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Literature summary for 1.1.1.363 extracted from

  • Lee, W.T.; Levy, H.R.
    Lysine-21 of Leuconostoc mesenteroides glucose 6-phosphate dehydrogenase participates in substrate binding through charge-charge interaction (1992), Protein Sci., 1, 329-334.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Leuconostoc mesenteroides

Protein Variants

Protein Variants Comment Organism
K21Q kcat/Km for D-glucose 6-phosphate in the NADP+-dependent reaction is 76.2fold lower compared to wild-type value, kcat/Km for D-glucose 6-phosphate in the NAD+-dependent reaction is 28.5fold lower compared to wild-type value Leuconostoc mesenteroides
K21R kcat/Km for D-glucose 6-phosphate in the NADP+-dependent reaction is 1.9fold lower compared to wild-type value, kcat/Km for D-glucose 6-phosphate in the NAD+-dependent reaction is 1.4fold lower compared to wild-type value Leuconostoc mesenteroides

Inhibitors

Inhibitors Comment Organism Structure
NADP+
-
Leuconostoc mesenteroides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
NADP+ 25°C, pH 7.6, mutant enzyme K21Q Leuconostoc mesenteroides
0.008
-
NADP+ 25°C, pH 7.6, wild-type enzyme Leuconostoc mesenteroides
0.008
-
NADP+ 25°C, pH 7.6, mutant enzyme K21R Leuconostoc mesenteroides
0.069
-
D-glucose 6-phosphate 25°C, pH 7.6, cosubstrate: NAD+, wild-type enzyme Leuconostoc mesenteroides
0.097
-
D-glucose 6-phosphate 25°C, pH 7.6, cosubstrate: NAD+, mutant enzyme K21R Leuconostoc mesenteroides
0.114
-
D-glucose 6-phosphate 25°C, pH 7.6, cosubstrate: NADP+, wild-type enzyme Leuconostoc mesenteroides
0.16
-
NAD+ 25°C, pH 7.6, wild-type enzyme Leuconostoc mesenteroides
0.239
-
D-glucose 6-phosphate 25°C, pH 7.6, cosubstrate: NADP+, mutant enzyme K21R Leuconostoc mesenteroides
0.24
-
NAD+ 25°C, pH 7.6, mutant enzyme K21R Leuconostoc mesenteroides
0.52
-
NAD+ 25°C, pH 7.6, mutant enzyme K21Q Leuconostoc mesenteroides
2.5
-
D-glucose 6-phosphate 25°C, pH 7.6, cosubstrate: NAD+, mutant enzyme K21Q Leuconostoc mesenteroides
6.07
-
D-glucose 6-phosphate 25°C, pH 7.6, cosubstrate: NADP+, mutant enzyme K21Q Leuconostoc mesenteroides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucose 6-phosphate + NAD+ Leuconostoc mesenteroides
-
6-phospho-D-glucono-1,5-lactone + NADH + H+
-
?
D-glucose 6-phosphate + NADP+ Leuconostoc mesenteroides
-
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
?

Organism

Organism UniProt Comment Textmining
Leuconostoc mesenteroides P11411
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Leuconostoc mesenteroides

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
715
-
25°C, pH 7.6, wild-type enzyme Leuconostoc mesenteroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose 6-phosphate + NAD+
-
Leuconostoc mesenteroides 6-phospho-D-glucono-1,5-lactone + NADH + H+
-
?
D-glucose 6-phosphate + NADP+
-
Leuconostoc mesenteroides 6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
?

Synonyms

Synonyms Comment Organism
G6PD
-
Leuconostoc mesenteroides

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Leuconostoc mesenteroides

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
100
-
D-glucose 6-phosphate 25°C, pH 7.6, cosubstrate: NADP+, mutant enzyme K21Q Leuconostoc mesenteroides
100
-
NADP+ 25°C, pH 7.6, mutant enzyme K21Q Leuconostoc mesenteroides
365
-
D-glucose 6-phosphate 25°C, pH 7.6, cosubstrate: NAD+, mutant enzyme K21R Leuconostoc mesenteroides
365
-
NAD+ 25°C, pH 7.6, mutant enzyme K21R Leuconostoc mesenteroides
522
-
NADP+ 25°C, pH 7.6, wild-type enzyme Leuconostoc mesenteroides
522
-
D-glucose 6-phosphate 25°C, pH 7.6, cosubstrate: NADP+, wild-type enzyme Leuconostoc mesenteroides
582
-
D-glucose 6-phosphate 25°C, pH 7.6, cosubstrate: NADP+, mutant enzyme K21R Leuconostoc mesenteroides
582
-
NADP+ 25°C, pH 7.6, mutant enzyme K21R Leuconostoc mesenteroides
1125
-
NAD+ 25°C, pH 7.6, wild-type enzyme Leuconostoc mesenteroides
1125
-
D-glucose 6-phosphate 25°C, pH 7.6, cosubstrate: NAD+, wild-type enzyme Leuconostoc mesenteroides
1423
-
D-glucose 6-phosphate 25°C, pH 7.6, cosubstrate: NAD+, mutant enzyme K21Q Leuconostoc mesenteroides
1423
-
NAD+ 25°C, pH 7.6, mutant enzyme K21Q Leuconostoc mesenteroides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
assay at Leuconostoc mesenteroides

Cofactor

Cofactor Comment Organism Structure
NAD+ kcat/Km of NADP+ is 9.3fold higher compared to kcat/Km of D-glucose 6-phosphate in NAD+-dependent reaction, kcat/Km of D-glucose 6-phosphate in the NADP+-dependent reaction is 3.5fold higher compared to kcat/Km of D-glucose 6-phosphate in NAD+-dependent reaction. kcat/Km of D-glucose 6-phosphate in the NADP+-dependent reaction is 3.5fold higher compared to kcat/Km of D-glucose 6-phosphate in NAD+-dependent reaction Leuconostoc mesenteroides
NADP+ kcat/Km of NADP+ is 9.3fold higher compared to kcat/Km of D-glucose 6-phosphate in NAD+-dependent reaction, kcat/Km of D-glucose 6-phosphate in the NADP+-dependent reaction is 3.5fold higher compared to kcat/Km of D-glucose 6-phosphate in NAD+-dependent reaction. kcat/Km of D-glucose 6-phosphate in the NADP+-dependent reaction is 3.5fold higher compared to kcat/Km of D-glucose 6-phosphate in NAD+-dependent reaction Leuconostoc mesenteroides

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0034
-
NADP+ 25°C, pH 7.6, wild-type enzyme Leuconostoc mesenteroides
0.0062
-
NADP+ 25°C, pH 7.6, wild-type enzyme, mutant enzyme K121R Leuconostoc mesenteroides
0.0078
-
NADP+ 25°C, pH 7.6, wild-type enzyme, mutant enzyme K21Q Leuconostoc mesenteroides

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
16.5
-
D-glucose 6-phosphate 25°C, pH 7.6, cosubstrate: NADP+, mutant enzyme K21Q Leuconostoc mesenteroides
569
-
D-glucose 6-phosphate 25°C, pH 7.6, cosubstrate: NAD+, mutant enzyme K21Q Leuconostoc mesenteroides
2435
-
D-glucose 6-phosphate 25°C, pH 7.6, cosubstrate: NADP+, mutant enzyme K21R Leuconostoc mesenteroides
2956
-
D-glucose 6-phosphate 25°C, pH 7.6, cosubstrate: NADP+, wild-type enzyme Leuconostoc mesenteroides
3762
-
D-glucose 6-phosphate 25°C, pH 7.6, cosubstrate: NAD+, mutant enzyme K21R Leuconostoc mesenteroides
7031
-
NAD+ 25°C, pH 7.6, wild-type enzyme Leuconostoc mesenteroides
10540
-
D-glucose 6-phosphate 25°C, pH 7.6, cosubstrate: NAD+, wild-type enzyme Leuconostoc mesenteroides
65250
-
NADP+ 25°C, pH 7.6, wild-type enzyme Leuconostoc mesenteroides