Literature summary for 1.1.1.31 extracted from

Rougraff, P.M.; Paxton, R.; Kuntz, M.J.; Crabb, D.W.; Harris, R.A
Purification and characterization of 3-hydroxyisobutyrate dehydrogenase from rat liver (1988), J. Biol. Chem., 263, 327-331.

Search for more literature for 1.1.1.31

Inhibitors

Inhibitors	Comment	Organism	Structure
(R)-3-hydroxy-2-methylpropanoate	competitive inhibitor of the S-isomer	Oryctolagus cuniculus
NADH	product inhibition	Oryctolagus cuniculus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
34000	-	alpha2, 2 * 34000, SDS-PAGE	Oryctolagus cuniculus
74000	-	gel filtration	Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-3-hydroxyisobutyrate + NAD+	Oryctolagus cuniculus	-	L-2-methyl-3-oxopropionate + NADH	-	r

Organism

Organism	UniProt	Comment	Textmining
Oryctolagus cuniculus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Oryctolagus cuniculus

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Oryctolagus cuniculus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
10.7	-	-	Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(R)-3-hydroxyisobutyrate + NAD+	-	Oryctolagus cuniculus	methylmalonate semialdehyde + NADH + H+	-	r
L-3-hydroxyisobutyrate + NAD+	-	Oryctolagus cuniculus	L-2-methyl-3-oxopropionate + NADH	-	r

Subunits

Subunits	Comment	Organism
homodimer	alpha2, 2 * 34000, SDS-PAGE	Oryctolagus cuniculus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.49	-	(R)-3-hydroxyisobutyrate	-	Oryctolagus cuniculus
7.1	-	(S)-3-hydroxyisobutyrate	-	Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9	11	-	Oryctolagus cuniculus

pH Range

pH Minimum	pH Maximum	Comment	Organism
7	11.5	-	Oryctolagus cuniculus

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Oryctolagus cuniculus

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Release 2023.1 (January 2023)