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Literature summary for 1.1.1.335 extracted from

  • Westman, E.L.; McNally, D.J.; Charchoglyan, A.; Brewer, D.; Field, R.A.; Lam, J.S.
    Characterization of WbpB, WbpE, and WbpD and reconstitution of a pathway for the biosynthesis of UDP-2,3-diacetamido-2,3-dideoxy-D-mannuronic acid in Pseudomonas aeruginosa (2009), J. Biol. Chem., 284, 11854-11862.
    View publication on PubMedView publication on EuropePMC
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Application

Application Comment Organism
synthesis synthesis of preparative quantities of 2-acetamido-3-amino-2,3-dideoxy-D-glucuronic acid and 2,3-diacetamido-2,3-dideoxy-D-glucuronic acid by coupled reaction of enzyme WbpB and transaminase WpbE Pseudomonas aeruginosa

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa G3XD23
-
-

Storage Stability

Storage Stability Organism
-20°C, stable for at least 3 weeks Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-acetamido-2-deoxy-alpha-D-glucuronic acid + NAD+ + L-glutamate activity of enzyme His6-WbpB is detected only in presence of transaminase WbpE, and vice versa Pseudomonas aeruginosa UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronic acid + NADH + 2-ketoglutarate product of the coupled reaction of enzyme WbpB and transaminase WbpE ?