Activating Compound | Comment | Organism | Structure |
---|---|---|---|
pyrazole | stimulates | Rattus norvegicus | |
pyrazole | no effect | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
folic acid | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
NAD+ | - |
Homo sapiens | |
0.0071 | - |
formaldehyde | - |
Rattus norvegicus | |
0.0087 | - |
formaldehyde | - |
Homo sapiens | |
0.01 | - |
NAD+ | - |
Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | contains metal | Homo sapiens | |
additional information | does not contain metal | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
90000 | - |
gel filtration | Homo sapiens |
110000 | - |
gel filtration | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.092 | - |
- |
Rattus norvegicus |
0.225 | - |
- |
Homo sapiens |
Storage Stability | Organism |
---|---|
0-5°C, when EDTA and dithiothreitol are used throughout the purification procedure, stable for at least 3 weeks | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formaldehyde + glutathione + NAD+ | - |
Homo sapiens | S-formylglutathione + NADH + H+ | - |
? | |
formaldehyde + glutathione + NAD+ | - |
Rattus norvegicus | S-formylglutathione + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme exists in a complex with other proteins or in a polymeric form until the ultimate steps in purification | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.4 | - |
Rattus norvegicus |
7.6 | 8.8 | - |
Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9.5 | pH 6.5: about 70% of maximal activity, pH 9.5: about 80% of maximal activity | Homo sapiens |
6.8 | 9.3 | pH 6.8: about 80% of maximal activity, pH 9.3: about 90% of maximal activity | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Homo sapiens | |
NAD+ | - |
Rattus norvegicus | |
NADP+ | no activity with NADP+ | Homo sapiens | |
NADP+ | no activity with NADP+ | Rattus norvegicus |