Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) cells | Branchiostoma floridae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
S-hydroxymethylglutathione | in 0.1 M sodium diphosphate at pH 8.0 | Branchiostoma floridae | |
0.008 | - |
NAD+ | in 0.1 M sodium diphosphate at pH 7.5 | Branchiostoma floridae | |
0.045 | - |
NADH | in 0.1 M sodium diphosphate at pH 7.5 | Branchiostoma floridae | |
0.056 | - |
S-nitrosoglutathione | in 0.1 M sodium diphosphate at pH 8.0 | Branchiostoma floridae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Branchiostoma floridae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Talon metal affinity resin chromatography | Branchiostoma floridae |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
gut | - |
Branchiostoma floridae | - |
additional information | dorsal region of the club-shaped gland | Branchiostoma floridae | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-hydroxymethylglutathione + NAD+ | - |
Branchiostoma floridae | S-formylglutathione + NADH + H+ | - |
? | |
S-nitrosoglutathione + NADH | - |
Branchiostoma floridae | GSH + NAD+ + NO | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADH3 | - |
Branchiostoma floridae |
alcohol dehydrogenase class III | - |
Branchiostoma floridae |
FALDH | - |
Branchiostoma floridae |
Glutathione-dependent formaldehyde dehydrogenase | - |
Branchiostoma floridae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.5 | - |
S-hydroxymethylglutathione | - |
Branchiostoma floridae | |
16.7 | - |
S-nitrosoglutathione | - |
Branchiostoma floridae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Branchiostoma floridae |