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Literature summary for 1.1.1.328 extracted from

  • Mihasan, M.; Chiribau, C.B.; Friedrich, T.; Artenie, V.; Brandsch, R.
    An NAD(P)H-nicotine blue oxidoreductase is part of the nicotine regulon and may protect Arthrobacter nicotinovorans from oxidative stress during nicotine catabolism (2007), Appl. Environ. Microbiol., 73, 2479-2485.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Paenarthrobacter nicotinovorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0062
-
1,4-Naphthoquinone pH 7.1, temperature not specified in the publication Paenarthrobacter nicotinovorans
0.0088
-
1,4-benzoquinone pH 7.1, temperature not specified in the publication Paenarthrobacter nicotinovorans
0.023
-
oxidized 2,6-dichlorophenol-indophenol pH 7.1, temperature not specified in the publication Paenarthrobacter nicotinovorans
0.035
-
NADH pH 7.1, temperature not specified in the publication Paenarthrobacter nicotinovorans

Organism

Organism UniProt Comment Textmining
Paenarthrobacter nicotinovorans
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.004
-
substrate duraquinone, pH 7.1, temperature not specified in the publication Paenarthrobacter nicotinovorans
0.102
-
substrate menadione, pH 7.1, temperature not specified in the publication Paenarthrobacter nicotinovorans
0.368
-
substrate 2,6-dichlorophenol-indophenol, pH 7.1, temperature not specified in the publication Paenarthrobacter nicotinovorans
0.536
-
substrate 1,4-benzoquinone, pH 7.1, temperature not specified in the publication Paenarthrobacter nicotinovorans
0.733
-
substrate 1,4-naphthoquinone, pH 7.1, temperature not specified in the publication Paenarthrobacter nicotinovorans
0.893
-
substrate nicotine blue, pH 7.1, temperature not specified in the publication Paenarthrobacter nicotinovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,4-benzoquinone + NADPH + H+
-
Paenarthrobacter nicotinovorans 1,4-benzoquinol + NADP+
-
?
1,4-naphthoquinone + NADPH + H+
-
Paenarthrobacter nicotinovorans 1,4-naphthoquinol + NADP+
-
?
4,4',5,5'-tetrahydroxy-3,3'-diazadiphenoquinone-(2,2') + NADPH + H+
-
Paenarthrobacter nicotinovorans ? + NADP+
-
?
duroquinone + NADPH + H+
-
Paenarthrobacter nicotinovorans duroquinol + NADP+
-
?
menadione + NADPH + H+
-
Paenarthrobacter nicotinovorans menadiol + NADP+
-
?
nicotine blue + NADH + H+
-
Paenarthrobacter nicotinovorans nicotine blue leuco form + NAD+ two-electron transfer reaction ?
nicotine blue + NADPH + H+
-
Paenarthrobacter nicotinovorans nicotine blue leuco form + NADP+ two-electron transfer reaction ?
oxidized 2,6-dichlorophenol-indophenol + NADH + H+
-
Paenarthrobacter nicotinovorans reduced 2,6-dichlorophenol-indophenol + NAD+
-
r
oxidized 2,6-dichlorophenol-indophenol + NADPH + H+
-
Paenarthrobacter nicotinovorans reduced 2,6-dichlorophenol-indophenol + NADP+
-
?

Synonyms

Synonyms Comment Organism
NAD(P)H-nicotine blue oxidoreductase
-
Paenarthrobacter nicotinovorans
NAD(P)H-nicotine blue quinone oxidoreductase
-
Paenarthrobacter nicotinovorans
nboR
-
Paenarthrobacter nicotinovorans

Cofactor

Cofactor Comment Organism Structure
FMN
-
Paenarthrobacter nicotinovorans
NADH NADH is prefered over NADPH Paenarthrobacter nicotinovorans
NADPH 70% of the rate with NADH Paenarthrobacter nicotinovorans

Expression

Organism Comment Expression
Paenarthrobacter nicotinovorans enzyme gene is expressed in a nicotine-dependent manner under the control of the transcriptional activator PmfR additional information

General Information

General Information Comment Organism
physiological function enzyme is part of the nicotine catabolic pathway Paenarthrobacter nicotinovorans