Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Moritella sp. |
Protein Variants | Comment | Organism |
---|---|---|
H229Q | mutant enzyme is less resistant to heat denaturation than the wild-type enzyme | Moritella sp. |
Q229H | mutant enzyme is more resistant to heat denaturation than the wild-type enzyme | Moritella sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0135 | - |
NADH | pH 7.0, 30°C, recombinant enzyme | Moritella sp. | |
0.0135 | - |
NADH | enzyme from strain 5710, at pH 7.5 and 30°C | Moritella sp. | |
0.0172 | - |
NADH | pH 7.0, 30°C | Moritella sp. | |
0.0172 | - |
NADH | enzyme from strain 2D2, at pH 7.5 and 30°C | Moritella sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Moritella sp. | - |
- |
- |
Moritella sp. | Q7X3X5 | - |
- |
Moritella sp. 2D2 | Q7X3X5 | - |
- |
Moritella sp. 5710 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Moritella sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | - |
Moritella sp. | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | - |
Moritella sp. 5710 | oxaloacetate + NADH + H+ | - |
r | |
oxaloacetate + NADH + H+ | - |
Moritella sp. | (S)-malate + NAD+ | - |
r | |
oxaloacetate + NADH + H+ | - |
Moritella sp. 2D2 | (S)-malate + NAD+ | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
recombinant enzyme | Moritella sp. |
40 | - |
recombinant enzyme | Moritella sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | - |
midpoint of denaturation for the recombinant enzyme | Moritella sp. |
45 | - |
midpoint of denaturation for the recombinant enzyme | Moritella sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00386 | - |
oxaloacetate | pH 7.0, 30°C, recombinant enzyme | Moritella sp. | |
0.00483 | - |
oxaloacetate | pH 7.0, 30°C | Moritella sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | reduction of oxaloacetate | Moritella sp. |
7.5 | 8 | reduction of oxaloacetate, recombinant enzyme | Moritella sp. |
9.5 | 10 | dehydrogenation of malate | Moritella sp. |
9.5 | 10 | dehydrogenation of malate, recombinant enzyme | Moritella sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Moritella sp. | |
NADH | - |
Moritella sp. |