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Literature summary for 1.1.1.37 extracted from

  • Saito, R.; Nakayama, A.
    Differences in malate dehydrogenases from the obligately piezophilic deep-sea bacterium Moritella sp. strain 2D2 and the psychrophilic bacterium Moritella sp. strain 5710 (2004), FEMS Microbiol. Lett., 233, 165-172.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Moritella sp.

Protein Variants

Protein Variants Comment Organism
H229Q mutant enzyme is less resistant to heat denaturation than the wild-type enzyme Moritella sp.
Q229H mutant enzyme is more resistant to heat denaturation than the wild-type enzyme Moritella sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0135
-
NADH pH 7.0, 30°C, recombinant enzyme Moritella sp.
0.0135
-
NADH enzyme from strain 5710, at pH 7.5 and 30°C Moritella sp.
0.0172
-
NADH pH 7.0, 30°C Moritella sp.
0.0172
-
NADH enzyme from strain 2D2, at pH 7.5 and 30°C Moritella sp.

Organism

Organism UniProt Comment Textmining
Moritella sp.
-
-
-
Moritella sp. Q7X3X5
-
-
Moritella sp. 2D2 Q7X3X5
-
-
Moritella sp. 5710
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Moritella sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NAD+
-
Moritella sp. oxaloacetate + NADH + H+
-
r
(S)-malate + NAD+
-
Moritella sp. 5710 oxaloacetate + NADH + H+
-
r
oxaloacetate + NADH + H+
-
Moritella sp. (S)-malate + NAD+
-
r
oxaloacetate + NADH + H+
-
Moritella sp. 2D2 (S)-malate + NAD+
-
r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
recombinant enzyme Moritella sp.
40
-
recombinant enzyme Moritella sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
42
-
midpoint of denaturation for the recombinant enzyme Moritella sp.
45
-
midpoint of denaturation for the recombinant enzyme Moritella sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00386
-
oxaloacetate pH 7.0, 30°C, recombinant enzyme Moritella sp.
0.00483
-
oxaloacetate pH 7.0, 30°C Moritella sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8 reduction of oxaloacetate Moritella sp.
7.5 8 reduction of oxaloacetate, recombinant enzyme Moritella sp.
9.5 10 dehydrogenation of malate Moritella sp.
9.5 10 dehydrogenation of malate, recombinant enzyme Moritella sp.

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Moritella sp.
NADH
-
Moritella sp.