Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain SU294 | Leuconostoc mesenteroides |
Protein Variants | Comment | Organism |
---|---|---|
R46A | mutant glucose-6-phosphate dehydrogenases with coenzyme specificity that favors NAD+, whereas the wild-type enzyme prefers NADP+ as coenzyme | Leuconostoc mesenteroides |
R46A | the enzyme's Km and Ki values for NADP+ are greatly increased (2-3 orders of magnitude) | Leuconostoc mesenteroides |
R46C | mutant glucose-6-phosphate dehydrogenases with coenzyme specificity that favors NAD+, whereas the wild-type enzyme prefers NADP+ as coenzyme | Leuconostoc mesenteroides |
R46Q | the enzyme's Km and Ki values for NADP+ are greatly increased (2-3 orders of magnitude) | Leuconostoc mesenteroides |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Leuconostoc mesenteroides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00799 | - |
NADP+ | wild type enzyme, in 33 mM Tris-HCl, pH 7.6, at 25°C | Leuconostoc mesenteroides | |
0.0692 | - |
D-glucose 6-phosphate | wild type enzyme, with NAD+ as cosubstrate, in 33 mM Tris-HCl, pH 7.6, at 25°C | Leuconostoc mesenteroides | |
0.089 | - |
D-glucose 6-phosphate | 25°C, pH 7.6, mutant enzyme R46Q | Leuconostoc mesenteroides | |
0.0891 | - |
D-glucose 6-phosphate | mutant enzyme R46Q, with NAD+ as cosubstrate, in 33 mM Tris-HCl, pH 7.6, at 25°C | Leuconostoc mesenteroides | |
0.114 | - |
D-glucose 6-phosphate | wild type enzyme, with NADP+ as cosubstrate, in 33 mM Tris-HCl, pH 7.6, at 25°C | Leuconostoc mesenteroides | |
0.162 | - |
NAD+ | wild type enzyme, in 33 mM Tris-HCl, pH 7.6, at 25°C | Leuconostoc mesenteroides | |
0.175 | - |
D-glucose 6-phosphate | 25°C, pH 7.6, mutant enzyme R46A | Leuconostoc mesenteroides | |
0.175 | - |
D-glucose 6-phosphate | mutant enzyme R46A, with NAD+ as cosubstrate, in 33 mM Tris-HCl, pH 7.6, at 25°C | Leuconostoc mesenteroides | |
0.233 | - |
NADP+ | 25°C, pH 7.6, mutant enzyme R46Q | Leuconostoc mesenteroides | |
0.233 | - |
NAD+ | mutant enzyme R46Q, in 33 mM Tris-HCl, pH 7.6, at 25°C | Leuconostoc mesenteroides | |
0.334 | - |
D-glucose 6-phosphate | 25°C, pH 7.6, mutant enzyme R46Q | Leuconostoc mesenteroides | |
0.334 | - |
D-glucose 6-phosphate | mutant enzyme R46Q, with NADP+ as cosubstrate, in 33 mM Tris-HCl, pH 7.6, at 25°C | Leuconostoc mesenteroides | |
0.467 | - |
D-glucose 6-phosphate | 25°C, pH 7.6, mutant | Leuconostoc mesenteroides | |
0.467 | - |
D-glucose 6-phosphate | mutant enzyme R46A, with NADP+ as cosubstrate, in 33 mM Tris-HCl, pH 7.6, at 25°C | Leuconostoc mesenteroides | |
0.854 | - |
NADP+ | 25°C, pH 7.6, mutant enzyme R46Q | Leuconostoc mesenteroides | |
0.854 | - |
NADP+ | mutant enzyme R46Q, in 33 mM Tris-HCl, pH 7.6, at 25°C | Leuconostoc mesenteroides | |
1.05 | - |
NADP+ | 25°C, pH 7.6, mutant enzyme R46A | Leuconostoc mesenteroides | |
1.05 | - |
NAD+ | mutant enzyme R46A, in 33 mM Tris-HCl, pH 7.6, at 25°C | Leuconostoc mesenteroides | |
5.57 | - |
NADP+ | mutant enzyme R46A, in 33 mM Tris-HCl, pH 7.6, at 25°C | Leuconostoc mesenteroides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leuconostoc mesenteroides | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + NAD(P)+ | - |
Leuconostoc mesenteroides | 6-phospho-D-glucono-1,5-lactone + NAD(P)H + H+ | - |
? | |
D-glucose 6-phosphate + NAD+ | - |
Leuconostoc mesenteroides | 6-phospho-D-glucono-1,5-lactone + NADH + H+ | - |
? | |
D-glucose 6-phosphate + NADP+ | - |
Leuconostoc mesenteroides | 6-phospho-D-glucono-1,5-lactone + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Leuconostoc mesenteroides |
Synonyms | Comment | Organism |
---|---|---|
G6PD | - |
Leuconostoc mesenteroides |
glucose-6-phosphate dehydrogenase | - |
Leuconostoc mesenteroides |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Leuconostoc mesenteroides |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
522 | - |
D-glucose 6-phosphate | wild type enzyme, with NADP+ as cosubstrate, in 33 mM Tris-HCl, pH 7.6, at 25°C | Leuconostoc mesenteroides | |
825 | - |
NADP+ | 25°C, pH 7.6, mutant enzyme R46Q | Leuconostoc mesenteroides | |
825 | - |
D-glucose 6-phosphate | 25°C, pH 7.6, mutant enzyme R46Q | Leuconostoc mesenteroides | |
825 | - |
D-glucose 6-phosphate | mutant enzyme R46Q, with NAD+ as cosubstrate, in 33 mM Tris-HCl, pH 7.6, at 25°C | Leuconostoc mesenteroides | |
887 | - |
NADP+ | 25°C, pH 7.6, mutant enzyme R46Q | Leuconostoc mesenteroides | |
887 | - |
D-glucose 6-phosphate | 25°C, pH 7.6, mutant enzyme R46Q | Leuconostoc mesenteroides | |
887 | - |
D-glucose 6-phosphate | mutant enzyme R46Q, with NADP+ as cosubstrate, in 33 mM Tris-HCl, pH 7.6, at 25°C | Leuconostoc mesenteroides | |
1125 | - |
D-glucose 6-phosphate | wild type enzyme, with NAD+ as cosubstrate, in 33 mM Tris-HCl, pH 7.6, at 25°C | Leuconostoc mesenteroides | |
1535 | - |
NADP+ | 25°C, pH 7.6, mutant enzyme R46A | Leuconostoc mesenteroides | |
1535 | - |
D-glucose 6-phosphate | 25°C, pH 7.6, mutant enzyme R46A | Leuconostoc mesenteroides | |
1535 | - |
D-glucose 6-phosphate | mutant enzyme R46A, with NAD+ as cosubstrate, in 33 mM Tris-HCl, pH 7.6, at 25°C | Leuconostoc mesenteroides | |
1800 | - |
NADP+ | 25°C, pH 7.6, mutant enzyme R46A | Leuconostoc mesenteroides | |
1800 | - |
D-glucose 6-phosphate | 25°C, pH 7.6, mutant enzyme R46A | Leuconostoc mesenteroides | |
1800 | - |
D-glucose 6-phosphate | mutant enzyme R46A, with NADP+ as cosubstrate, in 33 mM Tris-HCl, pH 7.6, at 25°C | Leuconostoc mesenteroides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Leuconostoc mesenteroides |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | can utilize either NADP+ or NAD+ as coenzyme | Leuconostoc mesenteroides | |
NAD+ | wild-type enzyme prefers NADP+ as coenzyme | Leuconostoc mesenteroides | |
NADP+ | can utilize either NADP+ or NAD+ as coenzyme | Leuconostoc mesenteroides | |
NADP+ | wild-type enzyme prefers NADP+ as coenzyme. Arg46 plays a key role in NADP+-binding | Leuconostoc mesenteroides |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00337 | - |
NADP+ | wild type enzyme, in 33 mM Tris-HCl, pH 7.6, at 25°C | Leuconostoc mesenteroides | |
1.79 | - |
NADP+ | mutant enzyme R46Q, in 33 mM Tris-HCl, pH 7.6, at 25°C | Leuconostoc mesenteroides | |
1.8 | - |
NADP+ | 25°C, pH 7.6, mutant enzyme R46Q | Leuconostoc mesenteroides | |
8.23 | - |
NADP+ | 25°C, pH 7.6, mutant enzyme R46A | Leuconostoc mesenteroides | |
8.23 | - |
NADP+ | mutant enzyme R46A, in 33 mM Tris-HCl, pH 7.6, at 25°C | Leuconostoc mesenteroides |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.57 | - |
NADP+ | mutant enzyme R46A, in 33 mM Tris-HCl, pH 7.6, at 25°C | Leuconostoc mesenteroides | |
323 | - |
NADP+ | 25°C, pH 7.6, mutant enzyme R46A | Leuconostoc mesenteroides | |
323 | - |
NADP+ | mutant enzyme R46Q, in 33 mM Tris-HCl, pH 7.6, at 25°C | Leuconostoc mesenteroides | |
1039 | - |
NADP+ | 25°C, pH 7.6, mutant enzyme R46Q | Leuconostoc mesenteroides | |
1462 | - |
NADP+ | 25°C, pH 7.6, mutant enzyme R46A | Leuconostoc mesenteroides | |
1462 | - |
NAD+ | mutant enzyme R46A, in 33 mM Tris-HCl, pH 7.6, at 25°C | Leuconostoc mesenteroides | |
2650 | - |
D-glucose 6-phosphate | mutant enzyme R46Q, with NADP+ as cosubstrate, in 33 mM Tris-HCl, pH 7.6, at 25°C | Leuconostoc mesenteroides | |
2656 | - |
D-glucose 6-phosphate | 25°C, pH 7.6, mutant enzyme R46Q | Leuconostoc mesenteroides | |
3533 | - |
NAD+ | mutant enzyme R46Q, in 33 mM Tris-HCl, pH 7.6, at 25°C | Leuconostoc mesenteroides | |
3540 | - |
NADP+ | 25°C, pH 7.6, mutant enzyme R46Q | Leuconostoc mesenteroides | |
3850 | - |
D-glucose 6-phosphate | mutant enzyme R46A, with NADP+ as cosubstrate, in 33 mM Tris-HCl, pH 7.6, at 25°C | Leuconostoc mesenteroides | |
3854 | - |
D-glucose 6-phosphate | 25°C, pH 7.6, mutant enzyme R46A | Leuconostoc mesenteroides | |
4583 | - |
D-glucose 6-phosphate | wild type enzyme, with NADP+ as cosubstrate, in 33 mM Tris-HCl, pH 7.6, at 25°C | Leuconostoc mesenteroides | |
6950 | - |
NAD+ | wild type enzyme, in 33 mM Tris-HCl, pH 7.6, at 25°C | Leuconostoc mesenteroides | |
8767 | - |
D-glucose 6-phosphate | mutant enzyme R46A, with NAD+ as cosubstrate, in 33 mM Tris-HCl, pH 7.6, at 25°C | Leuconostoc mesenteroides | |
8771 | - |
D-glucose 6-phosphate | 25°C, pH 7.6, mutant enzyme R46A | Leuconostoc mesenteroides | |
9267 | - |
D-glucose 6-phosphate | mutant enzyme R46Q, with NAD+ as cosubstrate, in 33 mM Tris-HCl, pH 7.6, at 25°C | Leuconostoc mesenteroides | |
9270 | - |
D-glucose 6-phosphate | 25°C, pH 7.6, mutant enzyme R46Q | Leuconostoc mesenteroides | |
16250 | - |
D-glucose 6-phosphate | wild type enzyme, with NAD+ as cosubstrate, in 33 mM Tris-HCl, pH 7.6, at 25°C | Leuconostoc mesenteroides | |
65330 | - |
NADP+ | wild type enzyme, in 33 mM Tris-HCl, pH 7.6, at 25°C | Leuconostoc mesenteroides |