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Literature summary for 1.1.1.359 extracted from

  • Smith, L.D.; Budgen, N.; Bungard, S.J.; Danson, M.J.; Hough, D.W.
    Purification and characterization of glucose dehydrogenase from the thermoacidophilic archaebacterium Thermoplasma acidophilum (1989), Biochem. J., 261, 973-977.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
acetone 40% (v/v), 40% activity Thermoplasma acidophilum
ethanol 40% (v/v), 30% activity Thermoplasma acidophilum
methanol 40% (v/v), 50% activity Thermoplasma acidophilum
Urea 40% (v/v), 85% activity Thermoplasma acidophilum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
NAD+ Km is superior to 30 mM. The Km value for NAD+ can not be defined precisely as it is impossible to reach saturation of the enzyme Thermoplasma acidophilum
0.113
-
NADP+ pH 7.0, 55°C Thermoplasma acidophilum
10
-
beta-D-glucose pH 7.0, 55°C Thermoplasma acidophilum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
4 * 38000 Thermoplasma acidophilum
155000
-
gel filtration Thermoplasma acidophilum

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone 50% (v/v), no appreciable loss of activity Thermoplasma acidophilum
Ethanol 50% (v/v), no appreciable loss of activity Thermoplasma acidophilum
Methanol 50% (v/v), no appreciable loss of activity Thermoplasma acidophilum
urea 4 M, no appreciable loss of activity Thermoplasma acidophilum

Organism

Organism UniProt Comment Textmining
Thermoplasma acidophilum P13203
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermoplasma acidophilum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
320
-
pH 7.0, 55°C Thermoplasma acidophilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-glucose + NAD+ + H2O
-
Thermoplasma acidophilum D-gluconate + NADH + 2 H+
-
ir
beta-D-glucose + NADP+ + H2O
-
Thermoplasma acidophilum D-gluconate + NADPH + 2 H+
-
ir
D-galactose + NAD+ + H2O
-
Thermoplasma acidophilum D-galactonate + NADH + 2 H+
-
?
D-galactose + NADP+ + H2O the activity is 70% of the activity with beta-D-glucose + NADP+ Thermoplasma acidophilum D-galactonate + NADPH + 2 H+
-
?
additional information no activity with mannose or glucose 6-phosphate Thermoplasma acidophilum ?
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 38000 Thermoplasma acidophilum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
assay at Thermoplasma acidophilum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
catalytic activity is retained after 9 h Thermoplasma acidophilum
75
-
half-life: 3 h Thermoplasma acidophilum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Thermoplasma acidophilum

Cofactor

Cofactor Comment Organism Structure
NAD+ the enzyme is catalytically active with both NADP+ and NAD+. NADP+ and NAD+ bind at the same site on the enzyme Thermoplasma acidophilum
NADP+ the enzyme is catalytically active with both NADP+ and NAD+. NADP+ and NAD+ bind at the same site on the enzyme Thermoplasma acidophilum