Crystallization (Comment) | Organism |
---|---|
- |
Gluconobacter oxydans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.032 | - |
NADPH | pH 6.0, 25°C | Gluconobacter oxydans | |
35 | - |
L-sorbose | pH 6.0, 25°C | Gluconobacter oxydans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Gluconobacter oxydans | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
2 * 30000, SDS-PAGE | Gluconobacter oxydans |
60000 | - |
gel filtration | Gluconobacter oxydans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gluconobacter oxydans | - |
- |
- |
Gluconobacter oxydans IFO 3294 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Gluconobacter oxydans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
77 | - |
pH 6.0, 25°C, substrate L-sorbose | Gluconobacter oxydans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-sorbitol + NADP+ | - |
Gluconobacter oxydans | L-sorbose + NADPH + H+ | - |
r | |
D-sorbitol + NADP+ | - |
Gluconobacter oxydans IFO 3294 | L-sorbose + NADPH + H+ | - |
r | |
L-sorbose + NADPH + H+ | - |
Gluconobacter oxydans | D-sorbitol + NADP+ | - |
r | |
L-sorbose + NADPH + H+ | - |
Gluconobacter oxydans IFO 3294 | D-sorbitol + NADP+ | - |
r | |
additional information | highly specific for D-sorbitol and L-sorbose. Reaction rate in L-sorbose reduction highly predominates over L-sorbitol oxidation over a wide pH range | Gluconobacter oxydans | ? | - |
? | |
additional information | highly specific for D-sorbitol and L-sorbose. Reaction rate in L-sorbose reduction highly predominates over L-sorbitol oxidation over a wide pH range | Gluconobacter oxydans IFO 3294 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 30000, SDS-PAGE | Gluconobacter oxydans |
Synonyms | Comment | Organism |
---|---|---|
L-sorbose reductase | - |
Gluconobacter oxydans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
reduction of L-sorbose | Gluconobacter oxydans |
8 | - |
oxidation of D-sorbitol | Gluconobacter oxydans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | reduction of L-sorbose | Gluconobacter oxydans |
7 | 9 | oxidation of D-sorbitol | Gluconobacter oxydans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no cofactor: NAD+, NADH | Gluconobacter oxydans | |
NADP+ | - |
Gluconobacter oxydans | |
NADPH | - |
Gluconobacter oxydans |