Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.33 extracted from

  • Beedle, A.S.; Rees, H.H.; Goodwin, T.W.
    Some properties and a suggested reclassification of mevaldate reductase (1974), Biochem. J., 139, 205-209.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.33

Inhibitors

Inhibitors Comment Organism Structure
Sodium aminobarbitone 1 mM, 48% inhibition, noncompetitive Sus scrofa
Thiobarbituric acid 1 mM, 22% inhibition, noncompetitive Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.09
-
 NADPH
-
 Sus scrofa
0.15
-
 4-cyanobenzaldehyde
-
 Sus scrofa
0.26
-
 (3RS)-mevaldate
-
 Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 gel filtration Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-3-hydroxy-3-methyl-5-oxovaleric acid + NADPH Sus scrofa
-
 (R)-3-hydroxy-3-methyl-5-hydroxyvaleric acid + NADP+
-
 ir

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, calcium phosphate gel, gel filtration Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.573
-
-
 Sus scrofa

Storage Stability

Storage Stability Organism
-20°C, 3 months, 0% loss of activity Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(3RS)-mevaldate + NADPH + H+
-
 Sus scrofa (R)-mevalonate + NADP+
-
 r
(R)-3-hydroxy-3-methyl-5-oxovaleric acid + NADPH
-
 Sus scrofa (R)-3-hydroxy-3-methyl-5-hydroxyvaleric acid + NADP+
-
 ir
(S)-3-hydroxy-3-methyl-5-oxovaleric acid + NADH
-
 Sus scrofa (S)-3-hydroxy-3-methyl-5-hydroxyvaleric acid + NAD+
-
 ir
4-cyanobenzaldehyde + NADPH
-
 Sus scrofa 4-cyanobenzylalcohol + NADP+
-
 ir
pyridine-3-carboxaldehyde + NADPH
-
 Sus scrofa pyridine-3-methanol + NADP+
-
 ir

Cofactor

Cofactor Comment Organism Structure
NADH giving one-fifth of the rate obtained with NADPH Sus scrofa
NADPH
-
 Sus scrofa