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Literature summary for 1.1.1.346 extracted from

  • Miller, J.V.; Estell, D.A.; Lazarus, R.A.
    Purification and characterization of 2,5-diketo-D-gluconate reductase from Corynebacterium sp (1987), J. Biol. Chem., 262, 9016-9020.
    View publication on PubMed
Search for more literature for 1.1.1.346

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ strong inhibition at 0.5 mM Corynebacterium sp.
Fe3+ strong inhibition at 0.5 mM Corynebacterium sp.
additional information not inhibited by 2-dehydro-L-gulonate. There is no observed effect on activity by 0.5 mM solutions of Mg2+, Mn2+, Ca2+, or Co2+, 14 mM 2-mercaptoethanol, 1 mM dithiothreitol, or 1 mM EDTA Corynebacterium sp.
NADP+ competitive inhibition Corynebacterium sp.
Ni2+ strong inhibition at 0.5 mM Corynebacterium sp.
Zn2+ strong inhibition at 0.5 mM Corynebacterium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 NADPH at pH 6.4 and 25°C Corynebacterium sp.
0.125
-
 NADP+ at pH 9.2 and 25°C Corynebacterium sp.
26
-
 2,5-didehydro-D-gluconate at pH 6.4 and 25°C Corynebacterium sp.
155
-
 5-dehydro-D-fructose at pH 6.4 and 25°C Corynebacterium sp.
160
-
 dihydroxyacetone at pH 6.4 and 25°C Corynebacterium sp.
204
-
 2-dehydro-L-gulonate at pH 9.2 and 25°C Corynebacterium sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Corynebacterium sp. 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
 1 * 34000, SDS-PAGE Corynebacterium sp.
35000
-
 gel filtration Corynebacterium sp.

Organism

Organism UniProt Comment Textmining
Corynebacterium sp.
-
-
-
Corynebacterium sp. ATCC 31090
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-cellulose column chromatography, Cibacron blueF 3GA affinity column chromatography, and and TSK gel filtration Corynebacterium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0052
-
 crude extract, at pH 6.4 and 25°C Corynebacterium sp.
3.38
-
 after 650fold purification, at pH 6.4 and 25°C Corynebacterium sp.

Storage Stability

Storage Stability Organism
-70°C, 2 mg/ml enzyme in 20 mM Tris-HC1 (pH 7.5), 6 months, the enzyme remains stable Corynebacterium sp.
4°C, enzyme solution at pH 6.5-7.5, at least 2 months, the enzyme remains stable Corynebacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,5-didehydro-D-gluconate + NADPH + H+ a reduction of substrate by NADPH is highly preferred Corynebacterium sp. 2-dehydro-L-gulonate + NADP+
-
 r
2,5-didehydro-D-gluconate + NADPH + H+ a reduction of substrate by NADPH is highly preferred Corynebacterium sp. ATCC 31090 2-dehydro-L-gulonate + NADP+
-
 r
2-dehydro-L-gulonate + NADP+
-
 Corynebacterium sp. 2,5-didehydro-D-gluconate + NADPH + H+
-
 r
5-dehydro-D-fructose + NADPH + H+
-
 Corynebacterium sp. ? + NADP+
-
 ?
dihydroxyacetone + NADPH + H+
-
 Corynebacterium sp. ? + NADP+
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 34000, SDS-PAGE Corynebacterium sp.

Synonyms

Synonyms Comment Organism
2,5-diketo-D-gluconate reductase
-
 Corynebacterium sp.
2,5-DKG reductase
-
 Corynebacterium sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
8.3
-
 2,5-didehydro-D-gluconate at pH 6.4 and 25°C Corynebacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4
-
 for the reduction of 2,5-didehydro-D-gluconate Corynebacterium sp.
9.2
-
 for the oxidation of 2-dehydro-L-gulonate Corynebacterium sp.

pH Range

pH Minimum pH Maximum Comment Organism
5 8 for the reduction of 2,5-didehydro-D-gluconate Corynebacterium sp.

Cofactor

Cofactor Comment Organism Structure
NADPH dependent on Corynebacterium sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.026
-
 NADP+ at pH 9.2 and 25°C Corynebacterium sp.

pI Value

Organism Comment pI Value Maximum pI Value
Corynebacterium sp. isoelectric focusing
-
 4.4