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Literature summary for 1.1.1.363 extracted from

  • Olavarria, K.; Marone, M.P.; da Costa Oliveira, H.; Roncallo, J.C.; da Costa Vasconcelos, F.N.; da Silva, L.F.; Gomez, J.G.
    Quantifying NAD(P)H production in the upper Entner-Doudoroff pathway from Pseudomonas putida KT2440 (2015), FEBS open bio, 5, 908-915 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene zwf-1, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) and strain MG1655. The His-tagged form of PputG6PDH-1 shows no significant differences with respect to the non-tagged form of the same enzyme Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
NAD+
-
Pseudomonas putida
NADH competitive inhibition Pseudomonas putida
NADP+ mixed-type inhibition Pseudomonas putida
NADPH inhibits competitively when NADP+ is the varying substrate and noncompetitively when D-glucose 6-phosphate is the varying substrate Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics and modelling, detailed overview Pseudomonas putida
0.014
-
NADP+ pH 8.0, 30°C, recombinant enzyme Pseudomonas putida
0.127
-
NAD+ pH 8.0, 30°C, recombinant enzyme Pseudomonas putida
0.946
-
D-glucose 6-phosphate pH 8.0, 30°C, recombinant enzyme, with NADP+ Pseudomonas putida
1.137
-
D-glucose 6-phosphate pH 8.0, 30°C, recombinant enzyme, with NAD+ Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucose 6-phosphate + NAD+ Pseudomonas putida
-
6-phospho-D-glucono-1,5-lactone + NADH + H+
-
r
D-glucose 6-phosphate + NAD+ Pseudomonas putida KT 2240
-
6-phospho-D-glucono-1,5-lactone + NADH + H+
-
r
D-glucose 6-phosphate + NADP+ Pseudomonas putida
-
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
r
D-glucose 6-phosphate + NADP+ Pseudomonas putida KT 2240
-
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
r
additional information Pseudomonas putida the reaction catalyzed by PputG6PDH-1 yields around 1/3 mol of NADPH and 2/3 mol of NADH per mol of oxidized glucose 6-phosphate, calculation of in vivo relative production of NADH and NADPH during the oxidation of glucose-6-phosphate. The reaction catalyzes by the 6-phosphogluconate dehydrogenase is active during growth on glucose, and it produces NADPH and NADH. In comparison, for the enzyme of Escherichia coli EcG6PDH, the relative production of NADH over NADPH is 1000times lower than for PputG6PDH-1 ?
-
?
additional information Pseudomonas putida KT 2240 the reaction catalyzed by PputG6PDH-1 yields around 1/3 mol of NADPH and 2/3 mol of NADH per mol of oxidized glucose 6-phosphate, calculation of in vivo relative production of NADH and NADPH during the oxidation of glucose-6-phosphate. The reaction catalyzes by the 6-phosphogluconate dehydrogenase is active during growth on glucose, and it produces NADPH and NADH. In comparison, for the enzyme of Escherichia coli EcG6PDH, the relative production of NADH over NADPH is 1000times lower than for PputG6PDH-1 ?
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida A0A1B2F298
-
-
Pseudomonas putida KT 2240 A0A1B2F298
-
-

Reaction

Reaction Comment Organism Reaction ID
D-glucose 6-phosphate + NAD(P)+ = 6-phospho-D-glucono-1,5-lactone + NAD(P)H + H+ enzyme PputG6PDH-1 from Pseudomonas putida KT2440 catalyzes the oxidation of D-glucose 6-phosphate using a rapidequilibrium random mechanism Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
the His-tagged form of PputG6PDH-1 shows no significant differences with respect to the non-tagged form of the same enzyme Pseudomonas putida
0.006
-
enzyme in Pseudomonas putida KT2440 crude cell extract, pH 8.0, 30°C, with NADP+ Pseudomonas putida
0.008
-
recombinant enzyme in Escherichia coli MG1655 crude cell extract, pH 8.0, 30°C, with NAD+ Pseudomonas putida
0.025
-
enzyme in Pseudomonas putida KT2440 crude cell extract, pH 8.0, 30°C, with NAD+ Pseudomonas putida
0.103
-
recombinant enzyme in Escherichia coli MG1655 crude cell extract, pH 8.0, 30°C, with NADP+ Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-phospho-D-glucono-1,5-lactone + NADH + H+
-
Pseudomonas putida D-glucose 6-phosphate + NAD+
-
r
6-phospho-D-glucono-1,5-lactone + NADH + H+
-
Pseudomonas putida KT 2240 D-glucose 6-phosphate + NAD+
-
r
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
Pseudomonas putida D-glucose 6-phosphate + NADP+
-
r
D-glucose 6-phosphate + NAD+
-
Pseudomonas putida 6-phospho-D-glucono-1,5-lactone + NADH + H+
-
r
D-glucose 6-phosphate + NAD+
-
Pseudomonas putida KT 2240 6-phospho-D-glucono-1,5-lactone + NADH + H+
-
r
D-glucose 6-phosphate + NADP+
-
Pseudomonas putida 6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
r
D-glucose 6-phosphate + NADP+
-
Pseudomonas putida KT 2240 6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
r
additional information the reaction catalyzed by PputG6PDH-1 yields around 1/3 mol of NADPH and 2/3 mol of NADH per mol of oxidized glucose 6-phosphate, calculation of in vivo relative production of NADH and NADPH during the oxidation of glucose-6-phosphate. The reaction catalyzes by the 6-phosphogluconate dehydrogenase is active during growth on glucose, and it produces NADPH and NADH. In comparison, for the enzyme of Escherichia coli EcG6PDH, the relative production of NADH over NADPH is 1000times lower than for PputG6PDH-1 Pseudomonas putida ?
-
?
additional information the reaction catalyzed by PputG6PDH-1 yields around 1/3 mol of NADPH and 2/3 mol of NADH per mol of oxidized glucose 6-phosphate. PputG6PDH-1 is not considered a NADP+-specific enzyme Pseudomonas putida ?
-
?
additional information the reaction catalyzed by PputG6PDH-1 yields around 1/3 mol of NADPH and 2/3 mol of NADH per mol of oxidized glucose 6-phosphate, calculation of in vivo relative production of NADH and NADPH during the oxidation of glucose-6-phosphate. The reaction catalyzes by the 6-phosphogluconate dehydrogenase is active during growth on glucose, and it produces NADPH and NADH. In comparison, for the enzyme of Escherichia coli EcG6PDH, the relative production of NADH over NADPH is 1000times lower than for PputG6PDH-1 Pseudomonas putida KT 2240 ?
-
?
additional information the reaction catalyzed by PputG6PDH-1 yields around 1/3 mol of NADPH and 2/3 mol of NADH per mol of oxidized glucose 6-phosphate. PputG6PDH-1 is not considered a NADP+-specific enzyme Pseudomonas putida KT 2240 ?
-
?

Synonyms

Synonyms Comment Organism
glucose-6-phosphate dehydrogenase
-
Pseudomonas putida
PputG6PDH-1
-
Pseudomonas putida
zwf-1
-
Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Pseudomonas putida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
102
-
NADP+ pH 8.0, 30°C, recombinant enzyme Pseudomonas putida
105
-
NADPH pH 8.0, 30°C, recombinant enzyme Pseudomonas putida
254
-
NADH pH 8.0, 30°C, recombinant enzyme Pseudomonas putida
277
-
NAD+ pH 8.0, 30°C, recombinant enzyme Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Pseudomonas putida
NADH
-
Pseudomonas putida
NADP+ preferred cofactor Pseudomonas putida
NADPH
-
Pseudomonas putida

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.035 0.163 NADPH pH 8.0, 30°C, recombinant enzyme Pseudomonas putida
0.111
-
NADP+ pH 8.0, 30°C, recombinant enzyme Pseudomonas putida
1.148
-
NAD+ pH 8.0, 30°C, recombinant enzyme Pseudomonas putida

General Information

General Information Comment Organism
additional information the His-tagged form of PputG6PDH-1 shows no significant differences with respect to the non-tagged form of the same enzyme Pseudomonas putida

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2181
-
NAD+ pH 8.0, 30°C, recombinant enzyme Pseudomonas putida
7286
-
NADP+ pH 8.0, 30°C, recombinant enzyme Pseudomonas putida