Literature summary for 1.1.1.359 extracted from

Bright, J.R.; Byrom, D.; Danson, M.J.; Hough, D.W.; Towner, P.
Cloning, sequencing and expression of the gene encoding glucose dehydrogenase from the thermophilic archaeon Thermoplasma acidophilum (1993), Eur. J. Biochem., 211, 549-554.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichi coli	Thermoplasma acidophilum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.29	-	NADP+	pH 8.0, 55°C	Thermoplasma acidophilum
9.4	-	beta-D-glucose	pH 8.0, 55°C	Thermoplasma acidophilum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
39400	-	4 * 39400, SDS-PAGE	Thermoplasma acidophilum

Organism

Organism	UniProt	Comment	Textmining
Thermoplasma acidophilum	P13203	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Thermoplasma acidophilum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
458	-	pH 8.0, 55°C	Thermoplasma acidophilum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
beta-D-glucose + NADP+ + H2O	-	Thermoplasma acidophilum	D-gluconate + NADPH + 2 H+	-	?

Subunits

Subunits	Comment	Organism
tetramer	4 * 39400, SDS-PAGE	Thermoplasma acidophilum

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
55	-	assay at	Thermoplasma acidophilum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Thermoplasma acidophilum

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Release 2023.1 (January 2023)