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Literature summary for 1.1.1.283 extracted from

  • Inoue, Y.; Rhee, H.; Watanabe, K.; Murata, K.; Kimura, A.
    Metabolism of 2-oxoaldehyde in mold. Purification and characterization of two methylglyoxal reductases from Aspergillus niger (1988), Eur. J. Biochem., 171, 213-218.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
iodoacetate activation of enzyme MGR I, inhibition of enzyme MGR II Aspergillus niger
N-ethylmaleimide activation of enzyme MGR I, inhibition of enzyme MGR II Aspergillus niger
p-chloromercuribenzoate activation of enzyme MGR I, inhibition of enzyme MGR II Aspergillus niger

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol
-
Aspergillus niger
Ca2+ enzymes MGR I (slightly), MGR II Aspergillus niger
Co2+ activates enzyme MGR I, slightly inhibits enzyme MGR II Aspergillus niger
Cu2+ enzymes MGR I and MGR II Aspergillus niger
dithiothreitol
-
Aspergillus niger
EDTA
-
Aspergillus niger
Glyoxal in absence of NADPH Aspergillus niger
iodoacetate activation of enzyme MGR I, inhibition of enzyme MGR II Aspergillus niger
methylglyoxal in absence of NADPH Aspergillus niger
Mg2+ enzymes MGR I and MGR II Aspergillus niger
Mn2+ enzymes MGR I, MGR II Aspergillus niger
N-ethylmaleimide activation of enzyme MGR I, inhibition of enzyme MGR II Aspergillus niger
NADP+
-
Aspergillus niger
Ni2+ enzyme MGR I Aspergillus niger
p-chloromercuribenzoate activation of enzyme MGR I, inhibition of enzyme MGR II Aspergillus niger
Phenylglyoxal in absence of NADPH Aspergillus niger
Zn2+ enzyme MGR I Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0068
-
NADPH enzyme MGR II Aspergillus niger
0.054
-
NADPH enzyme MGR I Aspergillus niger
0.7
-
methylglyoxal
-
Aspergillus niger
1.43
-
methylglyoxal enzyme MGR I Aspergillus niger
4.35
-
Phenylglyoxal enzyme MGR II Aspergillus niger
10
-
Glyoxal enzyme MGR II Aspergillus niger
15.4
-
methylglyoxal enzyme MGR I Aspergillus niger

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates enzyme MGR I, slightly inhibits enzyme MGR II Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
1 * 36000, enzyme MGR I, 1 * 38000, enzyme MGR II, similar enzyme with NADPH-requirement for methylglyoxal reduction, that is inactive with NAD+, NADH and NADP+, SDS-PAGE Aspergillus niger
37000
-
enzyme MGR I, similar enzyme with NADPH-requirement for methylglyoxal reduction, that is inactive with NAD+, NADH and NADP+, gel filtration Aspergillus niger
38000
-
enzyme MGR II, similar enzyme with NADPH-requirement for methylglyoxal reduction, that is inactive with NAD+, NADH and NADP+, gel filtration Aspergillus niger

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methylglyoxal + NADPH Aspergillus niger similar enzyme with NADPH requirement lactaldehyde + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
2 methylglyoxylate reductases: MGR I and MGR II, similar enzyme with NADPH-requirement for methylglyoxal reduction, that is inactive with NAD+, NADH and NADP+
-

Purification (Commentary)

Purification (Comment) Organism
two methylglyoxylate reductases: MGR I and MGR II, similar enzyme with NADPH-requirement for methylglyoxal reduction, that is inactive with NAD+, NADH and NADP+ Aspergillus niger

Reaction

Reaction Comment Organism Reaction ID
(S)-lactaldehyde + NADP+ = 2-oxopropanal + NADPH + H+ similar enzyme with NADPH-requirement for methylglyoxal reduction, that is inactive with NAD+, NADH and NADP+ Aspergillus niger

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.83
-
enzyme MGR I Aspergillus niger
7.77
-
enzyme MGR II Aspergillus niger

Storage Stability

Storage Stability Organism
4°C Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + NADPH + H+ enzyme MGR II Aspergillus niger ethanol + NADP+
-
?
glyoxal + NADPH enzymes MGR I and MGR II Aspergillus niger glycolaldehyde + NADP+
-
?
methylglyoxal + NADPH similar enzyme with NADPH requirement Aspergillus niger lactaldehyde + NADP+
-
?
additional information enzyme MGR I: specific for 2-oxoaldehydes (glyoxal phenylglyoxal), enzyme MGR II: active towards 2-oxoaldehydes (glyoxal, methylglyoxal, phenylglyoxal), 4,5-dioxovalerate and some aldehydes (propionaldehyde and acetaldehyde) Aspergillus niger ?
-
?
phenylglyoxal + NADPH enzymes MGR I and MGR II Aspergillus niger hydroxyphenylacetaldehyde + NADP+
-
?
propionaldehyde + NADPH enzyme MGR II Aspergillus niger propanol + NADP+
-
?

Subunits

Subunits Comment Organism
monomer 1 * 36000, enzyme MGR I, 1 * 38000, enzyme MGR II, similar enzyme with NADPH-requirement for methylglyoxal reduction, that is inactive with NAD+, NADH and NADP+, SDS-PAGE Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
enzyme MGR I Aspergillus niger
9
-
enzyme MGR II Aspergillus niger

Cofactor

Cofactor Comment Organism Structure
NADPH NADPH required Aspergillus niger