Cloned (Comment) | Organism |
---|---|
functional co-expression with acetoacetyl-CoA thiolase and PHB synthase in Escherichia coli, recombinant production of poly(3-hydroxybutanoate) granules, overview | Cupriavidus necator |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-3-hydroxybutanoate + NADH + H+ | Cupriavidus necator | the enzyme is involved in poly(3-hydroxybutyrate) biosynthesis together with acetoacetyl-CoA thiolase and PHB synthase, pathway overview, intracellular concentrations of key metabolites, i.e. CoA, acetyl-CoA, 3HB-CoA, NAD+/NADH, determine whether a cell accumulates or degrades PHB | acetoacetate + NAD+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cupriavidus necator | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | the enzyme is localized in poly(3-hydroxybutyrate) granules, co-localization with acetoacetyl-CoA thiolase and PHB synthase, overview | Cupriavidus necator | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-3-hydroxybutanoate + NADH + H+ | - |
Cupriavidus necator | acetoacetate + NAD+ | - |
r | |
(R)-3-hydroxybutanoate + NADH + H+ | the enzyme is involved in poly(3-hydroxybutyrate) biosynthesis together with acetoacetyl-CoA thiolase and PHB synthase, pathway overview, intracellular concentrations of key metabolites, i.e. CoA, acetyl-CoA, 3HB-CoA, NAD+/NADH, determine whether a cell accumulates or degrades PHB | Cupriavidus necator | acetoacetate + NAD+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
acetoacetyl-CoA reductase | - |
Cupriavidus necator |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Cupriavidus necator |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Cupriavidus necator |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with NADP+/NADPH | Cupriavidus necator | |
NAD+ | - |
Cupriavidus necator | |
NADH | - |
Cupriavidus necator |