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Literature summary for 1.1.1.335 extracted from

  • Thoden, J.; Holden, H.
    Biochemical and structural characterization of WlbA from Bordetella pertussis and Chromobacterium violaceum: Enzymes required for the biosynthesis of 2,3-diacetamido-2,3-dideoxy-D-mannuronic acid (2011), Biochemistry, 50, 1483-1491.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bordetella pertussis
expression in Escherichia coli Chromobacterium violaceum

Crystallization (Commentary)

Crystallization (Comment) Organism
in presence of NAD(H) and substrate to 2.13 A and 1.5 A resolution. Enzyme displays octameric quaternary structure with the active sites positioned far apart. The octamers can be envisioned as tetramers of dimers. The carboxylate group attached to the C-5' carbon of the hexose in the natural substrate, UDP-N-acetyl-D-glucosaminuronic acid, is held firmly in place in the enzyme WlbA active site by the side chains of Arg165 and Tyr169 Bordetella pertussis
in presence of NAD(H) and substrate to 2.13 A and 1.5 A resolution. Enzyme displays octameric quaternary structure with the active sites positioned far apart. The octamers can be envisioned as tetramers of dimers. The carboxylate group attached to the C-5' carbon of the hexose in the natural substrate, UDP-N-acetyl-D-glucosaminuronic acid, is held firmly in place in the enzyme WlbA active site by the side chains of Arg165 and Tyr169 Chromobacterium violaceum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0056
-
UDP-2-acetamido-2-deoxy-alpha-D-glucuronate presence of transaminase WlbC, 25°C, pH 8.5 Chromobacterium violaceum
0.015
-
UDP-2-acetamido-2-deoxy-alpha-D-glucuronate presence of transaminase WlbC, 25°C, pH 8.5 Bordetella pertussis
0.2
-
UDP-alpha-D-glucuronate 25°C, pH 8.5 Chromobacterium violaceum
0.47
-
NAD+ 25°C, pH 8.5 Chromobacterium violaceum
0.97
-
NAD+ 25°C, pH 8.5 Bordetella pertussis
1.1
-
UDP-2-acetamido-2-deoxy-alpha-D-glucuronate 25°C, pH 8.5 Chromobacterium violaceum
2.05
-
UDP-alpha-D-glucuronate 25°C, pH 8.5 Bordetella pertussis
2.36
-
UDP-2-acetamido-2-deoxy-alpha-D-glucuronate 25°C, pH 8.5 Bordetella pertussis

Organism

Organism UniProt Comment Textmining
Bordetella pertussis
-
-
-
Chromobacterium violaceum Q7NQL0 putative
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the reaction mechanism is sequential, enzyme does not require 2-ketoglutarate for activity. No substrates: UDP-glucose, UDP-galactose, UDP-N-acetylglucosamine Bordetella pertussis ?
-
?
additional information the reaction mechanism is sequential, enzyme does not require 2-oxoglutarate for activity. No substrates: UDP-glucose, UDP-galactose, UDP-N-acetylglucosamine Chromobacterium violaceum ?
-
?
UDP-2-acetamido-2-deoxy-alpha-D-glucuronate + NAD+
-
Bordetella pertussis UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate + NADH + H+
-
?
UDP-2-acetamido-2-deoxy-alpha-D-glucuronate + NAD+
-
Chromobacterium violaceum UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate + NADH + H+
-
?
UDP-alpha-D-glucuronate + NAD+
-
Bordetella pertussis UDP-alpha-D-ribo-hex-3-uluronate + NADH + H+
-
?
UDP-alpha-D-glucuronate + NAD+
-
Chromobacterium violaceum UDP-alpha-D-ribo-hex-3-uluronate + NADH + H+
-
?

Synonyms

Synonyms Comment Organism
WlbA
-
Bordetella pertussis
WlbA
-
Chromobacterium violaceum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00015
-
UDP-alpha-D-glucuronate 25°C, pH 8.5 Chromobacterium violaceum
0.00031
-
UDP-2-acetamido-2-deoxy-alpha-D-glucuronate 25°C, pH 8.5 Chromobacterium violaceum
0.00076
-
UDP-2-acetamido-2-deoxy-alpha-D-glucuronate presence of transaminase WlbC, 25°C, pH 8.5 Chromobacterium violaceum
0.0021
-
UDP-alpha-D-glucuronate 25°C, pH 8.5 Bordetella pertussis
0.0039
-
UDP-2-acetamido-2-deoxy-alpha-D-glucuronate presence of transaminase WlbC, 25°C, pH 8.5 Bordetella pertussis
0.0047
-
UDP-2-acetamido-2-deoxy-alpha-D-glucuronate 25°C, pH 8.5 Bordetella pertussis

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Bordetella pertussis
NAD+
-
Chromobacterium violaceum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.00028
-
UDP-2-acetamido-2-deoxy-alpha-D-glucuronate 25°C, pH 8.5 Chromobacterium violaceum
0.00075
-
UDP-alpha-D-glucuronate 25°C, pH 8.5 Chromobacterium violaceum
0.001
-
UDP-alpha-D-glucuronate 25°C, pH 8.5 Bordetella pertussis
0.002
-
UDP-2-acetamido-2-deoxy-alpha-D-glucuronate 25°C, pH 8.5 Bordetella pertussis
0.136
-
UDP-2-acetamido-2-deoxy-alpha-D-glucuronate presence of transaminase WlbC, 25°C, pH 8.5 Chromobacterium violaceum
0.26
-
UDP-2-acetamido-2-deoxy-alpha-D-glucuronate presence of transaminase WlbC, 25°C, pH 8.5 Bordetella pertussis