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Literature summary for 1.1.1.311 extracted from

  • Kniemeyer, O.; Heider, J.
    (S)-1-Phenylethanol dehydrogenase of Azoarcus sp. strain EbN1, an enzyme of anaerobic ethylbenzene catabolism (2001), Arch. Microbiol., 176, 129-135.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
(R)-1-phenylethanol oxidation of (S)-1-phenylethanol is completely inhibited in the presence of tenfold higher concentrations of (R)-1-phenylethanol Azoarcus sp.
additional information the presence of 10 mM EDTA (10 mM), 1 mM pyrazole or 1 mM 1,10-phenanthroline in enzyme assays does not affect the activity, indicating that the enzyme does not contain easily accessible bivalent metal ions. (S)-1-phenylethanol oxidation is not inhibited by 0.1 mM 5,5'-dithiobis-(2-nitrobenzoic acid), 1 mM 4-hydroxymercuribenzoic acid or 1 mM N-ethylmaleimide, indicating that no accessible cysteine residues are required for catalysis Azoarcus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
acetophenone pH 8, 30°C Azoarcus sp.
0.0016
-
(S)-1-phenylethanol pH 8, 30°C Azoarcus sp.
0.0033
-
NADH pH 8, 30°C Azoarcus sp.
0.051
-
NAD+ pH 8, 30°C Azoarcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25500
-
2 * 25500, SDS-PAGE Azoarcus sp.
50000
-
gel filtration Azoarcus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-1-phenylethanol + NAD+ Azoarcus sp.
-
acetophenone + NADH + H+
-
r
(S)-1-phenylethanol + NAD+ Azoarcus sp. EbN1
-
acetophenone + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Azoarcus sp.
-
-
-
Azoarcus sp. EbN1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Azoarcus sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:ethylbenzene-grown cell
-
Azoarcus sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.8
-
pH 8, 30°C Azoarcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-1-phenylethanol + NAD+
-
Azoarcus sp. acetophenone + NADH + H+
-
r
(S)-1-phenylethanol + NAD+ no activity with NADP+. No activity with (R)-1-phenylethanol Azoarcus sp. acetophenone + NADH + H+
-
r
(S)-1-phenylethanol + NAD+
-
Azoarcus sp. EbN1 acetophenone + NADH + H+
-
r
(S)-1-phenylethanol + NAD+ no activity with NADP+. No activity with (R)-1-phenylethanol Azoarcus sp. EbN1 acetophenone + NADH + H+
-
r
additional information no activity with ethanol, 1-propanol, 1-butanol, 2-phenylethanol or benzyl alcohol Azoarcus sp. ?
-
?
additional information no activity with ethanol, 1-propanol, 1-butanol, 2-phenylethanol or benzyl alcohol Azoarcus sp. EbN1 ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 25500, SDS-PAGE Azoarcus sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.6
-
NADH pH 8, 30°C Azoarcus sp.
1.6
-
acetophenone pH 8, 30°C Azoarcus sp.
2
-
NAD+ pH 8, 30°C Azoarcus sp.
2
-
(S)-1-phenylethanol pH 8, 30°C Azoarcus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
reduction of acetophenone Azoarcus sp.
8
-
oxidation of (S)-1-phenylethanol Azoarcus sp.

Cofactor

Cofactor Comment Organism Structure
NAD+ no activity with NADP+ Azoarcus sp.
NADH
-
Azoarcus sp.

pI Value

Organism Comment pI Value Maximum pI Value
Azoarcus sp. two-dimensional gel electrophoresis
-
6.6