Crystallization (Comment) | Organism |
---|---|
50% saturation with ammonium sulfate solution, 0.1 M potassium phosphate, pH 6.8, 1 mM EDTA, 2 mM beta-mercaptoethanol, 4°C, crystals appear after 2 days | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) | - |
Sus scrofa | |
iodoacetamide | - |
Sus scrofa | |
iodoacetic acid | - |
Sus scrofa | |
N-bromsuccinimide | - |
Sus scrofa | |
N-ethylmaleimide | - |
Sus scrofa | |
p-chloromercuribenzoate | - |
Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
acetoacetyl-CoA | - |
Sus scrofa | |
0.4 | - |
acetoacetyl-pantetheine | - |
Sus scrofa | |
10 | - |
acetoacetyl-Nacetylcysteamine | - |
Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Sus scrofa | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
sedimentation equilibrium | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-3-hydroxybutyryl-CoA + NAD+ | Sus scrofa | - |
acetoacetyl-CoA + NADH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, first CM-cellulose, gelfiltration, second and third CM-cellulose | Sus scrofa |
Renatured (Comment) | Organism |
---|---|
after denaturation with 2-3 M urea or guanidinium-HCl, 94% activity can be recovered by dialyzing the enzyme at 4°C against 100 mM sodium diphosphate, pH 7.3, 10 mM 2-mercaptoethanol | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
220 | - |
- |
Sus scrofa |
Storage Stability | Organism |
---|---|
4°C, 90% ammonium sulfate, at least 6 monts, no loss of activity | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-3-hydroxybutyryl-CoA + NAD+ | - |
Sus scrofa | acetoacetyl-CoA + NADH | - |
r | |
acetoacetyl-N-acetylcysteamine + NADH | - |
Sus scrofa | (S)-3-hydroxybutyryl-N-acetylcysteamine + NAD+ | - |
? | |
acetoacetyl-pantetheine + NADH | - |
Sus scrofa | (S)-3-hydroxybutyryl-pantetheine | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Sus scrofa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Sus scrofa |