration | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using streptomycin sulfate treatment, Sephadex G-25 filtration, DEAE-Sepharose CL-6B chromatography, Sephadex G-150 filtration, Sepharose 6B filtration and hydroxyapatite chromatography | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.958 | - |
- |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ethyl 3-oxobutanoate + NADPH | - |
Saccharomyces cerevisiae | ethyl (R)-3-hydroxybutanoate + NADP+ | - |
? | |
| ethyl 3-oxohexanoate + NADPH | - |
Saccharomyces cerevisiae | ethyl (R)-3-hydroxyhexanoate + NADP+ | - |
? | |
| additional information | specific for 3-oxo-esters, no reaction with 3-oxo-acids | Saccharomyces cerevisiae | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| oligomer | x * 210000 + x * 200000, SDS-PAGE | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.1 | - |
- |
Saccharomyces cerevisiae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
rapid decrease of activity above | Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | specific for | Saccharomyces cerevisiae | |
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