Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 | Streptomyces sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Streptomyces sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
x * 52000, including the 6His-tag and thioredoxin-tagged fusion protein, SDS-PAGE | Streptomyces sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTDP-4-dehydro-6-deoxy-alpha-D-gulopyranose + NADH + H+ | Streptomyces sp. | the enzyme forms an activated deoxy-alpha-D-allose, which is converted to mycinose after attachment to the aglycones of several macrolide antibiotics, including tylosin, chalcomycin, dihydrochalcomycin, and mycinamicin II | dTDP-6-deoxy-alpha-D-allopyranose + NAD+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. | Q331Q7 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Streptomyces sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTDP-4-dehydro-6-deoxy-alpha-D-gulopyranose + NADH + H+ | the enzyme forms an activated deoxy-alpha-D-allose, which is converted to mycinose after attachment to the aglycones of several macrolide antibiotics, including tylosin, chalcomycin, dihydrochalcomycin, and mycinamicin II | Streptomyces sp. | dTDP-6-deoxy-alpha-D-allopyranose + NAD+ | - |
? | |
dTDP-4-dehydro-6-deoxy-alpha-D-gulopyranose + NADH + H+ | dTDP-4-dehydro-6-deoxy-alpha-D-gulose i.e. dTDP-4-dehydro-6-deoxy-alpha-D-allose | Streptomyces sp. | dTDP-6-deoxy-alpha-D-allopyranose + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 52000, including the 6His-tag and thioredoxin-tagged fusion protein, SDS-PAGE | Streptomyces sp. |
Synonyms | Comment | Organism |
---|---|---|
GerKI | - |
Streptomyces sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Streptomyces sp. |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme forms an activated deoxy-alpha-D-allose, which is converted to mycinose after attachment to the aglycones of several macrolide antibiotics, including tylosin, chalcomycin, dihydrochalcomycin, and mycinamicin II | Streptomyces sp. |