Application | Comment | Organism |
---|---|---|
synthesis | generating GDP-rhamnose for in vitro rhamnosylation of glycoproteins and glycopeptides | Pseudomonas aeruginosa |
synthesis | the functional expression of the Pseudomonas aeruginosa enzyme in Saccharomyces cerevisiae will provide a tool for generating GDP-rhamnose for in vitro rhamnosylation of glycoprotein and glycopeptides | Pseudomonas aeruginosa |
synthesis | generation of GDP-rhamnose by coexpression of enzyme with GDP-mannose-4,6-dehydratase in Saccharomyces cerevisiae | Pseudomonas aeruginosa |
Cloned (Comment) | Organism |
---|---|
expression in Saccharomyces cerevisiae | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33900 | - |
x * 34000, SDS-PAGE of FLAG-tagged recombinant enzyme, x * 33900, calculated | Pseudomonas aeruginosa |
34000 | - |
x * 34000, recombinant FLAG-tagged enzyme | Pseudomonas aeruginosa |
34000 | - |
x * 34000, SDS-PAGE of FLAG-tagged recombinant enzyme, x * 33900, calculated | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-4-dehydro-6-deoxy-D-mannose + NADPH | Pseudomonas aeruginosa | - |
GDP-6-deoxy-D-mannose + NADP+ | - |
? | |
GDP-6-deoxy-D-mannose + NAD(P)+ | Pseudomonas aeruginosa | - |
GDP-4-dehydro-6-deoxy-D-mannose + NAD(P)H | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Pseudomonas aeruginosa | - |
expression in Saccharomyces cerevisiae | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-4-dehydro-6-deoxy-D-mannose + NADPH | - |
Pseudomonas aeruginosa | GDP-6-deoxy-D-mannose + NADP+ | - |
? | |
GDP-6-deoxy-D-lyxo-4-hexulose + NADH + H+ | - |
Pseudomonas aeruginosa | GDP-alpha-D-rhamnose + NAD+ | - |
ir | |
GDP-6-deoxy-D-lyxo-4-hexulose + NADPH + H+ | - |
Pseudomonas aeruginosa | GDP-alpha-D-rhamnose + NADP+ | - |
ir | |
GDP-6-deoxy-D-mannose + NAD(P)+ | - |
Pseudomonas aeruginosa | GDP-4-dehydro-6-deoxy-D-mannose + NAD(P)H | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 34000, recombinant FLAG-tagged enzyme | Pseudomonas aeruginosa |
? | x * 34000, SDS-PAGE of FLAG-tagged recombinant enzyme, x * 33900, calculated | Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas aeruginosa | |
NADH | - |
Pseudomonas aeruginosa | |
NADP+ | - |
Pseudomonas aeruginosa | |
NADPH | - |
Pseudomonas aeruginosa |