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Literature summary for 1.1.1.30 extracted from

  • Uchino, K.; Saito, T.; Gebauer, B.; Jendrossek, D.
    Isolated poly(3-hydroxybutyrate) (PHB) granules are complex bacterial organelles catalyzing formation of PHB from acetyl coenzyme A (CoA) and degradation of PHB to acetyl-CoA (2007), J. Bacteriol., 189, 8250-8256.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
functional co-expression with acetoacetyl-CoA thiolase and PHB synthase in Escherichia coli, recombinant production of poly(3-hydroxybutanoate) granules, overview Cupriavidus necator

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-3-hydroxybutanoate + NADH + H+ Cupriavidus necator the enzyme is involved in poly(3-hydroxybutyrate) biosynthesis together with acetoacetyl-CoA thiolase and PHB synthase, pathway overview, intracellular concentrations of key metabolites, i.e. CoA, acetyl-CoA, 3HB-CoA, NAD+/NADH, determine whether a cell accumulates or degrades PHB acetoacetate + NAD+
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r

Organism

Organism UniProt Comment Textmining
Cupriavidus necator
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-
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information the enzyme is localized in poly(3-hydroxybutyrate) granules, co-localization with acetoacetyl-CoA thiolase and PHB synthase, overview Cupriavidus necator
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-3-hydroxybutanoate + NADH + H+
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Cupriavidus necator acetoacetate + NAD+
-
r
(R)-3-hydroxybutanoate + NADH + H+ the enzyme is involved in poly(3-hydroxybutyrate) biosynthesis together with acetoacetyl-CoA thiolase and PHB synthase, pathway overview, intracellular concentrations of key metabolites, i.e. CoA, acetyl-CoA, 3HB-CoA, NAD+/NADH, determine whether a cell accumulates or degrades PHB Cupriavidus necator acetoacetate + NAD+
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r

Synonyms

Synonyms Comment Organism
acetoacetyl-CoA reductase
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Cupriavidus necator

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Cupriavidus necator

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Cupriavidus necator

Cofactor

Cofactor Comment Organism Structure
additional information no activity with NADP+/NADPH Cupriavidus necator
NAD+
-
Cupriavidus necator
NADH
-
Cupriavidus necator