Any feedback?
Literature summary for 1.1.1.352 extracted from
- Purification and gene cloning of a dehydrogenase from Lactobacillus brevis that catalyzes a reaction involved in aflatoxin biosynthesis (2008), Biosci. Biotechnol. Biochem., 72, 724-734.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Levilactobacillus brevis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0426 | - |
5'-hydroxyaverantin | in 30 mM potassium phosphate buffer (pH 7.5), at 37°C | Levilactobacillus brevis |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25873 | - |
3 * 25873, calculated from amino acid sequence | Levilactobacillus brevis |
| 25918 | - |
3 * 25918 MALDI TOF mass spectrometry | Levilactobacillus brevis |
| 33000 | - |
3 * 33000, SDS-PAGE | Levilactobacillus brevis |
| 100000 | - |
gel filtration | Levilactobacillus brevis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5'-hydroxyaverantin + NADP+ | Levilactobacillus brevis | - |
5'-oxoaverantin + NADPH + H+ | - |
ir | |
| 5'-hydroxyaverantin + NADP+ | Levilactobacillus brevis IFO 12005 | - |
5'-oxoaverantin + NADPH + H+ | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Levilactobacillus brevis | - |
- |
- |
| Levilactobacillus brevis IFO 12005 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE Sepharose CL-6B column chromatography, phenyl Sepharose column chromatography, Mono Q column chromatography, and Sephacryl S-300 gel filtration | Levilactobacillus brevis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00613 | - |
cell extract, at pH 7.5 and 37°C | Levilactobacillus brevis |
| 0.552 | - |
after 90fold purification, at pH 7.5 and 37°C | Levilactobacillus brevis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5'-hydroxyaverantin + NADP+ | - |
Levilactobacillus brevis | 5'-oxoaverantin + NADPH + H+ | - |
ir | |
| 5'-hydroxyaverantin + NADP+ | - |
Levilactobacillus brevis IFO 12005 | 5'-oxoaverantin + NADPH + H+ | - |
ir | |
| additional information | no activity with NAD+ | Levilactobacillus brevis | ? | - |
? | |
| additional information | no activity with NAD+ | Levilactobacillus brevis IFO 12005 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotrimer | 3 * 33000, SDS-PAGE | Levilactobacillus brevis |
| homotrimer | 3 * 25873, calculated from amino acid sequence | Levilactobacillus brevis |
| homotrimer | 3 * 25918 MALDI TOF mass spectrometry | Levilactobacillus brevis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alcohol dehydrogenase | - |
Levilactobacillus brevis |
| HAVN dehydrogenase | - |
Levilactobacillus brevis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 55 | - |
Levilactobacillus brevis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | no activity is detected above pH 9.0 | Levilactobacillus brevis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | no activity with NAD+ | Levilactobacillus brevis | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
