Application | Comment | Organism |
---|---|---|
pharmacology | zerumbone is a predominating potential multi-anticancer agent | Zingiber zerumbet |
Cloned (Comment) | Organism |
---|---|
gene ZSD1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Zingiber zerumbet |
Protein Variants | Comment | Organism |
---|---|---|
K159A | site-directed mutagenesis, inactive mutant | Zingiber zerumbet |
S142A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Zingiber zerumbet |
S144A | site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme | Zingiber zerumbet |
Y155A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Zingiber zerumbet |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics with borneol as substrate | Zingiber zerumbet | |
0.0161 | - |
NAD+ | recombinant mutant S144A, pH 8.0, 37°C | Zingiber zerumbet | |
0.0273 | - |
NAD+ | recombinant wild-type enzyme, pH 8.0, 37°C | Zingiber zerumbet | |
0.028 | - |
10-hydroxy-alpha-humulene | recombinant mutant S144A, pH 8.0, 37°C | Zingiber zerumbet | |
0.0437 | - |
NAD+ | recombinant mutant Y155A, pH 8.0, 37°C | Zingiber zerumbet | |
0.0585 | - |
10-hydroxy-alpha-humulene | recombinant wild-type enzyme, pH 8.0, 37°C | Zingiber zerumbet | |
0.0782 | - |
10-hydroxy-alpha-humulene | recombinant mutant Y155A, pH 8.0, 37°C | Zingiber zerumbet | |
0.121 | - |
NAD+ | recombinant mutant S142A, pH 8.0, 37°C | Zingiber zerumbet | |
0.138 | - |
10-hydroxy-alpha-humulene | recombinant mutant S142A, pH 8.0, 37°C | Zingiber zerumbet |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28700 | - |
x * 28700, sequence calculation | Zingiber zerumbet |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-hydroxy-alpha-humulene + NAD+ | Zingiber zerumbet | - |
zerumbone + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zingiber zerumbet | F1SWA0 | gene ZSD1 | - |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from Escherichia coli strain Bl21(DE3) | Zingiber zerumbet |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Zingiber zerumbet | - |
rhizome | contains an exceptionally high level of sesquiterpenoids with zerumbone | Zingiber zerumbet | - |
stem | - |
Zingiber zerumbet | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-hydroxy-alpha-humulene + NAD+ | - |
Zingiber zerumbet | zerumbone + NADH + H+ | - |
? | |
10-hydroxy-alpha-humulene + NAD+ | the enzyme possesses a flexible substrate-binding pocket and converts 10-hydroxy-alpha-humulene into zerumbone | Zingiber zerumbet | zerumbone + NADH + H+ | - |
? | |
additional information | the enzyme also converts borneol to camphor in vitro with Km and kcat values of 0.0228 mM and 4.1/s | Zingiber zerumbet | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 28700, sequence calculation | Zingiber zerumbet |
More | a 3D structure model of ZSD1 reveals a large, flexible substrate-binding pocket, molecular modeling, overview | Zingiber zerumbet |
Synonyms | Comment | Organism |
---|---|---|
ZSD1 | - |
Zingiber zerumbet |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Zingiber zerumbet |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
NAD+ | recombinant mutant S142A, pH 8.0, 37°C | Zingiber zerumbet | |
0.1 | - |
10-hydroxy-alpha-humulene | recombinant mutant S142A, pH 8.0, 37°C | Zingiber zerumbet | |
0.1 | - |
NAD+ | recombinant mutant Y155A, pH 8.0, 37°C | Zingiber zerumbet | |
0.1 | - |
10-hydroxy-alpha-humulene | recombinant mutant Y155A, pH 8.0, 37°C | Zingiber zerumbet | |
1.3 | - |
10-hydroxy-alpha-humulene | recombinant wild-type enzyme, pH 8.0, 37°C | Zingiber zerumbet | |
2.9 | - |
10-hydroxy-alpha-humulene | recombinant mutant S144A, pH 8.0, 37°C | Zingiber zerumbet | |
3.8 | - |
NAD+ | recombinant wild-type enzyme, pH 8.0, 37°C | Zingiber zerumbet | |
4.4 | - |
NAD+ | recombinant mutant S144A, pH 8.0, 37°C | Zingiber zerumbet |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Zingiber zerumbet |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | ZSD1 contains an NAD+-binding motif TGxxxGxG | Zingiber zerumbet |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Zingiber zerumbet | sequence calculation | - |
5.63 |
General Information | Comment | Organism |
---|---|---|
evolution | ZSD1 is a member of the short-chain dehydrogenase/reductase superfamily, SDR, and shares high identities with other plant SDRs involved in secondary metabolism, stress responses and phytosteroid biosynthesis | Zingiber zerumbet |
metabolism | ZSD1 is an alcohol dehydrogenase capable of catalyzing the final step of zerumbone biosynthesis, but ZSD1 is also be involved in other biological processes | Zingiber zerumbet |
additional information | the enzyme contains an active site sequence 155Y-X-X-X-K159, Ser142 completes the Ser-Tyr-Lys triad responsible for the catalysis of ZSD1 | Zingiber zerumbet |
physiological function | ZSD1 is involved in secondary metabolism, stress responses and phytosteroid biosynthesis | Zingiber zerumbet |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000007 | - |
10-hydroxy-alpha-humulene | recombinant mutant S142A, pH 8.0, 37°C | Zingiber zerumbet | |
0.0000008 | - |
NAD+ | recombinant mutant S142A, pH 8.0, 37°C | Zingiber zerumbet | |
0.000001 | - |
10-hydroxy-alpha-humulene | recombinant mutant Y155A, pH 8.0, 37°C | Zingiber zerumbet | |
0.000002 | - |
NAD+ | recombinant mutant Y155A, pH 8.0, 37°C | Zingiber zerumbet | |
0.000022 | - |
10-hydroxy-alpha-humulene | recombinant wild-type enzyme, pH 8.0, 37°C | Zingiber zerumbet | |
0.000103 | - |
10-hydroxy-alpha-humulene | recombinant mutant S144A, pH 8.0, 37°C | Zingiber zerumbet | |
0.000139 | - |
NAD+ | recombinant wild-type enzyme, pH 8.0, 37°C | Zingiber zerumbet | |
0.000262 | - |
NAD+ | recombinant mutant S144A, pH 8.0, 37°C | Zingiber zerumbet |