Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NADH | inhibitory above 0.2 mM | Methanothermobacter thermautotrophicus | |
NADPH | inhibitory above 0.2 mM | Methanothermobacter thermautotrophicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
NADPH | pH 7.6 | Methanothermobacter thermautotrophicus | |
0.09 | - |
NADH | pH 7.6 | Methanothermobacter thermautotrophicus | |
0.09 | - |
oxaloacetate | pH 7.6 | Methanothermobacter thermautotrophicus | |
0.4 | - |
malate | pH 7.6 | Methanothermobacter thermautotrophicus | |
0.4 | - |
malate | at pH 7.6 and 60°C | Methanothermobacter thermautotrophicus | |
0.5 | - |
NAD+ | pH 7.6 | Methanothermobacter thermautotrophicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
x * 36000, SDS-PAGE, x * 36700, calculated | Methanothermobacter thermautotrophicus |
36700 | - |
x * 36000, SDS-PAGE, x * 36700, calculated | Methanothermobacter thermautotrophicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanothermobacter thermautotrophicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanothermobacter thermautotrophicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1 | - |
pH 7.6, oxidation of malate, cosubstrate NAD+ | Methanothermobacter thermautotrophicus |
1 | - |
pH 7.6, oxidation of malate, cosubstrate NADP+ | Methanothermobacter thermautotrophicus |
52 | - |
pH 7.6, reduction of oxaloacetate, cosubstrate NADP+ | Methanothermobacter thermautotrophicus |
60 | - |
pH 7.6, reduction of oxaloacetate, cosubstrate NAD+ | Methanothermobacter thermautotrophicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | - |
Methanothermobacter thermautotrophicus | oxaloacetate + NADH + H+ | - |
? | |
(S)-malate + NADP+ | - |
Methanothermobacter thermautotrophicus | oxaloacetate + NADPH + H+ | - |
? | |
additional information | no substrate: pyruvate | Methanothermobacter thermautotrophicus | ? | - |
? | |
oxaloacetate + NADH | - |
Methanothermobacter thermautotrophicus | L-malate + NAD+ | - |
? | |
oxaloacetate + NADPH | - |
Methanothermobacter thermautotrophicus | L-malate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 36000, SDS-PAGE, x * 36700, calculated | Methanothermobacter thermautotrophicus |
Synonyms | Comment | Organism |
---|---|---|
MDH II | uses both NADP+ and NAD+ | Methanothermobacter thermautotrophicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Methanothermobacter thermautotrophicus | |
NADH | - |
Methanothermobacter thermautotrophicus | |
NADP+ | - |
Methanothermobacter thermautotrophicus | |
NADPH | - |
Methanothermobacter thermautotrophicus |