Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Leuconostoc mesenteroides |
Protein Variants | Comment | Organism |
---|---|---|
K21Q | kcat/Km for D-glucose 6-phosphate in the NADP+-dependent reaction is 76.2fold lower compared to wild-type value, kcat/Km for D-glucose 6-phosphate in the NAD+-dependent reaction is 28.5fold lower compared to wild-type value | Leuconostoc mesenteroides |
K21R | kcat/Km for D-glucose 6-phosphate in the NADP+-dependent reaction is 1.9fold lower compared to wild-type value, kcat/Km for D-glucose 6-phosphate in the NAD+-dependent reaction is 1.4fold lower compared to wild-type value | Leuconostoc mesenteroides |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Leuconostoc mesenteroides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
NADP+ | 25°C, pH 7.6, mutant enzyme K21Q | Leuconostoc mesenteroides | |
0.008 | - |
NADP+ | 25°C, pH 7.6, wild-type enzyme | Leuconostoc mesenteroides | |
0.008 | - |
NADP+ | 25°C, pH 7.6, mutant enzyme K21R | Leuconostoc mesenteroides | |
0.069 | - |
D-glucose 6-phosphate | 25°C, pH 7.6, cosubstrate: NAD+, wild-type enzyme | Leuconostoc mesenteroides | |
0.097 | - |
D-glucose 6-phosphate | 25°C, pH 7.6, cosubstrate: NAD+, mutant enzyme K21R | Leuconostoc mesenteroides | |
0.114 | - |
D-glucose 6-phosphate | 25°C, pH 7.6, cosubstrate: NADP+, wild-type enzyme | Leuconostoc mesenteroides | |
0.16 | - |
NAD+ | 25°C, pH 7.6, wild-type enzyme | Leuconostoc mesenteroides | |
0.239 | - |
D-glucose 6-phosphate | 25°C, pH 7.6, cosubstrate: NADP+, mutant enzyme K21R | Leuconostoc mesenteroides | |
0.24 | - |
NAD+ | 25°C, pH 7.6, mutant enzyme K21R | Leuconostoc mesenteroides | |
0.52 | - |
NAD+ | 25°C, pH 7.6, mutant enzyme K21Q | Leuconostoc mesenteroides | |
2.5 | - |
D-glucose 6-phosphate | 25°C, pH 7.6, cosubstrate: NAD+, mutant enzyme K21Q | Leuconostoc mesenteroides | |
6.07 | - |
D-glucose 6-phosphate | 25°C, pH 7.6, cosubstrate: NADP+, mutant enzyme K21Q | Leuconostoc mesenteroides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + NAD+ | Leuconostoc mesenteroides | - |
6-phospho-D-glucono-1,5-lactone + NADH + H+ | - |
? | |
D-glucose 6-phosphate + NADP+ | Leuconostoc mesenteroides | - |
6-phospho-D-glucono-1,5-lactone + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leuconostoc mesenteroides | P11411 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Leuconostoc mesenteroides |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
715 | - |
25°C, pH 7.6, wild-type enzyme | Leuconostoc mesenteroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + NAD+ | - |
Leuconostoc mesenteroides | 6-phospho-D-glucono-1,5-lactone + NADH + H+ | - |
? | |
D-glucose 6-phosphate + NADP+ | - |
Leuconostoc mesenteroides | 6-phospho-D-glucono-1,5-lactone + NADPH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
G6PD | - |
Leuconostoc mesenteroides |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Leuconostoc mesenteroides |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
100 | - |
D-glucose 6-phosphate | 25°C, pH 7.6, cosubstrate: NADP+, mutant enzyme K21Q | Leuconostoc mesenteroides | |
100 | - |
NADP+ | 25°C, pH 7.6, mutant enzyme K21Q | Leuconostoc mesenteroides | |
365 | - |
D-glucose 6-phosphate | 25°C, pH 7.6, cosubstrate: NAD+, mutant enzyme K21R | Leuconostoc mesenteroides | |
365 | - |
NAD+ | 25°C, pH 7.6, mutant enzyme K21R | Leuconostoc mesenteroides | |
522 | - |
NADP+ | 25°C, pH 7.6, wild-type enzyme | Leuconostoc mesenteroides | |
522 | - |
D-glucose 6-phosphate | 25°C, pH 7.6, cosubstrate: NADP+, wild-type enzyme | Leuconostoc mesenteroides | |
582 | - |
D-glucose 6-phosphate | 25°C, pH 7.6, cosubstrate: NADP+, mutant enzyme K21R | Leuconostoc mesenteroides | |
582 | - |
NADP+ | 25°C, pH 7.6, mutant enzyme K21R | Leuconostoc mesenteroides | |
1125 | - |
NAD+ | 25°C, pH 7.6, wild-type enzyme | Leuconostoc mesenteroides | |
1125 | - |
D-glucose 6-phosphate | 25°C, pH 7.6, cosubstrate: NAD+, wild-type enzyme | Leuconostoc mesenteroides | |
1423 | - |
D-glucose 6-phosphate | 25°C, pH 7.6, cosubstrate: NAD+, mutant enzyme K21Q | Leuconostoc mesenteroides | |
1423 | - |
NAD+ | 25°C, pH 7.6, mutant enzyme K21Q | Leuconostoc mesenteroides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Leuconostoc mesenteroides |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | kcat/Km of NADP+ is 9.3fold higher compared to kcat/Km of D-glucose 6-phosphate in NAD+-dependent reaction, kcat/Km of D-glucose 6-phosphate in the NADP+-dependent reaction is 3.5fold higher compared to kcat/Km of D-glucose 6-phosphate in NAD+-dependent reaction. kcat/Km of D-glucose 6-phosphate in the NADP+-dependent reaction is 3.5fold higher compared to kcat/Km of D-glucose 6-phosphate in NAD+-dependent reaction | Leuconostoc mesenteroides | |
NADP+ | kcat/Km of NADP+ is 9.3fold higher compared to kcat/Km of D-glucose 6-phosphate in NAD+-dependent reaction, kcat/Km of D-glucose 6-phosphate in the NADP+-dependent reaction is 3.5fold higher compared to kcat/Km of D-glucose 6-phosphate in NAD+-dependent reaction. kcat/Km of D-glucose 6-phosphate in the NADP+-dependent reaction is 3.5fold higher compared to kcat/Km of D-glucose 6-phosphate in NAD+-dependent reaction | Leuconostoc mesenteroides |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0034 | - |
NADP+ | 25°C, pH 7.6, wild-type enzyme | Leuconostoc mesenteroides | |
0.0062 | - |
NADP+ | 25°C, pH 7.6, wild-type enzyme, mutant enzyme K121R | Leuconostoc mesenteroides | |
0.0078 | - |
NADP+ | 25°C, pH 7.6, wild-type enzyme, mutant enzyme K21Q | Leuconostoc mesenteroides |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
16.5 | - |
D-glucose 6-phosphate | 25°C, pH 7.6, cosubstrate: NADP+, mutant enzyme K21Q | Leuconostoc mesenteroides | |
569 | - |
D-glucose 6-phosphate | 25°C, pH 7.6, cosubstrate: NAD+, mutant enzyme K21Q | Leuconostoc mesenteroides | |
2435 | - |
D-glucose 6-phosphate | 25°C, pH 7.6, cosubstrate: NADP+, mutant enzyme K21R | Leuconostoc mesenteroides | |
2956 | - |
D-glucose 6-phosphate | 25°C, pH 7.6, cosubstrate: NADP+, wild-type enzyme | Leuconostoc mesenteroides | |
3762 | - |
D-glucose 6-phosphate | 25°C, pH 7.6, cosubstrate: NAD+, mutant enzyme K21R | Leuconostoc mesenteroides | |
7031 | - |
NAD+ | 25°C, pH 7.6, wild-type enzyme | Leuconostoc mesenteroides | |
10540 | - |
D-glucose 6-phosphate | 25°C, pH 7.6, cosubstrate: NAD+, wild-type enzyme | Leuconostoc mesenteroides | |
65250 | - |
NADP+ | 25°C, pH 7.6, wild-type enzyme | Leuconostoc mesenteroides |