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Literature summary for 1.1.1.312 extracted from

  • Masai, E.; Momose, K.; Hara, H.; Nisihikawa, S.; Katayama, Y.; Fukuda, M.
    Genetic and biochemical characterization of 4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase and its role in the protocatechuate 4,5-cleavage pathway in Sphingomonas paucimobilis SYK-6 (2000), J. Bacteriol., 182, 6651-6658.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase gene ligC expressed in Escherichia coli Sphingomonas paucimobilis

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoate) 1 h at 0.1 mM inhibits 98% of enzyme activity Sphingomonas paucimobilis
HgCl2 1 h at 0.01 mM inhibits 83% of enzyme activity Sphingomonas paucimobilis
p-chloromercuribenzoate 1 h at 0.01 mM inhibits 78% of enzyme activity Sphingomonas paucimobilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0206
-
 4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde in the presence of NAD+ Sphingomonas paucimobilis
0.0246
-
 NADP+
-
 Sphingomonas paucimobilis
0.026
-
 4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde in the presence of NADP+ Sphingomonas paucimobilis
0.252
-
 NAD+
-
 Sphingomonas paucimobilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34590
-
 1 * 34590, amino acid sequence Sphingomonas paucimobilis
67000
-
 gel filtration Sphingomonas paucimobilis

Organism

Organism UniProt Comment Textmining
Sphingomonas paucimobilis
-
 SYK6
-
Sphingomonas paucimobilis SYK6
-
 SYK6
-

Purification (Commentary)

Purification (Comment) Organism
from the cell extract of Escherichia coli, using POROS polyethyleneimine anion-exchange chromatography, POROS quaternized PI anion-exchange chromatography and POROS phenylether hydrophobic-interaction chromatography Sphingomonas paucimobilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
360
-
 after purification from the cell extract of Escherichia coli Sphingomonas paucimobilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde + NAD+
-
 Sphingomonas paucimobilis 4-carboxy-2-hydroxy-cis,cis-muconate + NADH
-
 ?
4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde + NAD+
-
 Sphingomonas paucimobilis SYK6 4-carboxy-2-hydroxy-cis,cis-muconate + NADH
-
 ?
4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde + NADP+
-
 Sphingomonas paucimobilis 4-carboxy-2-hydroxy-cis,cis-muconate + NADPH following intramolecular dehydrogenation to the lactone 2-pyrone-4,6-dicarboxylic acid ?
4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde + NADP+
-
 Sphingomonas paucimobilis SYK6 4-carboxy-2-hydroxy-cis,cis-muconate + NADPH following intramolecular dehydrogenation to the lactone 2-pyrone-4,6-dicarboxylic acid ?

Subunits

Subunits Comment Organism
dimer 1 * 34590, amino acid sequence Sphingomonas paucimobilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
 Sphingomonas paucimobilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Sphingomonas paucimobilis

Cofactor

Cofactor Comment Organism Structure
NAD+ can replace NADP+, but with lower affinity Sphingomonas paucimobilis
NADP+
-
 Sphingomonas paucimobilis