Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Paenarthrobacter nicotinovorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0062 | - |
1,4-Naphthoquinone | pH 7.1, temperature not specified in the publication | Paenarthrobacter nicotinovorans | |
0.0088 | - |
1,4-benzoquinone | pH 7.1, temperature not specified in the publication | Paenarthrobacter nicotinovorans | |
0.023 | - |
oxidized 2,6-dichlorophenol-indophenol | pH 7.1, temperature not specified in the publication | Paenarthrobacter nicotinovorans | |
0.035 | - |
NADH | pH 7.1, temperature not specified in the publication | Paenarthrobacter nicotinovorans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenarthrobacter nicotinovorans | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.004 | - |
substrate duraquinone, pH 7.1, temperature not specified in the publication | Paenarthrobacter nicotinovorans |
0.102 | - |
substrate menadione, pH 7.1, temperature not specified in the publication | Paenarthrobacter nicotinovorans |
0.368 | - |
substrate 2,6-dichlorophenol-indophenol, pH 7.1, temperature not specified in the publication | Paenarthrobacter nicotinovorans |
0.536 | - |
substrate 1,4-benzoquinone, pH 7.1, temperature not specified in the publication | Paenarthrobacter nicotinovorans |
0.733 | - |
substrate 1,4-naphthoquinone, pH 7.1, temperature not specified in the publication | Paenarthrobacter nicotinovorans |
0.893 | - |
substrate nicotine blue, pH 7.1, temperature not specified in the publication | Paenarthrobacter nicotinovorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,4-benzoquinone + NADPH + H+ | - |
Paenarthrobacter nicotinovorans | 1,4-benzoquinol + NADP+ | - |
? | |
1,4-naphthoquinone + NADPH + H+ | - |
Paenarthrobacter nicotinovorans | 1,4-naphthoquinol + NADP+ | - |
? | |
4,4',5,5'-tetrahydroxy-3,3'-diazadiphenoquinone-(2,2') + NADPH + H+ | - |
Paenarthrobacter nicotinovorans | ? + NADP+ | - |
? | |
duroquinone + NADPH + H+ | - |
Paenarthrobacter nicotinovorans | duroquinol + NADP+ | - |
? | |
menadione + NADPH + H+ | - |
Paenarthrobacter nicotinovorans | menadiol + NADP+ | - |
? | |
nicotine blue + NADH + H+ | - |
Paenarthrobacter nicotinovorans | nicotine blue leuco form + NAD+ | two-electron transfer reaction | ? | |
nicotine blue + NADPH + H+ | - |
Paenarthrobacter nicotinovorans | nicotine blue leuco form + NADP+ | two-electron transfer reaction | ? | |
oxidized 2,6-dichlorophenol-indophenol + NADH + H+ | - |
Paenarthrobacter nicotinovorans | reduced 2,6-dichlorophenol-indophenol + NAD+ | - |
r | |
oxidized 2,6-dichlorophenol-indophenol + NADPH + H+ | - |
Paenarthrobacter nicotinovorans | reduced 2,6-dichlorophenol-indophenol + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NAD(P)H-nicotine blue oxidoreductase | - |
Paenarthrobacter nicotinovorans |
NAD(P)H-nicotine blue quinone oxidoreductase | - |
Paenarthrobacter nicotinovorans |
nboR | - |
Paenarthrobacter nicotinovorans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Paenarthrobacter nicotinovorans | |
NADH | NADH is prefered over NADPH | Paenarthrobacter nicotinovorans | |
NADPH | 70% of the rate with NADH | Paenarthrobacter nicotinovorans |
Organism | Comment | Expression |
---|---|---|
Paenarthrobacter nicotinovorans | enzyme gene is expressed in a nicotine-dependent manner under the control of the transcriptional activator PmfR | additional information |
General Information | Comment | Organism |
---|---|---|
physiological function | enzyme is part of the nicotine catabolic pathway | Paenarthrobacter nicotinovorans |