Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli Rosetta 2(DE3) | Metallosphaera sedula |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
NADPH | pH 7.4, 65°C | Metallosphaera sedula | |
0.07 | - |
malonate semialdehyde | pH 7.4, 65°C | Metallosphaera sedula |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | addition of Zn2+ (0-0.04 mM) or Mg2+or Mn2+(0-5 mM) to the enzyme assay mixture neither stimulates nor inactivates the enzyme | Metallosphaera sedula |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
3 * 33000, SDS-PAGE | Metallosphaera sedula |
105000 | - |
gel filtration | Metallosphaera sedula |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonate semialdehyde + NADPH + H+ | Metallosphaera sedula | the enzyme participates in the 3-hydroxypropionate/4-hydroxybutyrate cycle, an autotrophic CO2 fixation pathway found in some thermoacidophilic archaea | 3-hydroxypropanoate + NADP+ | - |
? | |
malonate semialdehyde + NADPH + H+ | Metallosphaera sedula DSM 5348 | the enzyme participates in the 3-hydroxypropionate/4-hydroxybutyrate cycle, an autotrophic CO2 fixation pathway found in some thermoacidophilic archaea | 3-hydroxypropanoate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Metallosphaera sedula | A4YI81 | - |
- |
Metallosphaera sedula DSM 5348 | A4YI81 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Metallosphaera sedula |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:autotrophically-grown cell | low activity in heterotrophically-grown cell | Metallosphaera sedula | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
200 | - |
pH 7.4, 65°C, heterologously expressed enzyme | Metallosphaera sedula |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonate semialdehyde + NADH + H+ | NADH can partially substitute (20% activity) for NADPH | Metallosphaera sedula | 3-hydroxypropanoate + NAD+ | - |
? | |
malonate semialdehyde + NADH + H+ | NADH can partially substitute (20% activity) for NADPH | Metallosphaera sedula DSM 5348 | 3-hydroxypropanoate + NAD+ | - |
? | |
malonate semialdehyde + NADPH + H+ | the enzyme participates in the 3-hydroxypropionate/4-hydroxybutyrate cycle, an autotrophic CO2 fixation pathway found in some thermoacidophilic archaea | Metallosphaera sedula | 3-hydroxypropanoate + NADP+ | - |
? | |
malonate semialdehyde + NADPH + H+ | NADH can partially substitute (20% activity) for NADPH | Metallosphaera sedula | 3-hydroxypropanoate + NADP+ | - |
? | |
malonate semialdehyde + NADPH + H+ | the enzyme participates in the 3-hydroxypropionate/4-hydroxybutyrate cycle, an autotrophic CO2 fixation pathway found in some thermoacidophilic archaea | Metallosphaera sedula DSM 5348 | 3-hydroxypropanoate + NADP+ | - |
? | |
malonate semialdehyde + NADPH + H+ | NADH can partially substitute (20% activity) for NADPH | Metallosphaera sedula DSM 5348 | 3-hydroxypropanoate + NADP+ | - |
? | |
additional information | succinic semialdehyde, acetaldehyde, butyraldehyde, propionaldehyde, or glutaraldehyde do not serve as a substrate | Metallosphaera sedula | ? | - |
? | |
additional information | succinic semialdehyde, acetaldehyde, butyraldehyde, propionaldehyde, or glutaraldehyde do not serve as a substrate | Metallosphaera sedula DSM 5348 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotrimer | 3 * 33000, SDS-PAGE | Metallosphaera sedula |
Synonyms | Comment | Organism |
---|---|---|
malonic semialdehyde reductase | - |
Metallosphaera sedula |
Msed_1993 | locus name | Metallosphaera sedula |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
assay at | Metallosphaera sedula |
75 | - |
- |
Metallosphaera sedula |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
115 | - |
NADPH | pH 7.4, 65°C | Metallosphaera sedula | |
115 | - |
malonate semialdehyde | pH 7.4, 65°C | Metallosphaera sedula |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.7 | - |
optimum pH at 65°C | Metallosphaera sedula |
7.4 | - |
asay at | Metallosphaera sedula |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | NADH can partially substitute (20% activity) for NADPH | Metallosphaera sedula | |
NADPH | NADH can partially substitute (20% activity) for NADPH | Metallosphaera sedula |
Organism | Comment | Expression |
---|---|---|
Metallosphaera sedula | the specific activity of this conversion in autotrophically grown cells is 1.8 microM/min * mg protein, whereas in heterotrophically grown cells the specific activity is 0.35 microM/min * mg protein | up |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme participates in the 3-hydroxypropionate/4-hydroxybutyrate cycle, an autotrophic CO2 fixation pathway found in some thermoacidophilic archaea | Metallosphaera sedula |