Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(R)-1-phenylethanol | oxidation of (S)-1-phenylethanol is completely inhibited in the presence of tenfold higher concentrations of (R)-1-phenylethanol | Azoarcus sp. | |
additional information | the presence of 10 mM EDTA (10 mM), 1 mM pyrazole or 1 mM 1,10-phenanthroline in enzyme assays does not affect the activity, indicating that the enzyme does not contain easily accessible bivalent metal ions. (S)-1-phenylethanol oxidation is not inhibited by 0.1 mM 5,5'-dithiobis-(2-nitrobenzoic acid), 1 mM 4-hydroxymercuribenzoic acid or 1 mM N-ethylmaleimide, indicating that no accessible cysteine residues are required for catalysis | Azoarcus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
acetophenone | pH 8, 30°C | Azoarcus sp. | |
0.0016 | - |
(S)-1-phenylethanol | pH 8, 30°C | Azoarcus sp. | |
0.0033 | - |
NADH | pH 8, 30°C | Azoarcus sp. | |
0.051 | - |
NAD+ | pH 8, 30°C | Azoarcus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25500 | - |
2 * 25500, SDS-PAGE | Azoarcus sp. |
50000 | - |
gel filtration | Azoarcus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-1-phenylethanol + NAD+ | Azoarcus sp. | - |
acetophenone + NADH + H+ | - |
r | |
(S)-1-phenylethanol + NAD+ | Azoarcus sp. EbN1 | - |
acetophenone + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Azoarcus sp. | - |
- |
- |
Azoarcus sp. EbN1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Azoarcus sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:ethylbenzene-grown cell | - |
Azoarcus sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.8 | - |
pH 8, 30°C | Azoarcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-1-phenylethanol + NAD+ | - |
Azoarcus sp. | acetophenone + NADH + H+ | - |
r | |
(S)-1-phenylethanol + NAD+ | no activity with NADP+. No activity with (R)-1-phenylethanol | Azoarcus sp. | acetophenone + NADH + H+ | - |
r | |
(S)-1-phenylethanol + NAD+ | - |
Azoarcus sp. EbN1 | acetophenone + NADH + H+ | - |
r | |
(S)-1-phenylethanol + NAD+ | no activity with NADP+. No activity with (R)-1-phenylethanol | Azoarcus sp. EbN1 | acetophenone + NADH + H+ | - |
r | |
additional information | no activity with ethanol, 1-propanol, 1-butanol, 2-phenylethanol or benzyl alcohol | Azoarcus sp. | ? | - |
? | |
additional information | no activity with ethanol, 1-propanol, 1-butanol, 2-phenylethanol or benzyl alcohol | Azoarcus sp. EbN1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 25500, SDS-PAGE | Azoarcus sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.6 | - |
NADH | pH 8, 30°C | Azoarcus sp. | |
1.6 | - |
acetophenone | pH 8, 30°C | Azoarcus sp. | |
2 | - |
NAD+ | pH 8, 30°C | Azoarcus sp. | |
2 | - |
(S)-1-phenylethanol | pH 8, 30°C | Azoarcus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
reduction of acetophenone | Azoarcus sp. |
8 | - |
oxidation of (S)-1-phenylethanol | Azoarcus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | no activity with NADP+ | Azoarcus sp. | |
NADH | - |
Azoarcus sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Azoarcus sp. | two-dimensional gel electrophoresis | - |
6.6 |