Application | Comment | Organism |
---|---|---|
medicine | transfection with retinol dehydrogenase 12 protects cells against nonanal-induced toxicity but is ineffective against 4-hydroxynonenal. 4-Hydroxynonenal strongly inhibits the activities of lecithin:retinol acyl transferase and aldehyde dehydrogenase, resulting in decreased levels of retinyl esters and retinoic acid and accumulation of unesterified retinol | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression in HEK-293 cell | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
I51N | site-directed mutagenesis, significant activity in vitro. Dramatically reduced affinity for NADPH results in loss of function within cells | Homo sapiens |
L99I | site-directed mutagenesis, about 30% of wild-type activity | Homo sapiens |
additional information | transfection with retinol dehydrogenase 12 protects cells against nonanal-induced toxicity but is ineffective against 4-hydroxynonenal | Homo sapiens |
S175P | site-directed mutagenesis, no catalytic activity. Protein is stable and abundantly expressed | Homo sapiens |
T49M | site-directed mutagenesis, significant activity in vitro. Dramatically reduced affinity for NADPH results in loss of function within cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Nonanal | inhibits the activity towards retinaldehyde | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00006 | - |
all-trans-retinal | wild-type | Homo sapiens | |
0.00028 | - |
all-trans-retinal | mutant T49M | Homo sapiens | |
0.00041 | - |
all-trans-retinal | mutant I51N | Homo sapiens | |
0.0007 | - |
NADPH | wild-type | Homo sapiens | |
0.0032 | - |
NADP+ | wild-type | Homo sapiens | |
0.033 | - |
NADPH | mutant T49M | Homo sapiens | |
0.1 | - |
NADP+ | mutant T49M | Homo sapiens | |
0.105 | - |
NADPH | mutant I51N | Homo sapiens | |
0.35 | - |
NADP+ | mutant I51N | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
all-trans-retinal + NADPH + H+ | Homo sapiens | - |
all-trans-retinol + NADP+ | - |
? | |
additional information | Homo sapiens | although bi-directional in vitro, in living cells, RDH12 acts exclusively as a retinaldehyde reductase, shifting the retinoid homeostasis toward the increased levels of retinol and decreased levels of bioactive retinoic acid. The retinaldehyde reductase activity of RDH12 protects the cells from retinaldehyde-induced cell death | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
isoform RDH12 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
all-trans-retinal + NADPH + H+ | - |
Homo sapiens | all-trans-retinol + NADP+ | - |
? | |
additional information | although bi-directional in vitro, in living cells, RDH12 acts exclusively as a retinaldehyde reductase, shifting the retinoid homeostasis toward the increased levels of retinol and decreased levels of bioactive retinoic acid. The retinaldehyde reductase activity of RDH12 protects the cells from retinaldehyde-induced cell death | Homo sapiens | ? | - |
? | |
retinal + NADH | - |
Homo sapiens | retinol + NAD+ | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
all-trans-retinal | wild-type | Homo sapiens | |
0.21 | - |
all-trans-retinal | mutant I51N | Homo sapiens | |
0.4 | - |
all-trans-retinal | mutant T49M | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Homo sapiens | |
NADPH | - |
Homo sapiens |