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Literature summary for 1.1.1.31 extracted from

  • Zhou, S.; Mohan Raj, S.; Ashok, S.; Edwardraja, S.; Lee, S.G.; Park, S.
    Cloning, expression and characterization of 3-hydroxyisobutyrate dehydrogenase from Pseudomonas denitrificans ATCC 13867 (2013), PLoS ONE, 8, e62666.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.31

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol about 182.8% activity at 1 mM Pseudomonas denitrificans (nom. rej.)
dithiothreitol about 184.8% activity at 1 mM Pseudomonas denitrificans (nom. rej.)
EDTA about 150.9% activity at 1 mM Pseudomonas denitrificans (nom. rej.)

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Pseudomonas denitrificans (nom. rej.)

Inhibitors

Inhibitors Comment Organism Structure
Ag+ complete inhibition at 1 mM Pseudomonas denitrificans (nom. rej.)
Co2+ about 64.3% residual activity at 1 mM Pseudomonas denitrificans (nom. rej.)
Cu2+ about 14.5% residual activity at 1 mM Pseudomonas denitrificans (nom. rej.)
Fe2+ about 9.1% residual activity at 1 mM Pseudomonas denitrificans (nom. rej.)
Hg2+ complete inhibition at 1 mM Pseudomonas denitrificans (nom. rej.)
Mg2+ about 88.6% residual activity at 1 mM Pseudomonas denitrificans (nom. rej.)
Ni2+ about 62.3% residual activity at 1 mM Pseudomonas denitrificans (nom. rej.)
Zn2+ about 14.5% residual activity at 1 mM Pseudomonas denitrificans (nom. rej.)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.164
-
 NAD+ with 3-hydroxypropionic acid as cosubstrate, at 30°C and pH 9.0 Pseudomonas denitrificans (nom. rej.)
0.178
-
 (S)-3-hydroxyisobutyrate at 30°C and pH 9.0 Pseudomonas denitrificans (nom. rej.)
1.055
-
 3-hydroxypropionic acid at 30°C and pH 9.0 Pseudomonas denitrificans (nom. rej.)
24.18
-
 methyl DL-serine at 30°C and pH 9.0 Pseudomonas denitrificans (nom. rej.)
25.14
-
 methyl (2S)-3-hydroxy-2-methylpropanoate at 30°C and pH 9.0 Pseudomonas denitrificans (nom. rej.)
117.5
-
 L-serine at 30°C and pH 9.0 Pseudomonas denitrificans (nom. rej.)

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ about 130.7% activity at 1 mM Pseudomonas denitrificans (nom. rej.)
additional information not influenced by Na+, K+, NH3+, and Li+ Pseudomonas denitrificans (nom. rej.)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 x * 30000, SDS-PAGE Pseudomonas denitrificans (nom. rej.)

Organism

Organism UniProt Comment Textmining
Pseudomonas denitrificans (nom. rej.)
-
-
-
Pseudomonas denitrificans (nom. rej.) ATCC 13867
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography Pseudomonas denitrificans (nom. rej.)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-3-hydroxyisobutyrate + NAD+ the enzyme is very specific to (S)-3-hydroxyisobutyrate Pseudomonas denitrificans (nom. rej.) 2-methyl-3-oxopropanoate + NADH + H+
-
 ?
(S)-3-hydroxyisobutyrate + NAD+ the enzyme is very specific to (S)-3-hydroxyisobutyrate Pseudomonas denitrificans (nom. rej.) ATCC 13867 2-methyl-3-oxopropanoate + NADH + H+
-
 ?
3-hydroxypropionic acid + NAD+
-
 Pseudomonas denitrificans (nom. rej.) malonate semialdehyde + NADH + H+
-
 r
3-hydroxypropionic acid + NAD+
-
 Pseudomonas denitrificans (nom. rej.) ATCC 13867 malonate semialdehyde + NADH + H+
-
 r
L-serine + NAD+
-
 Pseudomonas denitrificans (nom. rej.) 3-oxo-L-alanine + NADH + H+
-
 r
L-serine + NAD+
-
 Pseudomonas denitrificans (nom. rej.) ATCC 13867 3-oxo-L-alanine + NADH + H+
-
 r
methyl (2S)-3-hydroxy-2-methylpropanoate + NAD+
-
 Pseudomonas denitrificans (nom. rej.) methyl (2S)-2-methyl-3-oxopropanoate + NADH + H+
-
 r
methyl (2S)-3-hydroxy-2-methylpropanoate + NAD+
-
 Pseudomonas denitrificans (nom. rej.) ATCC 13867 methyl (2S)-2-methyl-3-oxopropanoate + NADH + H+
-
 r
methyl DL-serine + NAD+
-
 Pseudomonas denitrificans (nom. rej.) methyl 3-oxo-DL-alanine + NADH + H+
-
 r
methyl DL-serine + NAD+
-
 Pseudomonas denitrificans (nom. rej.) ATCC 13867 methyl 3-oxo-DL-alanine + NADH + H+
-
 r

Subunits

Subunits Comment Organism
? x * 30000, SDS-PAGE Pseudomonas denitrificans (nom. rej.)

Synonyms

Synonyms Comment Organism
3HIBDH-IV
-
 Pseudomonas denitrificans (nom. rej.)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Pseudomonas denitrificans (nom. rej.)

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 40 more than 80% activity between 25°C and 40°C Pseudomonas denitrificans (nom. rej.)

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.62
-
 methyl DL-serine at 30°C and pH 9.0 Pseudomonas denitrificans (nom. rej.)
6.717
-
 NAD+ with 3-hydroxypropionic acid as cosubstrate, at 30°C and pH 9.0 Pseudomonas denitrificans (nom. rej.)
10.8
-
 3-hydroxypropionic acid at 30°C and pH 9.0 Pseudomonas denitrificans (nom. rej.)
35.76
-
 methyl (2S)-3-hydroxy-2-methylpropanoate at 30°C and pH 9.0 Pseudomonas denitrificans (nom. rej.)
47.55
-
 L-serine at 30°C and pH 9.0 Pseudomonas denitrificans (nom. rej.)
80.79
-
 (S)-3-hydroxyisobutyrate at 30°C and pH 9.0 Pseudomonas denitrificans (nom. rej.)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Pseudomonas denitrificans (nom. rej.)

pH Range

pH Minimum pH Maximum Comment Organism
8 10 more than 60% activity between pH 8.0 and 10.0 Pseudomonas denitrificans (nom. rej.)

Cofactor

Cofactor Comment Organism Structure
NAD+ dependent on Pseudomonas denitrificans (nom. rej.)

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.275
-
 methyl DL-serine at 30°C and pH 9.0 Pseudomonas denitrificans (nom. rej.)
0.404
-
 L-serine at 30°C and pH 9.0 Pseudomonas denitrificans (nom. rej.)
1.433
-
 methyl (2S)-3-hydroxy-2-methylpropanoate at 30°C and pH 9.0 Pseudomonas denitrificans (nom. rej.)
10.23
-
 3-hydroxypropionic acid at 30°C and pH 9.0 Pseudomonas denitrificans (nom. rej.)
42.44
-
 NAD+ with 3-hydroxypropionic acid as cosubstrate, at 30°C and pH 9.0 Pseudomonas denitrificans (nom. rej.)
464.9
-
 (S)-3-hydroxyisobutyrate at 30°C and pH 9.0 Pseudomonas denitrificans (nom. rej.)