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Literature summary for 1.1.1.300 extracted from

  • Hong, S.H.; Ngo, H.P.; Nam, H.K.; Kim, K.R.; Kang, L.W.; Oh, D.K.
    Alternative biotransformation of retinal to retinoic acid or retinol by an aldehyde dehydrogenase from Bacillus cereus (2016), Appl. Environ. Microbiol., 82, 3940-3946.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.300

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli ER2566 cells Bacillus cereus

Protein Variants

Protein Variants Comment Organism
C300A the mutant shows no oxidation activity Bacillus cereus
C300S the mutant shows reduced catalytic efficiency for the oxidation and reduction of all-trans-retinal compared to the wild-type enzyme Bacillus cereus
E194S the mutant shows 15fold higher catalytic efficiency for the reduction of all-trans-retinal than the wild-type enzyme Bacillus cereus
E266A the mutant shows no oxidation activity Bacillus cereus
E457V the mutant shows 7.5fold higher catalytic efficiency for the reduction of all-trans-retinal than the wild-type enzyme Bacillus cereus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
 NAD+ at pH 7.0 and 37°C Bacillus cereus
0.0042
-
 NADP+ at pH 7.0 and 37°C Bacillus cereus
0.007
-
 retinal with NAD+ as cosubstrate, at pH 7.0 and 37°C Bacillus cereus
0.04
-
 NADPH at pH 7.0 and 37°C Bacillus cereus
0.13
-
 retinal with NADPH as cosubstrate, at pH 7.0 and 37°C Bacillus cereus

Organism

Organism UniProt Comment Textmining
Bacillus cereus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Sephacryl S-300 gel filtration Bacillus cereus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.012
-
 mutant enzyme C300S, with retinal and NADPH as substrates, at pH 7.0 and 37°C Bacillus cereus
0.0386
-
 mutant enzyme C300S, with retinal and NAD+ as substrates, at pH 7.0 and 37°C Bacillus cereus
0.068
-
 mutant enzyme C300A, with retinal and NADPH as substrates, at pH 7.0 and 37°C Bacillus cereus
0.08
-
 mutant enzyme E266A, with retinal and NADPH as substrates, at pH 7.0 and 37°C Bacillus cereus
0.136
-
 wild type enzyme, with retinal and NADPH as substrates, at pH 7.0 and 37°C Bacillus cereus
0.26
-
 mutant enzyme E457V, with retinal and NADPH as substrates, at pH 7.0 and 37°C Bacillus cereus
0.408
-
 mutant enzyme E194S, with retinal and NADPH as substrates, at pH 7.0 and 37°C Bacillus cereus
0.461
-
 wild type enzyme, with retinal and NAD+ as substrates, at pH 7.0 and 37°C Bacillus cereus
0.493
-
 mutant enzyme E194S, with retinal and NAD+ as substrates, at pH 7.0 and 37°C Bacillus cereus
2.536
-
 mutant enzyme E457V, with retinal and NAD+ as substrates, at pH 7.0 and 37°C Bacillus cereus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with 9-cis-retinal and 13-cis-retinal Bacillus cereus ?
-
 ?
retinal + NAD+
-
 Bacillus cereus retinoic acid + NADH + H+
-
 ?
retinal + NADPH + H+
-
 Bacillus cereus retinol + NADP+
-
 ?
retinol + NADP+
-
 Bacillus cereus retinal + NADPH + H+
-
 ?

Synonyms

Synonyms Comment Organism
aldehyde dehydrogenase
-
 Bacillus cereus
ALDH
-
 Bacillus cereus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00055
-
 NADPH at pH 7.0 and 37°C Bacillus cereus
0.0017
-
 NAD+ at pH 7.0 and 37°C Bacillus cereus
0.0018
-
 NADP+ at pH 7.0 and 37°C Bacillus cereus
0.002
-
 retinal with NADPH as cosubstrate, at pH 7.0 and 37°C Bacillus cereus
0.0047
-
 retinal with NAD+ as cosubstrate, at pH 7.0 and 37°C Bacillus cereus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Bacillus cereus
NADH
-
 Bacillus cereus
NADP+
-
 Bacillus cereus
NADPH
-
 Bacillus cereus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.014
-
 NADPH at pH 7.0 and 37°C Bacillus cereus
0.015
-
 retinal with NADPH as cosubstrate, at pH 7.0 and 37°C Bacillus cereus
0.417
-
 NAD+ at pH 7.0 and 37°C Bacillus cereus
0.433
-
 NADP+ at pH 7.0 and 37°C Bacillus cereus
0.667
-
 retinal with NAD+ as cosubstrate, at pH 7.0 and 37°C Bacillus cereus