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Literature summary for 1.1.1.37 extracted from

  • Langelandsvik, A.S.; Steen, I.H.; Birkeland, N.K.; Lien, T.
    Properties and primary structure of a thermostable L-malate dehydrogenase from Archaeoglobus fulgidus (1997), Arch. Microbiol., 168, 59-67.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Archaeoglobus fulgidus

Inhibitors

Inhibitors Comment Organism Structure
(S)-malate product inhibitor Archaeoglobus fulgidus
NADH substrate inhibition Archaeoglobus fulgidus
oxaloacetate substrate inhibition Archaeoglobus fulgidus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.024
-
NADH pH 8.0, 65°C, at 0.4 mM oxaloacetate Archaeoglobus fulgidus
0.043
-
oxaloacetate pH 8.0, 65°C, at 0.4 mM NADH Archaeoglobus fulgidus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
2 * 35000, SDS-PAGE Archaeoglobus fulgidus
70000
-
gel filtration Archaeoglobus fulgidus

Organism

Organism UniProt Comment Textmining
Archaeoglobus fulgidus O08349
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Archaeoglobus fulgidus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.64
-
pH 8.0, 65°C, cell extract Archaeoglobus fulgidus
4799
-
pH 8.0, 65°C, purified enzyme Archaeoglobus fulgidus

Storage Stability

Storage Stability Organism
4°C, stable for several months under oxic conditions Archaeoglobus fulgidus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NAD+ the reduction of oxaloacetate with NADH is preferred. Malate is oxidized by NAD+ at 10% of the maximal velocity for the reduction of oxaloacetate with NADH. No oxidation of malate with NADP+ as coenzyme Archaeoglobus fulgidus oxaloacetate + NADH + H+
-
r
oxaloacetate + NADH + H+ the reduction of oxaloacetate with NADH is preferred. Malate is oxidized by NAD+ at 10% of the maximal velocity for the reduction of oxaloacetate with NADH. Maximal activity with NADPH is 10% compared to the activity with NADH Archaeoglobus fulgidus (S)-malate + NAD+
-
r

Subunits

Subunits Comment Organism
homodimer 2 * 35000, SDS-PAGE Archaeoglobus fulgidus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
assay at Archaeoglobus fulgidus
90
-
-
Archaeoglobus fulgidus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 85 between 50°C and 85°C the activity increases following the Arrhenius equation with a calculated activation energy of 61.6 kJ/mol Archaeoglobus fulgidus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
5 h, stable Archaeoglobus fulgidus
101
-
half-life: more than 80 min. Marked stabilizing effect of 1 M K2HPO4 with an increase in thermostability of approximately 40% at 101°C Archaeoglobus fulgidus
102
-
half-life: 30 min Archaeoglobus fulgidus
103
-
half-life: 18 min Archaeoglobus fulgidus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6 8.2 in both 50 mM Tricine-KOH and 50 mM potassium phosphate buffer Archaeoglobus fulgidus
8
-
assay at Archaeoglobus fulgidus

Cofactor

Cofactor Comment Organism Structure
NAD+ no oxidation of malate with NADP+ as coenzyme Archaeoglobus fulgidus
NADH maximal activity with NADPH is 10% compared to the activity with NADH Archaeoglobus fulgidus
NADPH maximal activity with NADPH is 10% compared to the activity with NADH Archaeoglobus fulgidus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2
-
(S)-malate pH 8.0, 65°C Archaeoglobus fulgidus

pI Value

Organism Comment pI Value Maximum pI Value
Archaeoglobus fulgidus isoelectric focusing, pH 3–10
-
4.5