Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli strain BL21(DE3) as soluble protein | Trypanosoma cruzi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cerivastatin | biphasic inhibition mechanism, slow, tight-binding type of inhibitor | Trypanosoma cruzi | |
fluvastatin | competitive | Trypanosoma cruzi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic analysis | Trypanosoma cruzi | |
0.013 | - |
(S)-3-hydroxy-3-methylglutaryl-CoA | pH 6.8, 25°C | Trypanosoma cruzi | |
0.043 | - |
NADPH | pH 6.8, 25°C | Trypanosoma cruzi | |
0.074 | - |
CoA | pH 8.3, 25°C | Trypanosoma cruzi | |
0.22 | - |
NADP+ | pH 8.3, 25°C | Trypanosoma cruzi | |
0.252 | - |
(R)-mevalonate | pH 8.3, 25°C | Trypanosoma cruzi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+ | Trypanosoma cruzi | - |
(R)-mevalonate + CoA + 2 NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | - |
- |
- |
Purification (Comment) | Organism |
---|---|
3.8fold, recombinant enzyme from Escherichia coli | Trypanosoma cruzi |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
28 | - |
purified recombinant enzyme | Trypanosoma cruzi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+ | - |
Trypanosoma cruzi | (R)-mevalonate + CoA + 2 NADP+ | - |
r | |
(S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+ | the reduction of 3-hydroxy-3-methylglutaryl-CoA is preferred over oxidation of mevalonate | Trypanosoma cruzi | (R)-mevalonate + CoA + 2 NADP+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Trypanosoma cruzi |
Synonyms | Comment | Organism |
---|---|---|
3-hydroxy-3-methylglutaryl CoA reductase | - |
Trypanosoma cruzi |
HMGR | - |
Trypanosoma cruzi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Trypanosoma cruzi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.7 | 6.3 | reduction of (S)-3-hydroxy-3-methylglutaryl-CoA | Trypanosoma cruzi |
8.7 | 9.6 | oxidation of (R)-mevalonate | Trypanosoma cruzi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Trypanosoma cruzi |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.027 | - |
fluvastatin | pH 6.8, 25°C, versus (S)-3-hydroxy-3-methylglutaryl-CoA | Trypanosoma cruzi |