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Literature summary for 1.1.1.37 extracted from
- Purification and properties of cytoplasmic and mitochondria malate dehydrogenase of Physarum polycephalum (1973), J. Bacteriol., 116, 673-684.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| L-malate | - |
Gallus sp. |  |
| L-malate | - |
Physarum polycephalum | |
| oxaloacetate | - |
Drosophila virilis | |
| oxaloacetate | - |
Phycomyces blakesleeanus | |
| oxaloacetate | - |
Physarum polycephalum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.048 | - |
NADH | s-MDH | Physarum polycephalum | |
| 0.06 | - |
oxaloacetate | s-MDH | Physarum polycephalum | |
| 0.074 | - |
oxaloacetate | m-MDH | Physarum polycephalum | |
| 0.19 | - |
NAD+ | m-MDH | Physarum polycephalum | |
| 0.2 | - |
NADH | m-MDH | Physarum polycephalum | |
| 0.75 | - |
NAD+ | s-MDH | Physarum polycephalum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Saccharomyces cerevisiae | 5737 | - |
| cytoplasm | - |
Bos taurus | 5737 | - |
| cytoplasm | - |
Neurospora crassa | 5737 | - |
| cytoplasm | - |
Phycomyces blakesleeanus | 5737 | - |
| cytoplasm | - |
Spinacia oleracea | 5737 | - |
| cytoplasm | - |
Ascaris suum | 5737 | - |
| cytoplasm | - |
Physarum polycephalum | 5737 | - |
| cytoplasm | - |
Fundulus heteroclitus | 5737 | - |
| cytoplasm | - |
Gallus sp. | 5737 | - |
| cytoplasm | - |
Drosophila virilis | 5737 | - |
| cytoplasm | - |
Thunnus albacares | 5737 | - |
| cytosol | - |
Saccharomyces cerevisiae | 5829 | - |
| cytosol | - |
Bos taurus | 5829 | - |
| cytosol | - |
Physarum polycephalum | 5829 | - |
| cytosol | - |
Gallus sp. | 5829 | - |
| mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
| mitochondrion | - |
Bos taurus | 5739 | - |
| mitochondrion | - |
Neurospora crassa | 5739 | - |
| mitochondrion | - |
Spinacia oleracea | 5739 | - |
| mitochondrion | - |
Ascaris suum | 5739 | - |
| mitochondrion | - |
Physarum polycephalum | 5739 | - |
| mitochondrion | - |
Fundulus heteroclitus | 5739 | - |
| mitochondrion | - |
Gallus sp. | 5739 | - |
| mitochondrion | - |
Drosophila virilis | 5739 | - |
| mitochondrion | - |
Thunnus albacares | 5739 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| malate + NAD+ | Saccharomyces cerevisiae | - |
oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | Bos taurus | - |
oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | Neurospora crassa | - |
oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | Phycomyces blakesleeanus | - |
oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | Spinacia oleracea | - |
oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | Zea mays | - |
oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | Ascaris suum | - |
oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | Auxenochlorella pyrenoidosa | - |
oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | Physarum polycephalum | - |
oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | Citrus sp. | - |
oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | Strongylocentrotus purpuratus | - |
oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | Fundulus heteroclitus | - |
oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | Gallus sp. | - |
oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | Drosophila virilis | - |
oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | Thunnus albacares | - |
oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | Opuntia ficus-indica | - |
oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | Salmo sp. | - |
oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | Tritia obsoleta | - |
oxaloacetate + NADH + H+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ascaris suum | - |
- |
- |
| Auxenochlorella pyrenoidosa | - |
- |
- |
| Bos taurus | - |
- |
- |
| Citrus sp. | - |
lemon fruit | - |
| Drosophila virilis | - |
- |
- |
| Fundulus heteroclitus | - |
teleost | - |
| Gallus sp. | - |
chicken | - |
| Neurospora crassa | - |
- |
- |
| Opuntia ficus-indica | - |
cactus | - |
| Phycomyces blakesleeanus | - |
- |
- |
| Physarum polycephalum | - |
- |
- |
| Saccharomyces cerevisiae | - |
yeast | - |
| Salmo sp. | - |
salmonid fish | - |
| Spinacia oleracea | - |
spinach | - |
| Strongylocentrotus purpuratus | - |
sea urchin | - |
| Thunnus albacares | - |
tuna | - |
| Tritia obsoleta | - |
marine snail | - |
| Zea mays | - |
maize | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 2 isoenzymes, S-MDH and M-MDH | Physarum polycephalum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| endosperm | - |
Zea mays | - |
| kidney | - |
Bos taurus | - |
| leaf | - |
Spinacia oleracea | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 774 | - |
- |
Physarum polycephalum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, purified enzymes are stable for up to 2 months in 0.05 M potassium phosphate buffer, pH 7.5 containing 0.001 M 2-mercaptoethanol | Physarum polycephalum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate + NAD+ | - |
Saccharomyces cerevisiae | oxaloacetate + NADH + H+ | - |
r | |
| (S)-malate + NAD+ | - |
Bos taurus | oxaloacetate + NADH + H+ | - |
r | |
| (S)-malate + NAD+ | - |
Neurospora crassa | oxaloacetate + NADH + H+ | - |
r | |
| (S)-malate + NAD+ | - |
Phycomyces blakesleeanus | oxaloacetate + NADH + H+ | - |
r | |
| (S)-malate + NAD+ | - |
Spinacia oleracea | oxaloacetate + NADH + H+ | - |
r | |
| (S)-malate + NAD+ | - |
Zea mays | oxaloacetate + NADH + H+ | - |
r | |
| (S)-malate + NAD+ | - |
Ascaris suum | oxaloacetate + NADH + H+ | - |
r | |
| (S)-malate + NAD+ | - |
Auxenochlorella pyrenoidosa | oxaloacetate + NADH + H+ | - |
r | |
| (S)-malate + NAD+ | - |
Physarum polycephalum | oxaloacetate + NADH + H+ | - |
r | |
| (S)-malate + NAD+ | - |
Citrus sp. | oxaloacetate + NADH + H+ | - |
r | |
| (S)-malate + NAD+ | - |
Strongylocentrotus purpuratus | oxaloacetate + NADH + H+ | - |
r | |
| (S)-malate + NAD+ | - |
Fundulus heteroclitus | oxaloacetate + NADH + H+ | - |
r | |
| (S)-malate + NAD+ | - |
Gallus sp. | oxaloacetate + NADH + H+ | - |
r | |
| (S)-malate + NAD+ | - |
Drosophila virilis | oxaloacetate + NADH + H+ | - |
r | |
| (S)-malate + NAD+ | - |
Thunnus albacares | oxaloacetate + NADH + H+ | - |
r | |
| (S)-malate + NAD+ | - |
Opuntia ficus-indica | oxaloacetate + NADH + H+ | - |
r | |
| (S)-malate + NAD+ | - |
Salmo sp. | oxaloacetate + NADH + H+ | - |
r | |
| (S)-malate + NAD+ | - |
Tritia obsoleta | oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | - |
Saccharomyces cerevisiae | oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | - |
Bos taurus | oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | - |
Neurospora crassa | oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | - |
Phycomyces blakesleeanus | oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | - |
Spinacia oleracea | oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | - |
Zea mays | oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | - |
Ascaris suum | oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | - |
Auxenochlorella pyrenoidosa | oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | - |
Physarum polycephalum | oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | - |
Citrus sp. | oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | - |
Strongylocentrotus purpuratus | oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | - |
Fundulus heteroclitus | oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | - |
Gallus sp. | oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | - |
Drosophila virilis | oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | - |
Thunnus albacares | oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | - |
Opuntia ficus-indica | oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | - |
Salmo sp. | oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | - |
Tritia obsoleta | oxaloacetate + NADH + H+ | - |
r | |
| oxaloacetate + NADH | - |
Saccharomyces cerevisiae | L-malate + NAD+ | - |
r | |
| oxaloacetate + NADH | - |
Bos taurus | L-malate + NAD+ | - |
r | |
| oxaloacetate + NADH | - |
Neurospora crassa | L-malate + NAD+ | - |
r | |
| oxaloacetate + NADH | - |
Phycomyces blakesleeanus | L-malate + NAD+ | - |
r | |
| oxaloacetate + NADH | - |
Spinacia oleracea | L-malate + NAD+ | - |
r | |
| oxaloacetate + NADH | - |
Zea mays | L-malate + NAD+ | - |
r | |
| oxaloacetate + NADH | - |
Ascaris suum | L-malate + NAD+ | - |
r | |
| oxaloacetate + NADH | - |
Auxenochlorella pyrenoidosa | L-malate + NAD+ | - |
r | |
| oxaloacetate + NADH | - |
Physarum polycephalum | L-malate + NAD+ | - |
r | |
| oxaloacetate + NADH | - |
Citrus sp. | L-malate + NAD+ | - |
r | |
| oxaloacetate + NADH | - |
Strongylocentrotus purpuratus | L-malate + NAD+ | - |
r | |
| oxaloacetate + NADH | - |
Fundulus heteroclitus | L-malate + NAD+ | - |
r | |
| oxaloacetate + NADH | - |
Gallus sp. | L-malate + NAD+ | - |
r | |
| oxaloacetate + NADH | - |
Drosophila virilis | L-malate + NAD+ | - |
r | |
| oxaloacetate + NADH | - |
Thunnus albacares | L-malate + NAD+ | - |
r | |
| oxaloacetate + NADH | - |
Opuntia ficus-indica | L-malate + NAD+ | - |
r | |
| oxaloacetate + NADH | - |
Salmo sp. | L-malate + NAD+ | - |
r | |
| oxaloacetate + NADH | - |
Tritia obsoleta | L-malate + NAD+ | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| s-MDH | - |
Physarum polycephalum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 51 | - |
m-MDH, activity reduced to 50% | Physarum polycephalum |
| 55 | - |
s-MDH, activity reduced to 50% | Physarum polycephalum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
oxaloacetate reduction, s-MDH | Bos taurus |
| 7.6 | - |
oxaloacetate reduction, s-MDH | Gallus sp. |
| 7.6 | - |
oxaloacetate reduction, both isoenzymes | Physarum polycephalum |
| 7.8 | - |
oxaloacetate reduction, m-MDH | Bos taurus |
| 7.8 | - |
oxaloacetate reduction, m-MDH | Gallus sp. |
| 10 | - |
- |
Thunnus albacares |
| 10 | - |
malate oxidation | Physarum polycephalum |
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