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Literature summary for 1.1.1.376 extracted from

  • Aro-Kaerkkaeinen, N.; Toivari, M.; Maaheimo, H.; Ylilauri, M.; Pentikaeinen, O.T.; Andberg, M.; Oja, M.; Penttilae, M.; Wiebe, M.G.; Ruohonen, L.; Koivula, A.
    L-arabinose/D-galactose 1-dehydrogenase of Rhizobium leguminosarum bv. trifolii characterised and applied for bioconversion of L-arabinose to L-arabonate with Saccharomyces cerevisiae (2014), Appl. Microbiol. Biotechnol., 98, 9653-9665.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae Rhizobium leguminosarum bv. trifolii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
NADP+ L-arabinose as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C Rhizobium leguminosarum bv. trifolii
0.33
-
L-arabinose with NADP+ as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C Rhizobium leguminosarum bv. trifolii
0.48
-
D-galactose with NAD+ as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C Rhizobium leguminosarum bv. trifolii
0.5
-
D-galactose with NADP+ as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C Rhizobium leguminosarum bv. trifolii
0.77
-
NAD+ L-arabinose as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C Rhizobium leguminosarum bv. trifolii
0.93
-
L-arabinose with NAD+ as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C Rhizobium leguminosarum bv. trifolii
1.3
-
D-fucose with NADP+ as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C Rhizobium leguminosarum bv. trifolii
2.72
-
D-fucose with NAD+ as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C Rhizobium leguminosarum bv. trifolii
23.39
-
D-talose with NAD+ as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C Rhizobium leguminosarum bv. trifolii
57.3
-
D-talose with NADP+ as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C Rhizobium leguminosarum bv. trifolii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34800
-
-
Rhizobium leguminosarum bv. trifolii
67600
-
gel filtration Rhizobium leguminosarum bv. trifolii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-arabinose + NAD+ Rhizobium leguminosarum bv. trifolii
-
L-arabinono-1,4-lactone + NADH + H+
-
?
L-arabinose + NADP+ Rhizobium leguminosarum bv. trifolii
-
L-arabinono-1,4-lactone + NADPH + H+
-
?

Organism

Organism UniProt Comment Textmining
Rhizobium leguminosarum bv. trifolii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
StrepTactin Sepharose column chromatography Rhizobium leguminosarum bv. trifolii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fucose + NAD+
-
Rhizobium leguminosarum bv. trifolii D-fucono-1,4-lactone + NADH + H+
-
r
D-fucose + NADP+ 100% activity Rhizobium leguminosarum bv. trifolii ?
-
?
D-fucose + NADP+
-
Rhizobium leguminosarum bv. trifolii D-fucono-1,4-lactone + NADPH + H+
-
r
D-galactose + NAD+
-
Rhizobium leguminosarum bv. trifolii D-galactono-1,4-lactone + NADH + H+
-
r
D-galactose + NADP+ 69% activity compared to D-fucose Rhizobium leguminosarum bv. trifolii ?
-
?
D-galactose + NADP+
-
Rhizobium leguminosarum bv. trifolii D-galactono-1,4-lactone + NADPH + H+
-
r
D-talose + NAD+
-
Rhizobium leguminosarum bv. trifolii D-talono-1,4-lactone + NADH + H+
-
r
D-talose + NADP+ 12% activity compared to D-fucose Rhizobium leguminosarum bv. trifolii ?
-
?
D-talose + NADP+
-
Rhizobium leguminosarum bv. trifolii D-talono-1,4-lactone + NADPH + H+
-
r
L-arabinose + NAD+
-
Rhizobium leguminosarum bv. trifolii L-arabinono-1,4-lactone + NADH + H+
-
?
L-arabinose + NADP+
-
Rhizobium leguminosarum bv. trifolii L-arabinono-1,4-lactone + NADPH + H+
-
?
L-arabinose + NADP+ 21% activity compared to D-fucose Rhizobium leguminosarum bv. trifolii L-arabinono-1,4-lactone + NADPH + H+
-
?
additional information some minor activity (about 1%) is measured with D-xylose and L-lyxose, while no activity is detected with D-glucose or L-rhamnose Rhizobium leguminosarum bv. trifolii ?
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 33526, calculated from amino acid sequence Rhizobium leguminosarum bv. trifolii
homodimer 2 * 34800, SDS-PAGE Rhizobium leguminosarum bv. trifolii

Synonyms

Synonyms Comment Organism
AraDH
-
Rhizobium leguminosarum bv. trifolii
L-arabinose/D-galactose 1-dehydrogenase
-
Rhizobium leguminosarum bv. trifolii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
20.85
-
L-arabinose with NADP+ as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C Rhizobium leguminosarum bv. trifolii
35.45
-
NADP+ L-arabinose as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C Rhizobium leguminosarum bv. trifolii
38.12
-
D-galactose with NAD+ as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C Rhizobium leguminosarum bv. trifolii
65.55
-
L-arabinose with NAD+ as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C Rhizobium leguminosarum bv. trifolii
69.9
-
D-galactose with NADP+ as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C Rhizobium leguminosarum bv. trifolii
74.58
-
D-talose with NAD+ as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C Rhizobium leguminosarum bv. trifolii
85.73
-
NAD+ L-arabinose as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C Rhizobium leguminosarum bv. trifolii
99.52
-
D-talose with NADP+ as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C Rhizobium leguminosarum bv. trifolii
110.1
-
D-fucose with NADP+ as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C Rhizobium leguminosarum bv. trifolii
120.4
-
D-fucose with NAD+ as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C Rhizobium leguminosarum bv. trifolii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Rhizobium leguminosarum bv. trifolii

Cofactor

Cofactor Comment Organism Structure
NAD+ the enzyme prefers NADP+ but uses also NAD+ as a cofactor Rhizobium leguminosarum bv. trifolii
NADP+ the enzyme prefers NADP+ but uses also NAD+ as a cofactor Rhizobium leguminosarum bv. trifolii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.8
-
D-talose with NADP+ as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C Rhizobium leguminosarum bv. trifolii
3.3
-
D-talose with NAD+ as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C Rhizobium leguminosarum bv. trifolii
44.3
-
D-fucose with NAD+ as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C Rhizobium leguminosarum bv. trifolii
63.5
-
L-arabinose with NADP+ as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C Rhizobium leguminosarum bv. trifolii
70.7
-
L-arabinose with NAD+ as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C Rhizobium leguminosarum bv. trifolii
80.2
-
D-galactose with NAD+ as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C Rhizobium leguminosarum bv. trifolii
85.3
-
D-fucose with NADP+ as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C Rhizobium leguminosarum bv. trifolii
111.8
-
NAD+ L-arabinose as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C Rhizobium leguminosarum bv. trifolii
140
-
D-galactose with NADP+ as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C Rhizobium leguminosarum bv. trifolii
741.7
-
NADP+ L-arabinose as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C Rhizobium leguminosarum bv. trifolii