Literature summary for 1.1.1.345 extracted from

Bernard, N.; Johnsen, K.; Ferain, T.; Garmyn, D.; Hols, P.; Holbrook, J.J.; Delcour, J.
NAD(+)-dependent D-2-hydroxyisocaproate dehydrogenase of Lactobacillus delbrueckii subsp. bulgaricus. Gene cloning and enzyme characterization (1994), Eur. J. Biochem., 224, 439-446.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Lactobacillus delbrueckii

Inhibitors

Inhibitors	Comment	Organism	Structure
2-Oxohexanoate	substrate inhibition	Lactobacillus delbrueckii
2-oxopentanoate	substrate inhibition	Lactobacillus delbrueckii
4-methyl-2-oxopentanoate	substrate inhibition	Lactobacillus delbrueckii
phenylpyruvate	substrate inhibition	Lactobacillus delbrueckii

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.02	-	2-Oxohexanoate	pH 7.5, 25°C	Lactobacillus delbrueckii
0.022	-	4-methyl-2-oxopentanoate	pH 7.5, 25°C	Lactobacillus delbrueckii
0.031	-	phenylpyruvate	pH 7.5, 25°C	Lactobacillus delbrueckii
0.057	-	2-oxopentanoate	pH 7.5, 25°C	Lactobacillus delbrueckii

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
36948	-	x * 36948, calculated from sequence	Lactobacillus delbrueckii

Organism

Organism	UniProt	Comment	Textmining
Lactobacillus delbrueckii	Q48534	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Lactobacillus delbrueckii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-oxohexanoate + NADH + H+	i.e. 2-oxocaproate	Lactobacillus delbrueckii	2-hydroxyhexanoate + NAD+	-	?
2-oxopentanoate + NADH + H+	i.e. 2-oxovalerate	Lactobacillus delbrueckii	2-hydroxypentanoate + NAD+	-	?
4-methyl-2-oxopentanoate + NADH + H+	i.e. 2-oxoisocaproate	Lactobacillus delbrueckii	(R)-2-hydroxy-4-methylpentanoate + NAD+	i.e. (R)-2-hydroxyisocaproate	?
phenylpyruvate + NADH + H+	-	Lactobacillus delbrueckii	phenyllactate + NAD+	-	?

Subunits

Subunits	Comment	Organism
?	x * 36948, calculated from sequence	Lactobacillus delbrueckii

Synonyms

Synonyms	Comment	Organism
D-2-hydroxyisocaproate dehydrogenase	-	Lactobacillus delbrueckii
HdhD	-	Lactobacillus delbrueckii

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Lactobacillus delbrueckii

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Lactobacillus delbrueckii

Cofactor

Cofactor	Comment	Organism	Structure
NADH	-	Lactobacillus delbrueckii

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Release 2023.1 (January 2023)