Application | Comment | Organism |
---|---|---|
synthesis | asymmetric reduction with Bacillus cereus benzil reductase can be utilized to produce important chiral compounds | Bacillus cereus |
Cloned (Comment) | Organism |
---|---|
expression of GFP-tagged enzyme in Bacillus cereus strain IFO3563, subcloning in Escherichia coli | Bacillus cereus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.042 | - |
1-phenyl-1,2-propanedione | recombinant enzyme, pH 6.5, 37°C | Bacillus cereus | |
0.768 | - |
benzil | recombinant enzyme, pH 6.5, 37°C | Bacillus cereus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-benzoin + NADP+ | Bacillus cereus | stereospecific asymmetric reduction of benzil to (S)-benzoin | benzil + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | - |
- |
- |
Bacillus cereus | - |
diverse strains, overview | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain DH5alpha by ammonium sulfate fractionation | Bacillus cereus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-benzoin + NADP+ | stereospecific asymmetric reduction of benzil to (S)-benzoin | Bacillus cereus | benzil + NADPH + H+ | - |
r | |
(S)-benzoin + NADP+ | stereospecific asymmetric reduction of benzil to (S)-benzoin, recombinant Bacillus cereus benzil reductase produces optically pure (S)-benzoin with NADPH in vitro | Bacillus cereus | benzil + NADPH + H+ | - |
r | |
1-(4-fluoro-phenyl)-2-phenyl-ethane-1,2-dione + NADPH + H+ | - |
Bacillus cereus | ? + NADP+ | - |
? | |
1-(4-methyl-phenyl)-2-phenyl-ethane-1,2-dione + NADPH + H+ | - |
Bacillus cereus | ? + NADP+ | - |
? | |
1-phenyl-1,2-propanedione + NADPH + H+ | - |
Bacillus cereus | ? + NADP+ | - |
? | |
2-hydroxy-1-phenyl-1-propanone + NADP+ | - |
Bacillus cereus | 1-phenylpropane-1,2-dione + NADPH + H+ | - |
? | |
additional information | the enzyme is also active with 1,4-naphthoquinone, dichlone, and 4-phenylbenzaldehyde, substrate specificity, overview. 1-acenaphthenol is oxidized, although the kcat/Km ratio is low. No activity with progesterone, daunorubicin, and menaquinone. No or poor activity with benzyl phenyl ketone, benzophenone, dibenzoylmethane, chalcone, 1-phenyl-1,3-butanedione, diacetyl, 3,4-hexanedione, and acetophenone | Bacillus cereus | ? | - |
? | |
sec-phenethyl alcohol + NADP+ | - |
Bacillus cereus | 1-phenylethanone + NADPH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
benzil reductase | - |
Bacillus cereus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bacillus cereus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.07 | - |
benzil | recombinant enzyme, pH 6.5, 37°C | Bacillus cereus | |
2.75 | - |
1-phenyl-1,2-propanedione | recombinant enzyme, pH 6.5, 37°C | Bacillus cereus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at, reduction reaction | Bacillus cereus |
10 | - |
assay at, oxidation reaction | Bacillus cereus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Bacillus cereus | |
NADPH | - |
Bacillus cereus |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is a member of the short-chain dehydrogenase/reductase, SDR, family | Bacillus cereus |
physiological function | amino acid differences in the benzil reductase among Bacillus cereus strains | Bacillus cereus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.4 | - |
benzil | recombinant enzyme, pH 6.5, 37°C | Bacillus cereus | |
65.33 | - |
1-phenyl-1,2-propanedione | recombinant enzyme, pH 6.5, 37°C | Bacillus cereus |