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Literature summary for 1.1.1.30 extracted from

  • Dalton, L.A.; McIntyre, J.O.; Fleischer, S.
    Effect of selective thiol-group derivatization on enzyme kinetics of (R)3-hydroxybutyrate dehydrogenase (1993), Biochem. J., 296, 563-569.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.30

Activating Compound

Activating Compound Comment Organism Structure
phosphatidylcholine
-
 Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.27
-
 NAD+ 240 mM Na+, purified enzyme, reconstituted with total mitochondrial phospholipids Bos taurus
2.2
-
 (R)-3-hydroxybutanoate 240 mM Na+, purified enzyme, reconstituted with total mitochondrial phospholipids Bos taurus
2.2
-
 (R)-3-hydroxybutanoate tetrameric enzyme (BDH-I) reconstituted with mitochondrial phospholipid vesicles, in the presence of 240 mM Na+, at pH 7.4 and 37°C Bos taurus
200
-
 (R)-3-hydroxybutanoate 240 mM Na+, purified enzyme, thiol group 1 modified with 1,1'-azobis(NN'-dimethylformamide), reconstituted with total mitochondrial phospholipids Bos taurus
200
-
 (R)-3-hydroxybutanoate enzyme form BDH-II, in the presence of 240 mM Na+, at pH 7.4 and 37°C Bos taurus
290
-
 (R)-3-hydroxybutanoate 240 mM Na+, purified enzyme, thiol group 2 modified with 1,1'-azobis(NN'-dimethylformamide), reconstituted with total mitochondrial phospholipids Bos taurus
290
-
 (R)-3-hydroxybutanoate enzyme form BDH-III, in the presence of 240 mM Na+, at pH 7.4 and 37°C Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Bos taurus 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-3-hydroxybutanoate + NAD+ Bos taurus
-
 acetoacetate + NADH
-
 r

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
heart
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-3-hydroxybutanoate + NAD+
-
 Bos taurus acetoacetate + NADH
-
 r
(R)-3-hydroxybutanoate + NAD+
-
 Bos taurus acetoacetate + NADH + H+
-
 r

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Bos taurus