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Literature summary for 1.1.1.351 extracted from

  • Wang, Y.; Zhang, Y.H.
    Overexpression and simple purification of the Thermotoga maritima 6-phosphogluconate dehydrogenase in Escherichia coli and its application for NADPH regeneration (2009), Microb. Cell Fact., 8, 30.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.351

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Thermotoga maritima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 NAD+ in 50 mM HEPES buffer (pH 7.5), at 80°C Thermotoga maritima
0.011
-
 6-phospho-D-gluconate with NADP+ as cosubstrate, in 50 mM HEPES buffer (pH 7.5), at 80°C Thermotoga maritima
0.38
-
 NADP+ in 50 mM HEPES buffer (pH 7.5), at 80°C Thermotoga maritima

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
 x * 53000, SDS-PAGE Thermotoga maritima

Organism

Organism UniProt Comment Textmining
Thermotoga maritima
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-phospho-D-gluconate + NAD+
-
 Thermotoga maritima D-ribulose 5-phosphate + CO2 + NADH + H+
-
 ?
6-phospho-D-gluconate + NADP+
-
 Thermotoga maritima D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 ?

Subunits

Subunits Comment Organism
? x * 53000, SDS-PAGE Thermotoga maritima

Synonyms

Synonyms Comment Organism
6-phosphogluconate dehydrogenase
-
 Thermotoga maritima
6PGDH
-
 Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
-
 Thermotoga maritima

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 60 the enzyme retains about 2 and 20% of its maximum activity at 30 and 60°C, respectively Thermotoga maritima

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60 90 the enzyme has half-life times of 48 and 140 h at 90 and 80°C, respectively. The enzyme retains more than 90% activity for 48 h at 60 and 80°C and retains about 50% enzymatic activity after 48 h at 90°C Thermotoga maritima

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
325
-
 6-phospho-D-gluconate with NADP+ as cosubstrate, in 50 mM HEPES buffer (pH 7.5), at 80°C Thermotoga maritima

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Thermotoga maritima

pH Range

pH Minimum pH Maximum Comment Organism
6 9 about 70% of enzyme activities remain at pH 6.0 and 9.0 Thermotoga maritima

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Thermotoga maritima
NADP+ this enzyme prefers NADP+ to NAD+ as an electron acceptor Thermotoga maritima