Cloned (Comment) | Organism |
---|---|
N-terminal part of amino acids 1-604 is expressed as a C-terminally His-tagged, stable protein in Escherichia coli strain BL21(DE3) | Candida tropicalis |
Crystallization (Comment) | Organism |
---|---|
5 mg/ml purified recombinant, detagged truncated enzyme, amino acid residues 1-604, by vapor diffusion technique, 20°C, in 30% PEG 4000, 0.1 M sodium acetate, pH 4.2, 0.2 M ammonium acetate, equal volume of 0.002 ml of protein and precipitant solution, 11% v/v glycerol as cryoprotectant, X-ray diffraction structure determination and analysis at 2.22-2.36 A resolution, the wild-type enzyme, comprising amino acid residues 1-906, is unstable and not crystallizable | Candida tropicalis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | site-directed mutagenesis, while the wild-type enzyme, comprising amino acid residues 1-906, is unstable and not crystallizable, as is the recombinant truncated version comprising amino acid residues 1-591, the mutants 1-604 and 1-612, with or without further modifications, are stable with different crystallizability, overview | Candida tropicalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida tropicalis | - |
multifunctional enzyme type 2, i.e. MFE-2 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged, truncated enzyme from Escherichia coli, the His-tag is cleaved off by factor Xa | Candida tropicalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the N-terminal enzyme part harbors 2 different (3R)-hydroxyacyl-CoA dehydrogenase activities with different substrate specificities, while the 2-enoyl-CoA hydratase 2 activity is located in the C-terminus | Candida tropicalis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
(3R)-hydroxyacyl-CoA dehydrogenase | - |
Candida tropicalis |
MFE-2 | - |
Candida tropicalis |