Cloned (Comment) | Organism |
---|---|
expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
N208A | site-directed mutagenesis, the substrate binding of the mutant enzyme is affected | Rattus norvegicus |
S137A | site-directed mutagenesis, the substrate binding of the mutant enzyme is affected | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of wild-type and mutant enzymes, overview | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-3-hydroxyacyl-CoA + NAD+ | Rattus norvegicus | - |
3-oxoacyl-CoA + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Rattus norvegicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH + H+ | reaction mechanism via enolate intermediate | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-3-hydroxyacyl-CoA + NAD+ | - |
Rattus norvegicus | 3-oxoacyl-CoA + NADH + H+ | - |
r | |
(S)-3-hydroxyacyl-CoA + NAD+ | the enzyme is a B-side-specific dehydrogenase with hydride transfer occurring on the si face of the nicotinamide ring | Rattus norvegicus | 3-oxoacyl-CoA + NADH + H+ | - |
r | |
2-fluoro-3-hydroxy-4-octenoyl-CoA + NAD+ | - |
Rattus norvegicus | 2-fluoro-3-oxo-4-octenoyl-CoA + NADH | - |
r | |
2-fluoro-3-hydroxyoctanoyl-CoA + NAD+ | - |
Rattus norvegicus | 2-fluoro-3-oxooctanoyl-CoA + NADH | - |
r | |
3-hydroxy-4-octenoyl-CoA + NAD+ | - |
Rattus norvegicus | 3-oxo-4-octenoyl-CoA + NADH | - |
r | |
3-hydroxyoctanoyl-CoA + NAD+ | - |
Rattus norvegicus | 3-oxooctanoyl-CoA + NADH | - |
r | |
additional information | no activity of the wild-type enzyme with 2,2-difluoro-3-hydroxyoctanoyl-CoA and 2,2-difluoro-3-hydroxy-4-octenoyl-CoA, substrate specificity of wild-type and mutant enzymes, y, overview | Rattus norvegicus | ? | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Rattus norvegicus |