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Literature summary for 1.1.1.299 extracted from

  • Thompson, H.; Tersteegen, A.; Thauer, R.; Hedderich, R.
    Two malate dehydrogenases in Methanobacterium thermoautotrophicum (1998), Arch. Microbiol., 170, 38-42.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
NADH inhibitory above 0.2 mM Methanothermobacter thermautotrophicus
NADPH inhibitory above 0.2 mM Methanothermobacter thermautotrophicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.09
-
NADPH pH 7.6 Methanothermobacter thermautotrophicus
0.09
-
NADH pH 7.6 Methanothermobacter thermautotrophicus
0.09
-
oxaloacetate pH 7.6 Methanothermobacter thermautotrophicus
0.4
-
malate pH 7.6 Methanothermobacter thermautotrophicus
0.4
-
malate at pH 7.6 and 60°C Methanothermobacter thermautotrophicus
0.5
-
NAD+ pH 7.6 Methanothermobacter thermautotrophicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
x * 36000, SDS-PAGE, x * 36700, calculated Methanothermobacter thermautotrophicus
36700
-
x * 36000, SDS-PAGE, x * 36700, calculated Methanothermobacter thermautotrophicus

Organism

Organism UniProt Comment Textmining
Methanothermobacter thermautotrophicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Methanothermobacter thermautotrophicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1
-
pH 7.6, oxidation of malate, cosubstrate NAD+ Methanothermobacter thermautotrophicus
1
-
pH 7.6, oxidation of malate, cosubstrate NADP+ Methanothermobacter thermautotrophicus
52
-
pH 7.6, reduction of oxaloacetate, cosubstrate NADP+ Methanothermobacter thermautotrophicus
60
-
pH 7.6, reduction of oxaloacetate, cosubstrate NAD+ Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NAD+
-
Methanothermobacter thermautotrophicus oxaloacetate + NADH + H+
-
?
(S)-malate + NADP+
-
Methanothermobacter thermautotrophicus oxaloacetate + NADPH + H+
-
?
additional information no substrate: pyruvate Methanothermobacter thermautotrophicus ?
-
?
oxaloacetate + NADH
-
Methanothermobacter thermautotrophicus L-malate + NAD+
-
?
oxaloacetate + NADPH
-
Methanothermobacter thermautotrophicus L-malate + NADP+
-
?

Subunits

Subunits Comment Organism
? x * 36000, SDS-PAGE, x * 36700, calculated Methanothermobacter thermautotrophicus

Synonyms

Synonyms Comment Organism
MDH II uses both NADP+ and NAD+ Methanothermobacter thermautotrophicus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Methanothermobacter thermautotrophicus
NADH
-
Methanothermobacter thermautotrophicus
NADP+
-
Methanothermobacter thermautotrophicus
NADPH
-
Methanothermobacter thermautotrophicus