Crystallization (Comment) | Organism |
---|---|
enzyme in complex with inhibitor lovastatin, from 1.2 M ammonium sulfate, at 20°C, a few days, larger crystals by microseeding, complexing with the inhibitor by soaking in a KOH solution, pH 9.0, containing 2 mM lovastatin, X-ray diffraction structure determination and analysis at 2.6 A resolution | Pseudomonas mevalonii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
lovastatin | slightly inhibitory statin for class II enzyme, binding structure at the active site involves the residues Lys267, Asn271, Glu83, Arg261, and 2 water molecules, substrate mimicking binding mode | Pseudomonas mevalonii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas mevalonii | - |
class II enzyme | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(R)-mevalonate + CoA + 2 NADP+ = (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+ | via mevaldehyde as an intermediate product, enzyme contains a catalytic His381, Lys267 is also involved in catalysis, active site structure | Pseudomonas mevalonii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+ | overall reaction | Pseudomonas mevalonii | (R)-mevalonate + CoA + 2 NADP+ | - |
r | |
(S)-3-hydroxy-3-methylglutaryl-CoA + NADPH + H+ | first step reaction | Pseudomonas mevalonii | mevaldyl-CoA + NADP+ | - |
r | |
mevaldehyde + NADPH + H+ | third step reaction | Pseudomonas mevalonii | (R)-mevalonate + NADP+ | - |
r | |
mevaldyl-CoA + H2O | second step reaction | Pseudomonas mevalonii | mevaldehyde + NADP+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
3-hydroxy-3-methylglutaryl CoA reductase | - |
Pseudomonas mevalonii |