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Literature summary for 1.1.1.35 extracted from

  • Bradshaw, R.A.; Noyes, B.E.
    L-3-hydroxylacyl coenzyme A dehydrogenase from pig heart muscle (1975), Methods Enzymol., 35, 122-128.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
50% saturation with ammonium sulfate solution, 0.1 M potassium phosphate, pH 6.8, 1 mM EDTA, 2 mM beta-mercaptoethanol, 4°C, crystals appear after 2 days Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoic acid)
-
Sus scrofa
iodoacetamide
-
Sus scrofa
iodoacetic acid
-
Sus scrofa
N-bromsuccinimide
-
Sus scrofa
N-ethylmaleimide
-
Sus scrofa
p-chloromercuribenzoate
-
Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
acetoacetyl-CoA
-
Sus scrofa
0.4
-
acetoacetyl-pantetheine
-
Sus scrofa
10
-
acetoacetyl-Nacetylcysteamine
-
Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Sus scrofa 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
sedimentation equilibrium Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-3-hydroxybutyryl-CoA + NAD+ Sus scrofa
-
acetoacetyl-CoA + NADH
-
r

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, first CM-cellulose, gelfiltration, second and third CM-cellulose Sus scrofa

Renatured (Commentary)

Renatured (Comment) Organism
after denaturation with 2-3 M urea or guanidinium-HCl, 94% activity can be recovered by dialyzing the enzyme at 4°C against 100 mM sodium diphosphate, pH 7.3, 10 mM 2-mercaptoethanol Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
heart
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
220
-
-
Sus scrofa

Storage Stability

Storage Stability Organism
4°C, 90% ammonium sulfate, at least 6 monts, no loss of activity Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-3-hydroxybutyryl-CoA + NAD+
-
Sus scrofa acetoacetyl-CoA + NADH
-
r
acetoacetyl-N-acetylcysteamine + NADH
-
Sus scrofa (S)-3-hydroxybutyryl-N-acetylcysteamine + NAD+
-
?
acetoacetyl-pantetheine + NADH
-
Sus scrofa (S)-3-hydroxybutyryl-pantetheine
-
?

Subunits

Subunits Comment Organism
dimer
-
Sus scrofa

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Sus scrofa