Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.37 extracted from

  • Lopez-Calcagno, P.E.; Moreno, J.; Cedeno, L.; Labrador, L.; Concepcion, J.L.; Avilan, L.
    Cloning, expression and biochemical characterization of mitochondrial and cytosolic malate dehydrogenase from Phytophthora infestans (2009), Mycol. Res., 113, 771-781.
    View publication on PubMed
Search for more literature for 1.1.1.37

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21pLys cells Phytophthora infestans

Inhibitors

Inhibitors Comment Organism Structure
oxaloacetate
-
 Phytophthora infestans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.022
-
 oxaloacetate recombinant wild type mitochondrial MDH, at 25°C Phytophthora infestans
0.026
-
 NADH recombinant wild type cytosolic MDH, at 25°C Phytophthora infestans
0.035
-
 oxaloacetate native wild type mitochondrial MDH, at 25°C Phytophthora infestans
0.086
-
 NADH recombinant wild type mitochondrial MDH, at 25°C Phytophthora infestans
0.176
-
 oxaloacetate recombinant wild type cytosolic MDH, at 25°C Phytophthora infestans
0.21
-
 NAD+ native wild type mitochondrial MDH, at 25°C Phytophthora infestans
0.21
-
 NADH native wild type mitochondrial MDH, at 25°C Phytophthora infestans
0.21
-
 NAD+ recombinant wild type cytosolic MDH, at 25°C Phytophthora infestans
0.37
-
 NAD+ recombinant wild type mitochondrial MDH, at 25°C Phytophthora infestans
0.64
-
 (S)-malate recombinant wild type mitochondrial MDH, at 25°C Phytophthora infestans
0.94
-
 (S)-malate native wild type mitochondrial MDH, at 25°C Phytophthora infestans
1.72
-
 (S)-malate recombinant wild type cytosolic MDH, at 25°C Phytophthora infestans

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Phytophthora infestans 5829
-
mitochondrion
-
 Phytophthora infestans 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35400
-
 subunit of mitochondrial MDH, calculated from amino acid sequence Phytophthora infestans
36400
-
 subunit of cytosolic MDH, calculated from amino acid sequence Phytophthora infestans
41000
-
 subunit of mitochondrial MDH, SDS-PAGE Phytophthora infestans
42000
-
 subunit of cytosolic MDH, SDS-PAGE Phytophthora infestans
45000
-
 2 * 45000, gel filtration Phytophthora infestans
90000
-
 cytosolic MDH and mitochondrial MDH, gel filtration Phytophthora infestans

Organism

Organism UniProt Comment Textmining
Phytophthora infestans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate fractionation and Cibacron Blue column chromatography Phytophthora infestans

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Phytophthora infestans
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NAD+
-
 Phytophthora infestans oxaloacetate + NADH + H+
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 45000, gel filtration Phytophthora infestans

Synonyms

Synonyms Comment Organism
MDH
-
 Phytophthora infestans

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Phytophthora infestans

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.25
-
 oxaloacetate recombinant wild type mitochondrial MDH, at 25°C Phytophthora infestans
2.8
-
 oxaloacetate native wild type mitochondrial MDH, at 25°C Phytophthora infestans
12.3
-
 oxaloacetate recombinant wild type cytosolic MDH, at 25°C Phytophthora infestans

pI Value

Organism Comment pI Value Maximum pI Value
Phytophthora infestans mitochondrial MDH, calculated from amino acid sequence
-
 5.9
Phytophthora infestans cytosolic MDH, calculated from amino acid sequence
-
 6.2