Literature summary for 1.1.1.289 extracted from

Kubota, K.; Nagata, K.; Miyazono, K.; Toyama, H.; Matsushita, K.; Tanokura, M.
Purification, crystallization and preliminary X-ray analysis of L-sorbose reductase from Gluconobacter frateurii complexed with L-sorbose or NADPH (2009), Acta Crystallogr. Sect. F, 65, 562-564.

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Cloned(Commentary)

Cloned (Comment)	Organism
into the HindIII/NotI site of pET-28a(+) plasmid, expression construct is designed to overexpress only L-sorbose reductase protein (residues 1-263), overexpressed in Escherichia coli BL21 (DE3) cells	Gluconobacter frateurii

Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with L-sorbose or NADPH using the sitting-drop vapour-diffusion method, at 20°C, to 2.38 and 1.90 A resolution, respectively. Crystal of the L-sorbose reductase-L-sorbose complex belongs to space group C2221, with unit-cell parameters a=124.2, b=124.1, c=60.8 A. The crystal of the L-sorbose reductase-NADPH complex belongs to space group P21, with unit-cell parameters a=124.3, b=61.0, c=124.5A, beta= 89.99°. The crystals contain two and eight molecules, respectively, in the asymmetric unit	Gluconobacter frateurii

Crystallization (Comment)

Organism

in complex with L-sorbose or NADPH using the sitting-drop vapour-diffusion method, at 20°C, to 2.38 and 1.90 A resolution, respectively. Crystal of the L-sorbose reductase-L-sorbose complex belongs to space group C2221, with unit-cell parameters a=124.2, b=124.1, c=60.8 A. The crystal of the L-sorbose reductase-NADPH complex belongs to space group P21, with unit-cell parameters a=124.3, b=61.0, c=124.5A, beta= 89.99°. The crystals contain two and eight molecules, respectively, in the asymmetric unit

Gluconobacter frateurii

Organism

Organism	UniProt	Comment	Textmining
Gluconobacter frateurii	A4PB64	-	-

Purification (Commentary)

Purification (Comment)	Organism
by sonication, centrifugation and gel filtration	Gluconobacter frateurii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-sorbose + NADPH + H+	-	Gluconobacter frateurii	L-sorbitol + NADP+	-	?

Synonyms

Synonyms	Comment	Organism
L-sorbose reductase	-	Gluconobacter frateurii

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	-	Gluconobacter frateurii

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Release 2023.1 (January 2023)