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Literature summary for 1.1.1.300 extracted from

  • Haller, F.; Moman, E.; Hartmann, R.W.; Adamski, J.; Mindnich, R.
    Molecular framework of steroid/retinoid discrimination in 17beta-hydroxysteroid dehydrogenase type 1 and photoreceptor-associated retinol dehydrogenase (2010), J. Mol. Biol., 399, 255-267.
    View publication on PubMed
Search for more literature for 1.1.1.300

Cloned(Commentary)

Cloned (Comment) Organism
expression in HEK-293 cell Danio rerio
expression in HEK-293 cell Homo sapiens

Protein Variants

Protein Variants Comment Organism
M144G gain-of-function mutant, enables estrone to bind and be reduced as an additional substrate Homo sapiens
M146G mutation in isoform prRDH1, gain-of-function mutant, enables estrone to bind and be reduced as an additional substrate Danio rerio
M147G mutation in isoform prRDH2, gain-of-function mutant, enables estrone to bind and be reduced as an additional substrate Danio rerio

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00126
-
 all-trans-retinal wild-type, pH 7.3, 37°C Homo sapiens
0.0017
-
 all-trans-retinal mutant M146G, pH 7.3, 37°C Danio rerio
0.0023
-
 all-trans-retinal mutant M144G, pH 7.3, 37°C Homo sapiens
0.003
-
 all-trans-retinal mutant M147G, pH 7.3, 37°C Danio rerio
0.0031
-
 all-trans-retinal wild-type isoform prRDH1, pH 7.3, 37°C Danio rerio
0.0044
-
 all-trans-retinal wild-type isoform prRDH1, pH 7.3, 37°C Danio rerio
0.0096
-
 estrone mutant M144G, pH 7.3, 37°C Homo sapiens
0.0233
-
 estrone mutant M147G, pH 7.3, 37°C Danio rerio
0.0307
-
 estrone mutant M146G, pH 7.3, 37°C Danio rerio

Organism

Organism UniProt Comment Textmining
Danio rerio
-
-
-
Homo sapiens Q9NYR8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
all-trans-retinal + NADH + H+
-
 Danio rerio all-trans-retinol + NAD+
-
 ?
all-trans-retinal + NADH + H+
-
 Homo sapiens all-trans-retinol + NAD+
-
 ?
estrone + NADH + H+ no substrate for wild-type isoforms prRDH1 and prRDH2, but substrate for mutants M146G of prRDH1 and M147G of prRDH2 Danio rerio estradiol + NAD+
-
 ?
estrone + NADH + H+ no substrate for wild-type, but substrate for mutant M144G Homo sapiens estradiol + NAD+
-
 ?

Synonyms

Synonyms Comment Organism
photoreceptor-associated retinol dehydrogenase
-
 Danio rerio
photoreceptor-associated retinol dehydrogenase
-
 Homo sapiens
prRDH
-
 Danio rerio
prRDH
-
 Homo sapiens